Spermatotoxic, Spermagglutinating and Cytotoxic Activities of Guinea-Pig Autoantibodies to Sperm Autoantigen T

Toullet, F; Voisin, G A

doi:10.1530/jrf.0.0370299

Cited by 24 publications

(10 citation statements)

References 9 publications

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“…Spermagglutination and complement-dependent sperm immobilization were performed as described by Toullet & Voisin (1974) except that microplates with flat bottomed wells were used and the reactions were read in an inverted microscope.…”

Section: Methodsmentioning

confidence: 99%

Role of guinea-pig sperm autoantigens in sperm binding to the zona pellucida and oocyte penetration

Vera¹,

Guienne²,

Almeida³

et al. 1986

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In vitro, binding of acrosome-reacted spermatozoa to the zona pellucida of mature guinea-pig oocytes was inhibited by guinea-pig sperm anti-T IgG and antibodies. Anti-P IgG antibodies prevented oocyte penetration without interfering with spermzona binding. The fusion of acrosome-reacted spermatozoa with zona-free oocytes was prevented by anti-T IgG and it was diminished by anti-P IgG. In the same conditions anti-S antibodies had no effect in these in-vitro fertilization events. Immunization of female guinea-pigs with P antigen resulted in a significant decrease of the number of tubal cleaved eggs. T antigens were less clearly implicated in fertilization in vivo. This study provides evidence that well characterized autoantigenic molecules of guinea-pig spermatozoa are involved in fertilization events.

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Section: Methodsmentioning

confidence: 99%

Role of guinea-pig sperm autoantigens in sperm binding to the zona pellucida and oocyte penetration

Vera¹,

Guienne²,

Almeida³

et al. 1986

Reproduction

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“…Cytotoxicity was studied using trypan blue as a supravital stain [18]. Sera were used at titers in which no agglutination or immobilization was observed in iYtro.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Changes Induced in Human Spermatozoa by Subagglutinating Concentrations of Specific Antibodies

Huacuja

Delgado

Calzada

et al. 1978

Archives of Andrology

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The effects on sperm metabolism of subagglutinating concentrations of infertile female sera, containing antisperm antibody, were studied. Sera were selected by their differential agglutinating or immobilizing properties and by high titers of activity. Only the immobilizing antibodies were able to decrease sperm viability. Basal and succinate depending oxygen uptake, thus as progressive motility was decreased by sera producing mixed agglutination and more drastically by that producing immobilization independent of the presence of complement. The sera with head-to-head agglutination properties induced an increase in the above mentioned physiologic parameters. Tip of tail-to-tip of tail agglutinating sera having no effect on oxygen uptake were able to induce a significant increase in progressive motility. Conversion of IT-Glucose to 14C0, was decreased by sera that induced tip of tail-to-tip of tail agglutination and complementindependent immobilization. The sera which induced head-to-head agglutination and immobilization on the presence of complement, were without effect. None of the tested sera showed any activity over the conversion of 'T-Glucose to %Lactate.

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“…Sera in mice were pooled, inactivated by heating at 56~ for 30min, and assayed for spermagglutinating antibody by the tray agglutination test [30]. In brief, 501al of the sperm suspension prepared as described above (1 X 107/ml) was mixed with 50 lal of each of twofold serum dilutions using a 96-well microtiter plate and incubated at 37~ for 2h and at 4~ overnight.…”

Section: Spermagglutinating Antibodymentioning

confidence: 99%

A new mouse model for autoimmune orchitis

Fujii

Yokochi

Nakashima

et al. 1991

Med Microbiol Immunol

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Experimental autoimmune orchitis (EAO) was induced in SMA mice (H-2nondefined) by repeated injection at intervals of 30 days of syngeneic testis homogenate (TH) together with Klebsiella O3 lipopolysaccharide (KO3 LPS) as a potent adjuvant. EAO was not induced by repeated injection of TH alone or KO3 LPS alone. At 10 days after the secondary injection of TH + KO3 LPS, there was marked infiltration with neutrophils in the seminiferous tubules and in the interstitium of the testis accompanied by destruction of the architecture of the seminiferous tubules and hypospermatogenesis. At 20 days after the secondary injection, infiltration with neutrophils in these areas had been replaced mostly by mononuclear cells (lymphocytes, plasma cells, and macrophages). Histopathological changes of the testes became severer by further injections until the 10th injection. The EAO lesions in the terminal stage were characterized by complete destruction of the tubular architecture of the testis, fibrosis, and aspermatogenesis. Lesions in the terminal stage were not restored at all. Spermagglutinating antibody titers in the serum increased and delayed-type hypersensitivity against TH estimated by footpad swelling developed in mice injected repeatedly with TH + KO3 LPS. Using immunofluorescence, antibodies against acrosomal components and tail components of the spermatozoa were detected in serum of these mice.

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Spermatotoxic, Spermagglutinating and Cytotoxic Activities of Guinea-Pig Autoantibodies to Sperm Autoantigen T

Cited by 24 publications

References 9 publications

Role of guinea-pig sperm autoantigens in sperm binding to the zona pellucida and oocyte penetration

Role of guinea-pig sperm autoantigens in sperm binding to the zona pellucida and oocyte penetration

Metabolic Changes Induced in Human Spermatozoa by Subagglutinating Concentrations of Specific Antibodies

A new mouse model for autoimmune orchitis

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