Spermatogenesis in the Microminipig

Kangawa, Akihisa; Otake, Masayoshi; Enya, Satoko; Yoshida, Toshinori; Kangawa, Yumi; Shibata, Masatoshi

doi:10.1177/0192623316654586

Cited by 14 publications

(14 citation statements)

References 47 publications

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“…The mean diameter of seminiferous tubules was estimated from 30 randomly chosen round tubules in each testis, independent of their cycle stage. In animals that were developed enough for the tubular lumen to be recognizable, the height of the seminiferous epithelium was determined from 10 round tubules at stages VII and VIII, which were readily identified with H&E. Stage classification was based on cellular associations (Kangawa et al 2016). For example, germ cells from stage VII tubules were composed of type A spermatogonia, preleptotene and pachytene spermatocytes, and round and elongated spermatids, whereas those from stage VIII tubules were composed of type A spermatogonia, leptotene and pachytene spermatocytes, and round spermatids.…”

Section: Morphometry In Seminiferous Tubules and Ductus Epididymismentioning

confidence: 99%

Histological Development of Male Reproductive Organs in Microminipigs

et al. 2016

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Microminipigs are becoming increasingly attractive alternatives for various experimental applications, such as general toxicology studies, owing to their manageable size. However, there are limited studies on the male reproductive organs of microminipigs, particularly on the histological aspects of sexual maturity. To clarify the development of male reproductive organs, 35 male microminipigs, aged 0 to 12 months, were used in this study. Histological and histomorphological evaluation was performed based on spermatogenic development, measurement of tubular structure in testes and epididymides, and histological progress of accessory glands. In addition, spontaneous testicular changes were quantitatively assessed. Histologically, male microminipigs sexually matured around 4.5 months of age, when spermatogenesis in testes and structural development in genital organs were completed. Spontaneous testicular changes occurred in all the animals investigated. Multinucleated giant cell was most commonly observed, followed by hypospermatogenesis and tubular atrophy/hypoplasia. However, the number of affected tubules was less than 1% in testes after 4.5 months of age, suggesting that the influence of these changes on evaluation of toxicity studies may be minimal. It is preferable to use sexually mature animals in toxicology studies; therefore, the information obtained by the present study will be helpful for future toxicity evaluations in microminipigs.

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Section: Morphometry In Seminiferous Tubules and Ductus Epididymismentioning

confidence: 99%

Histological Development of Male Reproductive Organs in Microminipigs

et al. 2016

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“…The testicles of rat 6 , mouse 7 , and dog 8,9 are commonly investigated based on the latter system, as these are the most frequently used species in research as well as in toxicology testing 8 . Nevertheless, seminiferous staging on the basis of acrosome development during spermiogenesis has been described in many other species, including monkeys [10][11][12][13][14] , guinea pigs 15 , hamsters 16,17 , gerbils 18 , minks 19 , pigs 20,21 , opossums 22 , armadillos 23,24 , quails 25 , turtles 26 , and bulls 3 .…”

Section: Introductionmentioning

confidence: 99%

Seminiferous epithelium cycle staging based on the development of the acrosome in ram testis

et al. 2021

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Testicular histopathology is considered the most sensitive and reliable method to detect the effects of chemicals on sperm production. To carry out a sensitive examination of testicular histopathology and interpret the changes require knowledge of spermatogenic stages. Spermatogenic staging based on acrosome development during spermiogenesis is conventionally performed in animal species routinely used for research and toxicity testing. In contrast, small ruminants, such as sheep and goats, are rarely used as animal models to evaluate toxicity in male reproductive organs. To the best of our knowledge, a comparable spermatogenic staging system in rams has not yet been fully characterised. Hence, this study aimed to adapt the existing spermatogenic staging based on acrosome development in bull testes to fit the seminiferous epithelium cycle of ram testes. The results show that spermatogenic staging based on acrosome development in bull testes can, with slight modifications, be efficiently used for the staging of ram testes.

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“…An 8-stage method (PAS and H&E) to assess spermatogenesis in the Göttingen minipig has been proposed, 141,142 and Kangawa et al have published a staging method in the microminipig using an 11-stage method (PAS). 143 Some further guidelines may be inferred from literature detailing reproductive maturation of the testes of various breeds of domestic swine, but at this time, stage-aware evaluation is not routinely performed for this species and there are no clear, comprehensive published guidelines for performance of this evaluation in the Göttingen minipig.…”

Section: Male Minipig Reproductive Anatomy Histology and Examinatiomentioning

confidence: 99%

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Minipig

et al. 2021

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The INHAND (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions) Project ( www.toxpath.org/inhand.asp ) is a joint initiative of the Societies of Toxicologic Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP), and North America (STP) to develop an internationally accepted nomenclature for proliferative and nonproliferative lesions in laboratory animals. The purpose of this publication is to provide a standardized nomenclature for classifying microscopic lesions observed in most tissues and organs from the minipig used in nonclinical safety studies. Some of the lesions are illustrated by color photomicrographs. The standardized nomenclature presented in this document is also available electronically on the internet ( http://www.goreni.org/ ). Sources of material included histopathology databases from government, academia, and industrial laboratories throughout the world. Content includes spontaneous lesions as well as lesions induced by exposure to test materials. Relevant infectious and parasitic lesions are included as well. A widely accepted and utilized international harmonization of nomenclature for lesions in laboratory animals will provide a common language among regulatory and scientific research organizations in different countries and increase and enrich international exchanges of information among toxicologists and pathologists.

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Spermatogenesis in the Microminipig

Cited by 14 publications

References 47 publications

Histological Development of Male Reproductive Organs in Microminipigs

Histological Development of Male Reproductive Organs in Microminipigs

Seminiferous epithelium cycle staging based on the development of the acrosome in ram testis

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Minipig

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