Spermatogenesis and Testicular Cycle in Rough Greensnakes,<i>Opheodrys aestivus</i>, from Arkansas

Konvalina, John D.; Trauth, Stanley E.; Plummer, Michael V.

doi:10.1670/16-163

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…Moreover, a Each value represents mean ± SE * Significant differences versus control at P ≤ 0.05 ** Significant differences versus control at P ≤ 0.01 significant reduction in the testes' weight after exposure to atrazine [8]. sperm count is considered a crucial sensitive test used for the evaluation of spermatogenesis because it is highly correlated with fertility [34]. Our study revealed that rats treated with CPF had markedly impaired sperm quality.…”

Section: Discussionmentioning

confidence: 92%

The Reproductive Injury and Oxidative Testicular Toxicity Induced by Chlorpyrifos Can Be Restored by Zinc in Male Rats

Khalaf

Ogaly

Ibrahim

et al. 2021

Biol Trace Elem Res

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The current study aimed to evaluate the harmful effect of chlorpyrifos (CPF) on the reproductive functions and fertility in male rats and to assess the protective role of zinc (Zn) in improving the adverse effects of CPF on male fertility. Sixty mature male rats were divided into four groups: Group 1: The control group was orally administered with the corresponding dose of corn oil. Group 2 animals received chlorpyrifos (1 mg/kg, oral). Group 3 rats received oral zinc (25 mg/kg) daily. Group 4 animals received oral zinc treatment (25 mg/kg). CPF caused a significant decrease in the body and reproductive organs' weights, sperm count, sperm motility percent, serum testosterone, FSH, and LH. The CPF-treated group showed a significant increase in dead sperm percent and sperm abnormalities. CPF induced a significant internucleosomal DNA fragmentation and marked histological alterations in the testes of treated male rats. Conversely, co-treatment with Zn improved the reproductive organs weights, sperm characteristics, internucleosomal DNA fragmentation, and histological alterations of the testes. In conclusion, CPF triggered significant detrimental effects on male reproductive organs and functions and the co-treatment with zinc partly alleviate the injurious effects of CPF on male fertility.

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Section: Discussionmentioning

confidence: 92%

The Reproductive Injury and Oxidative Testicular Toxicity Induced by Chlorpyrifos Can Be Restored by Zinc in Male Rats

Khalaf

Ogaly

Ibrahim

et al. 2021

Biol Trace Elem Res

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“…Spermatogenesis of L. ocellata is triphasic, based on functional and morphological criteria, similar to other vertebrates (Gribbins, ; Gribbins et al, ; Gribbins, Elsey, & Gist, ; Gribbins, Gist, & Congdon, ; Gribbins, Rheubert, Collier, Siegel, & Sever, ; Konvalina, Trauth, & Plummer, ; Lancaster, Trauth, & Gribbins, ; Villagrán‐SantaCruz et al, ). Stages of spermatogenesis, which represent specific cell association from different types of developing germ cells that undergo successive progression in a repetitive pattern to form a cycle of the seminiferous epithelium, are well described in mammals and birds, but are poorly understood in the reptilian testes (Gribbins & Rheubert, ).…”

Section: Discussionmentioning

confidence: 75%

“…Unlike mammalian acrosomal vesicles that are detectable using PAS histochemistry, this technique could not be applied to stain the acrosomal vesicles of L. ocellata for the identification of the spermatid steps due to poor staining properties of these subcellular structures, as also described previously (Lancaster et al, ). Gribbins () has suggested that the number of reptilian spermatid steps is relatively consistent, with seven steps (Gribbins & Gist, ; Gribbins et al, , , ; Gribbins, Happ, & Sever, ; Hernández‐Franyutti & Uribe, ; Konvalina et al, ; Lancaster et al, ; Rheubert, Mchugh, Collier, Sever, & Gribbins, ; Rheubert, Poldemann, et al, ; Siegel et al, ; Villagrán‐SantaCruz et al, ), eight steps (Gribbins et al, , ; Wang, Wu, Xu, Wang, & Wang, ) or uniquely 10 steps (Sousa et al, ). Likewise, spermatids of L. ocellata are classified into eight ontogenetic steps.…”

Section: Discussionmentioning

confidence: 99%

Microanatomy of the testes and testicular ducts of the butterfly lizard, Leiolepis ocellata Peters, 1971 (Reptilia: Squamata: Agamidae) during the active reproductive period

