Spermatozoa From the Isolated Cauda Epididymidis of Rabbits and Some Effects of Artificial Cryptorchidism

Glover, T. D.

doi:10.1530/jrf.0.0010121

Cited by 33 publications

(17 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two ligatures were placed around the distal portion of the body of the epididymis approximately 0-5 cm apart and the intervening segment was removed. Care was taken to avoid damage to associated blood vessels (Glover, 1960). There was no handling of the epididymis, except for the small portion that was ulti¬ mately removed as part of the operation.…”

Section: Methodsmentioning

confidence: 99%

“…They were then pooled, diluted to 10 ml with physiological saline and the total concentration of spermatozoa was determined. Motility was again checked after dilution, and smears stained with aqueous nigrosin-eosin (Glover, 1960) were prepared. From these smears, certain morphological features of the spermatozoa and the incidence of eosinophilic forms were examined.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Ageing of Rabbit Spermatozoa in the Male Tract and Its Effect on Fertility

Tesh¹,

Glover²

1969

Reproduction

Self Cite

View full text Add to dashboard Cite

The influence on fertility of the ageing of rabbit spermatozoa in the male tract has been investigated. To age the spermatozoa, the corpus epididymidis was ligated bilaterally so that the inflow of younger spermatozoa into the tail of the epididymis from higher levels of the duct was prevented. Ejaculated semen samples collected from the rabbits at different times after the operation were used for insemination. It was found that the fertilizing capacity of the spermatozoa was lost after 49 days. Losses before implantation increased progressively until 28-day\x=req-\ old spermatozoa were used, but post-implantation losses reached a peak with 35-day-old spermatozoa. After implantation, losses appeared to occur at progressively earlier stages of development as the age of the spermatozoa increased. In the strain ofrabbits used, several foetuses were seen to lack gall bladders and to have abnormal skull sutures following the insemination of does with aged spermatozoa.Throughout the experiment, the motility, morphology and reaction ofthe spermatozoa to staining with aqueous nigrosin\p=m-\eosin was examined. The significance and possible causes of adverse changes in ageing spermatozoa are discussed. INTRODUCTIONThe tail of the epididymis is a storage area for mature spermatozoa (Von Lanz

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Ageing of Rabbit Spermatozoa in the Male Tract and Its Effect on Fertility

Tesh¹,

Glover²

1969

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…These animals will be referred to hereafter as 'the low ligation group'. The operative procedure previously described (Glover, 1960) was followed, but in this instance a single suture was used and no portion of the epididymis was removed.…”

Section: Methodsmentioning

confidence: 99%

Some Reactions of Rabbit Spermatozoa to Ligation of the Epididymis

Gaddum¹,

Glover²

1965

Reproduction

Self Cite

View full text Add to dashboard Cite

“…In considering ways to approach such a question we have taken account of the fact that the ability of the cauda to maintain spermatozoa in a viable state is rapidly eliminated when androgenic support of the epithelium is withdrawn (Moore, 1928;Dyson & Orgebin-Crist, 1973; Orgebin-Crist, Danzo & Da vies, 1975) or, in scrotal mammals, when the cauda is raised to body temperature (Heller, 1929;Glover, 1960;Foldesy & Bedford, 1982). We have studied the effect of body temperature on the profile of proteins in the lumen of the cauda epididymidis, and compared this with the effects caused by androgen deprivation due to castration.…”

Section: Introductionmentioning

confidence: 99%