Spermatogonial Stem Cells and Animal Transgenesis

Barros, Flávia Regina Oliveira de; Giassetti, Mariana Ianello; Visintin, José Antônio

doi:10.5772/28960

Cited by 4 publications

(3 citation statements)

References 99 publications

(51 reference statements)

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“…SSCs are male germline stem cells with indefinite self-renewal ability ( Hofmann, 2008 ; Luo et al, 2009 ; Kanatsu-Shinohara and Shinohara, 2013 ) and are unipotent with respect to their differentiating into sperm through spermatogenesis in seminiferous tubule in the testis ( Aponte et al, 2005 ; Luo et al, 2009 ; Lee et al, 2013 ). Simultaneously, SSCs can acquire pluripotency differentiating into three germ-layer cells by external specific stimulation ( Guan et al, 2006 ; Simon et al, 2010 ; Lim et al, 2013 ) and produce transgenic sperms by introducing target genes into cells ( Nagano et al, 2001 ; Lavitrano et al, 2006; de Barros et al, 2012 ). Accordingly, they serve as a useful tool for understanding spermatogenesis ( Oatley and Brinster, 2008 ; Phillips et al, 2010 ; Kanatsu-Shinohara and Shinohara, 2013 ), preserving male reproduction ( Phillips et al, 2010 ), overcoming male infertility ( Kubota and Brinster, 2006 ; Vlajković et al, 2012 ), conducting patient-specific cell therapy ( Daley and Scadden, 2008 ; Vlajković et al, 2012 ), and producing transgenic animals ( de Barros et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis

Park

Kim

et al. 2016

Asian Australas. J. Anim. Sci

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In general, the seminiferous tubule basement membrane (STBM), comprising laminin, collagen IV, perlecan, and entactin, plays an important role in self-renewal and spermatogenesis of spermatogonial stem cells (SSCs) in the testis. However, among the diverse extracellular matrix (ECM) proteins constituting the STBM, the mechanism by which each regulates SSC fate has yet to be revealed. Accordingly, we investigated the effects of various ECM proteins on the maintenance of the undifferentiated state of SSCs in pigs. First, an extracellular signaling-free culture system was optimized, and alkaline phosphatase (AP) activity and transcriptional regulation of SSC-specific genes were analyzed in porcine SSCs (pSSCs) cultured for 1, 3, and 5 days on non-, laminin- and collagen IV-coated Petri dishes in the optimized culture system. The microenvironment consisting of glial cell-derived neurotrophic factor (GDNF)-supplemented mouse embryonic stem cell culture medium (mESCCM) (GDNF-mESCCM) demonstrated the highest efficiency in the maintenance of AP activity. Moreover, under the established extracellular signaling-free microenvironment, effective maintenance of AP activity and SSC-specific gene expression was detected in pSSCs experiencing laminin-derived signaling. From these results, we believe that laminin can serve as an extracellular niche factor required for the in vitro maintenance of undifferentiated pSSCs in the establishment of the pSSC culture system.

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Section: Introductionmentioning

confidence: 99%

Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis

Park

Kim

et al. 2016

Asian Australas. J. Anim. Sci

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“…Studying SSCs population and associated niche in vitro and also genetically manipulation and transplantation of these SSC further provide a model to better understand adult stem cell biology, decipher the mechanisms that control SSC functions and modify the germ line for transgenesis [8][9][10][11][12][13]. Since, SSCs are present in extremely low numbers, the ability to study their self-renewal and biological characteristics requires large enough populations of pure SSCs and an in vitro system that supports this process in enriched biologically active populations of cells [2,14].…”

Section: Introductionmentioning

confidence: 99%

Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Shirazi

Heidari

Shirazi

et al. 2014

J Assist Reprod Genet

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“…This kind of stem cell is unipotent and responsible for the maintenance of spermatogenesis throughout the entire life of a male. They are the only germ line stem cells in adults and may choose self-renewal or generate a daughter cell committed to differentiation (15). These cells are biotechnologically important because they are the only cells in adult stem cell systems capable of transmitting genetic information to future generations (13).…”

Section: Introductionmentioning

confidence: 99%

Henna, An Antimicrobial Herbal Medicine With Less Negative Effect on Spermatogonial Stem Cell Culture

et al. 2016

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Background: Herbal extracts have recently received the greatest attention in the path of finding naturally occurring chemicals with antibacterial and therapeutic value; however, each type of herbal remedy may have its own side effects. Objectives:The aim of the current experiment was to study the antibacterial effect of myrtle, parsley, mint, henna and chamomile extracts on Escherichia coli and their effects on colony formation and survival of spermatogonial stem cells (SSCs). Materials and Methods: Spermatogonial stem cells were isolated by two-time enzymatic digestion from slaughterhouse origin ovine testis and plant extraction by deionized water. Comparisons between different treatments were performed using analysis of variance (ANOVA) followed by Duncan's multiple range tests. Results:The results showed that there was no significant difference between mint, henna and penicillin, on inhibition of Escherichia coli growth, however parsley, myrtle and chamomile were significantly different from penicillin (P < 0.05). The formation of SSC colonies was not affected by different herbal medicines. Myrtle and chamomile resulted in death of SSC colonies compared with the control, and the survival of spermatogonial and Sertoli cells were significantly affected by myrtle, mint, chamomile and parsley (P < 0.05). Conclusions:The results of these experiments provide evidence that henna by antibacterial activity had no detrimental affect on SSC and Sertoli cells and is a good candidate for substitution of antibiotics.

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Spermatogonial Stem Cells and Animal Transgenesis

Cited by 4 publications

References 99 publications

Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis

Effects of Extracellular Matrix Protein-derived Signaling on the Maintenance of the Undifferentiated State of Spermatogonial Stem Cells from Porcine Neonatal Testis

Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Henna, An Antimicrobial Herbal Medicine With Less Negative Effect on Spermatogonial Stem Cell Culture

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