Pewhom

Srakaew

2018

Acta Zoologica

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The microanatomy of the testes and testicular ducts (rete testis, ductuli efferentes, ductus epididymis and ductus deferens) of Leiolepis ocellata (Agamidae) was investigated using light microscopy including histochemistry. Each testis contains seminiferous tubules and interstitial tissues. The former house spermatogenic cells (spermatogonia A & B, preleptotene, primary and secondary spermatocytes, spermatids (steps 1–8) and spermatozoa) and Sertoli cells, while the latter comprise peritubular and intersitial tissues. The rete testis is an anastomosing duct, having intratesticular and extratesticular portions. The proximal region of ductuli efferentes has wider outer ductal and luminal diameters than those of the distal region. The convoluted ductus epididymis is subdivided into four regions (initial segment, caput, corpus and cauda), based on the ductal diameter, epithelium characteristics and cell components. The ductus deferens has the greatest diameter and is divided into the ductal and ampulla ductus deferens. The ductal portion is subdivided into the proximal and distal regions, based on the epithelium types and ductal diameters. The ampulla ductus deferens is a fibromuscular tube, having numerous mucosal folds projecting into the lumen. Spermiophagy is detectable in the ductus epididymis and ductus deferens. The present results contribute to improved fundamental knowledge on the microanatomy of the reptilian reproductive system.

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“…The annual reproductive cycle of male Leiolepis ocellata was divided into eight periods, based on changes of plasma sex steroid levels, and histomorphological and histochemical characteristics of the reproductive structures, unlike other reptiles including Hemidactylus flaviviridis [ 17 ] and Ophisops elegans [ 38 ] with three periods; Opheodrys aestivus [ 39 ], Sceloporus aeneus [ 27 ], and S. mucronatus [ 40 ] with four periods; Microgecko helenae (formerly Tropiocolotes helenae ) with five periods [ 38 ]; and Dipsas mikanii (formerly Sibynomorphus mikanii ) with six periods [ 31 ]. In addition, the reproductive cycle of L. ocellata exhibited a biannual/bimodal pattern of the reproductive cycle, having two peaks of the active periods, as in other reptiles, including Agkistrodon contortrix [ 41 ], A. piscivorus [ 32 ], A. piscivorus leucostoma [ 42 ], Masticophis bilineatus [ 43 ], Sceloporus spinosus [ 44 ], and Vipera aspis [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Male reproductive cycle in a population of the endemic butterfly lizard, Leiolepis ocellata Peters, 1971 (Squamata: Agamidae) from northern Thailand

Pewhom¹,

Supapakorn²,

Srakaew³

2022

BMC Zool

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Background Fundamental knowledge on the seasonal reproductive microanatomy and endocrinology of reptiles has been collected from several studies of various species. The present study was to determine annual changes in hormonal profiles, and detailed histomorphometric and histochemical characteristics of the entire male reproductive system of the tropical agamid lizard, Leiolepis ocellata. Results Male L. ocellata individuals (n = 75) collected from the territory of two provinces (Lampang and Tak) in northern Thailand exhibited annual variation in sex hormonal, histomorphometric, and histochemical characteristics of the male reproductive system. The reproductive cycle was subdivided into eight reproductive periods (early first active, first active, resting, second recrudescent, second active, regressive, quiescent, and first recrudescent), thus displaying a bimodal pattern with two actively reproductive periods. Circulating sex hormones (testosterone, estradiol, and progesterone) peaked in the first active (February) and the second active (June–July) periods. Likewise, gonadosomatic index (GSI) and histomorphometric variables of the testes and of the genital ducts (rete testis, ductuli efferentes, ductus epididymis, and ductus deferens) revealed their highest values in the first active period. Marked increase in protein and carbohydrate production was detectable in the ductuli efferentes during the active periods. Conclusions The male reproductive cycle of L. ocellata showed a biannual pattern of the hormonal profile, and detailed histomorphometric and histochemical characteristics of the entire reproductive system. Hence, the present study provides improved basic knowledge on the reptilian reproductive biology with comparative viewpoints to other reptiles.

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Spermatogenesis and Testicular Cycle in Rough Greensnakes,Opheodrys aestivus, from Arkansas

Cited by 5 publications

References 10 publications

The Reproductive Injury and Oxidative Testicular Toxicity Induced by Chlorpyrifos Can Be Restored by Zinc in Male Rats

The Reproductive Injury and Oxidative Testicular Toxicity Induced by Chlorpyrifos Can Be Restored by Zinc in Male Rats

Microanatomy of the testes and testicular ducts of the butterfly lizard, Leiolepis ocellata Peters, 1971 (Reptilia: Squamata: Agamidae) during the active reproductive period

Male reproductive cycle in a population of the endemic butterfly lizard, Leiolepis ocellata Peters, 1971 (Squamata: Agamidae) from northern Thailand

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