Spezifische Adsorbentien für das Peptidantibiotika synthetisierende Enzymsystem Gramicidin S Synthetase, 2. Kupplung niedermolekularer Spacer/Ligand‐Verbindungen mit reaktiven polymeren Trägern und Untersuchung der Adsorptionswechselwirkung dieser polymeren Produkte mit Gramcidin S Synthetase

Abstract

The inhibition constants (KI ) of aliphatic and aromatic amide derivatives of phenylalanine and prolin for GS1 and GS2 of the enzyme system gramicidin S synthetase were determined. These compounds act as competitive inhibitors. Some of these compounds (spacedfigand compounds) were reacted with epoxy-activated Sepharose 6B or with crosslinked poly(viny1 alcohol). The resulting -for GSI or GS2 more or less specific -adsorption materials, were used for the purification of these multi-enzyme systems.