O presente estudo teve por objetivo investigar a influência da aplicação de lactato de sódio, ácido lático e ácido cítrico nas propriedades físico-químicas e microbiológicas do camarão (Litopenaeusvannamei). As amostras foram submetidas, logo após a coleta, ao processamento mínimo e dois tratamentos distintos: ausência de imersão em solução de ácidos orgânicos e lactato de sódio (controle) e imersão na solução de bioconservantes (tratado). As porções foram embaladas a vácuo e estocadas sob refrigeração. Durante o armazenamento, foram realizadas análises físico-químicas tais como o potencial hidrogeniônico, a capacidade de retenção de água, a perda de peso por cocção e cor. Foram também realizadas análises microbiológicas para contagem de bactérias aeróbicas mesófilas e psicrotróficas nos dias zero, dois e quatro, em três repetições por tratamento. Os dados obtidos foram avaliados pelo teste de t Student ao nível de 5% de significância. Os resultados obtidos comprovaram a eficácia do tratamento na preservação da qualidade do camarão, onde os resultados obtidos nas análises físico-químicas apresentaram melhores valores no grupo tratado quando comparado ao grupo controle, assim como para as análises bacteriológicas onde os resultados mostraram uma redução significativa no crescimento de microrganismos no grupo tratado quando comparado com o grupo controle.

This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199+) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP‐15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p < .05); however, this result is similar to 1 ng/ml BMP‐15 (74.00% ± 1.90, p > .05). Moreover, 25 and 50 ng/ml of BMP‐15 promoted follicular activation. BMP‐15 supplements did not affect oocyte and follicular growth. All concentrations of BMP‐15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p < .05). However, at the end of the experiment, the number of NORs in follicles cultured in all treatments was higher than that observed in the fresh control (sample taken prior to culturing) (p > .05). In summary, the addition of 25 ng/ml BMP‐15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture.

This study evaluated the effect of different concentrations of Brazilian green propolis on the cryopreservation of epididymal sperm from domestic cats. Spermatozoa were collected from cauda epididymis by slicing technique in a TRIS extender, preheated to 37ºC. Then, the sperm were evaluated and divided randomly between the control group (without adding propolis in the extender) and the treatment groups (adding different concentrations of propolis extract in the extender): P1 (0.1 mg/mL), P2 (0.3 mg/mL) e P3 (0.6 mg/mL). Subsequently, sperm were cryopreserved. The evaluated parameters were sperm kinetics by the Computer Assisted Semen Analysis, vigor, viability, membrane functionality, chromatin condensation and morphology. The parameters were measured before and after cryopreservation. Propolis didn’t show toxicity to the sperm in any concentrations, with no changes observed in the motility pattern. There was no significant influence of propolis on motility, vigor, percentage of viable spermatozoa, chromatin quality and other kinetic parameters in the cryopreserved samples. In sperm morphology, it was demonstrated a reduction in the percentage of normal cells in P1 and P2 groups in relation to the fresh sample, control and P3 group. In addition, an interesting effect from the propolis groups was observed on possible growth inhibition of microorganisms in the contaminated samples. Propolis didn’t provide superiority to the sperm parameters evaluated after thawing, except for the values of plasma membrane functionality, which were better. The propolis is an interesting component to be incorporated in the cryoprotectant medium due to its non-toxicity and the potential inhibition of microbial growth.

The objective of the research was to diagnose the presence of parasites in coriander (Coriandrum sativum L.) sold in supermarkets and open markets in the city of Mossoró, State of Rio Grande do Norte. In total, 40 samples of coriander were analyzed, 20 of which were sold at open markets and 20 from supermarkets, in search of parasitic structures using the spontaneous sedimentation technique, followed by microscopic analysis. Four supermarkets and four stalls at an open market were randomly analyzed. Among the 40 samples analyzed, 27.5% (11/40) were positive for parasitological contamination, of which 81.8% (9/11) came from open markets and 18.2% (2/11) supermarkets. Among the positive samples, parasitic structures of the genera Ancylostoma sp., Toxocara sp., and Strongyloides sp. Thus, it is concluded that the results demonstrate that a considerable index of the samples is unsuitable for human consumption, and may cause harm to the health of the consumer.