Spermatozoa stimulate prostaglandin synthesis and secretion in bovine oviductal epithelial cells

Kodithuwakku, Suranga P.; Miyamoto, Akio; Wijayagunawardane, Missaka P.B.

doi:10.1530/rep-06-0201

Cited by 50 publications

(58 citation statements)

References 33 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In an in vivo study in mice, the expression of ADM and prostaglandin was reported to be upregulated after the arrival of sperm in the oviduct (Fazeli et al 2004). A similar report showed that motile spermatozoa increased the secretion of prostaglandin and vasoactive peptides from the bovine oviduct in culture (Kodithuwakku et al 2002). These findings suggest that sperm may increase CBF via the release of ADM and other substances -a suggestion that is upheld by our results in this study.…”

Section: Discussionsupporting

confidence: 75%

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Liao

Ho²,

Tang³

2011

Reproduction

View full text Add to dashboard Cite

Our laboratory previously showed that oviduct produced the greatest amount of adrenomedullin (ADM) in the rat female reproductive tract. The aim of this study is to investigate the changes in ADM levels resulting from the contact between the sperm and the oviduct and the possible roles of ADM in ciliary beating and oviductal contractility. Oviducts from Sprague-Dawley rats removed at pre-and post-ovulatory stages were cut open longitudinally and treated with ADM and/or receptor blockers before ciliary beat frequency (CBF) was measured. The effects of sperm on ADM production and CBF in the oviduct were also determined. The contraction of the oviduct after treatment with ADM and receptor antagonists was measured using the organ-bath technique. The results showed that ADM increased the CBF in rat oviduct and this stimulating effect was blocked by the calcitonin-gene-related peptide (CGRP) receptor antagonist, hCGRP . CBF was lower in post-ovulatory than pre-ovulatory oviducts. The presence of sperm in the oviduct increased both the ADM level and CBF. ADM treatment was shown to inhibit the contractility of the oviduct by lowering the basal tone and decreasing the contraction amplitude. The ADM receptor antagonist, hADM , was effective in counteracting the relaxation effect of ADM in the oviduct. All in all, these results indicate that ADM may play a crucial role in transporting the gametes/embryos by regulating ciliary beating and muscular contraction.

show abstract

Section: Discussionsupporting

confidence: 75%

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Liao

Ho²,

Tang³

2011

Reproduction

View full text Add to dashboard Cite

show abstract

“…Additionally, SEM analysis demonstrated that PGE 2 significantly reduced the formation of NETs and suppressed the phagocytosis of sperm by PMNs, as observed in PMNs treated with the LH-stimulated BOEC supernatant. Moreover, sperm stimulate the secretion of PGE 2 by cultured BOECs (Kodithuwakku et al 2007). Thus, in addition to the action of LH, sperm may have a self-protective mechanism by the suppression of the phagocytic activity of PMNs through further enhancement of PGE 2 secretion in the microenvironment surrounding them.…”

Section: Sperm Phagocytosis By Pmns In Bovine Oviductmentioning

confidence: 99%

Bovine oviduct epithelial cells downregulate phagocytosis of sperm by neutrophils: prostaglandin E2 as a major physiological regulator

Marey¹,

Liu²,

Kowsar³

et al. 2014

Reproduction

Self Cite

View full text Add to dashboard Cite

This study aimed to investigate the presence of polymorphonuclear neutrophils (PMNs) in bovine oviduct fluid under physiological conditions and to determine the possible role of bovine oviduct epithelial cells (BOECs) in the regulation of the phagocytic activity of PMNs for sperm. During the pre-ovulatory stage, PMNs were identified in the bovine oviduct fluid in relatively constant numbers. In our experiments, PMNs were incubated for 4 h with the supernatant of cultured BOECs stimulated for 24 h by LH (10 ng/ml). Phagocytosis was then assayed by co-incubation of these PMNs with sperm treated to induce capacitation. The BOEC supernatant significantly suppressed sperm phagocytosis by PMNs, and the LH-stimulated BOEC supernatant further suppressed phagocytosis. Importantly, in the BOEC culture, LH stimulated the secretion of prostaglandin E 2 (PGE 2 ), which dose-dependently (10 K6 , 10 K7 , and 10 K8 M) suppressed sperm phagocytosis by PMNs. Furthermore, a PGEP 2 receptor antagonist significantly abrogated the inhibition of phagocytosis by the LH-stimulated BOEC supernatant. Additionally, using scanning electron microscopy, incubation of PMNs with either PGE 2 or LH-stimulated BOEC supernatant before phagocytosis was found to prevent the formation of DNA-based neutrophil extracellular traps for sperm entanglement. The results indicate that sperm are exposed to PMNs in the oviduct and PGE 2 released into the oviduct fluid after LH stimulation may play a major role in the suppression of the phagocytic activity of PMNs for sperm via interaction with EP 2 receptors. Thus, the bovine oviduct provides a PGE 2 -rich microenvironment to protect sperm from phagocytosis by PMNs, thereby supporting sperm survival in the oviduct.Free Japanese abstract A Japanese translation of this abstract is freely available at

show abstract

“…These culture systems have been used to obtain valuable information on gene and protein expression (Umezu et al 2003). They have also been used to gain a better understanding of the maternal interaction with gametes and embryos (Lee et al 2002;Fazeli et al 2004;Georgiou et al 2007;Kodithuwakku et al 2007;Ulbrich et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Effects of spermatozoa–oviductal cell coincubation time and oviductal cell age on spermatozoa–oviduct interactions

Aldarmahi

Elliott

Russell

et al. 2014

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Abstract. The oviduct plays a crucial role in sperm storage, maintenance of sperm viability and sperm transport to the site of fertilisation. The aim of the present study was to investigate the effects of oviductal cell culture passage number, oviductal cell age and spermatozoa-oviduct coincubation times on gene expression in oviductal cells. Immortalised oviductal epithelial cells (OPEC) obtained from two different cell passages (36 and 57) were subcultured three times with and without spermatozoa for 24 h (control group). In a second study, OPEC were cocultured with spermatozoa for different time intervals (0, 4, 12 and 24 h). Expression of adrenomedullin (ADM), heat shock 70 kDa protein 8 (HSPA8) and prostaglandin E synthase (PGES) in OPEC was measured by quantitative polymerase chain reaction. The expression of ADM and HSPA8 was decreased significantly in OPEC cells from Passage 57, particularly in the later subculture group. These effects on HSPA8, but not ADM, expression in OPEC were further altered after coculture with spermatozoa for 24 h. We also demonstrated that spermatozoa-oviduct coculture for 12 and 24 h resulted in significantly higher expression of ADM, HSPA8 and PGES in OPEC. Overall, the data suggest that the OPEC lose some of their properties as a result of oviductal cell aging and that there are spermatozoa-oviduct interactions leading to increased oviductal cell gene expression.

show abstract

Spermatozoa stimulate prostaglandin synthesis and secretion in bovine oviductal epithelial cells

Cited by 50 publications

References 33 publications

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Bovine oviduct epithelial cells downregulate phagocytosis of sperm by neutrophils: prostaglandin E2 as a major physiological regulator

Effects of spermatozoa–oviductal cell coincubation time and oviductal cell age on spermatozoa–oviduct interactions

Contact Info

Product

Resources

About