Sphaerodes mycoparasitica biotrophic mycoparasite of 3-acetyldeoxynivalenol- and 15-acetyldeoxynivalenol-producing toxigenic Fusarium graminearum chemotypes

Vujanovic, Vladimir; Goh, Yit Kheng

doi:10.1111/j.1574-6968.2010.02201.x

Cited by 24 publications

(10 citation statements)

References 38 publications

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“…Direct interactions include mycoparasitism and hyperparasitism ( Table 1 ). They have been reported by Vujanovic and Goh [ 57 ] in a study evaluating the effectiveness of Sphaerodes mycoparasitica filamentous fungi against F. graminearum. The cited authors hypothesized that S. mycoparasitica absorbs aurofusarin from the attacked Fusarium cells by lysing the components of pathogenic cell membranes, such as chitin, and producing chitinase and chitosanase.…”

Section: Inhibition Of the Growth Of Fusarium Pmentioning

confidence: 76%

Microbial Inhibition of Fusarium Pathogens and Biological Modification of Trichothecenes in Cereal Grains

Wachowska

Packa

Wiwart

2017

Toxins

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Fungi of the genus Fusarium infect cereal crops during the growing season and cause head blight and other diseases. Their toxic secondary metabolites (mycotoxins) contaminate grains. Several dozen toxic compounds produced by fungal pathogens have been identified to date. Type B trichothecenes—deoxynivalenol, its acetyl derivatives and nivalenol (produced mainly by F. graminearum and F. culmorum)—are most commonly detected in cereal grains. “T-2 toxin” (produced by, among others, F. sporotrichioides) belongs to type-A trichothecenes which are more toxic than other trichothecenes. Antagonistic bacteria and fungi can affect pathogens of the genus Fusarium via different modes of action: direct (mycoparasitism or hyperparasitism), mixed-path (antibiotic secretion, production of lytic enzymes) and indirect (induction of host defense responses). Microbial modification of trichothecenes involves acetylation, deacetylation, oxidation, de-epoxidation, and epimerization, and it lowers the pathogenic potential of fungi of the genus Fusarium. Other modifing mechanisms described in the paper involve the physical adsorption of mycotoxins in bacterial cells and the conjugation of mycotoxins to glucose and other compounds in plant and fungal cells. The development of several patents supports the commercialization and wider application of microorganisms biodegrading mycotoxins in grains and, consequently, in feed additives.

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Section: Inhibition Of the Growth Of Fusarium Pmentioning

confidence: 76%

Microbial Inhibition of Fusarium Pathogens and Biological Modification of Trichothecenes in Cereal Grains

Wachowska

Packa

Wiwart

2017

Toxins

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“…SMCD 2220-01 shown mycoparasitic lifestyle and is promising biological control agent against mycotoxin producing Fusarium pathogens (Vujanovic and Goh 2010; Kim and Vujanovic 2016). In addition to the biocontrol effect, SMCD 2220-01 was effective in reducing AUR mycotoxin production in red pigmented Fusaria by down-regulating AUR gene expression (Vujanovic and Goh 2011, Vujanovic et al 2017). Although the mycoparasite showed efficacy in moderating DON, 3-ADON, 15-ADON, and production in Fusaria (Vujanovic and Chau 2012), the background mechanism of mycoparatism that occur at the molecular level is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica

Kim

Vujanovic

2017

AMB Expr

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A fungus Sphaerodes mycoparasitica SMCD 2220-01 is a host specific mycoparasite against plant pathogenic Fusarium species. Fusarium spp. are producing a plethora of mycotoxins including zearalenone (ZEN), deoxynivalenol (DON) and its acetylated derivatives, 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON). The SMCD 2220-01 strain substantially reduced DON, 3-ADON, 15-ADON, and ZEN production capacity in co-culture system. Degradation and detoxification of the pure mycotoxins were also achieved when exposed to SMCD 2220-01 in shake flasks. The thin layer chromatography (TLC) combined with high performance liquid chromatography–electrospray ionization-high resolution mass spectrometry (HPLC–ESI–HRMS) revealed that the amount of mycotoxins exposed to SMCD 2220-01 was considerably reduced compared to control. ZEN level was decreased by 97%, while zearalenone sulfate ([M−H+SO3]− at m/z 397.1052 C18H21O8S1) was detected as a metabolite of ZEN converted to less toxic molecule by the mycoparasite. Further, the mycoparasite appeared to degrade DON, 3-ADON, and 15-ADON by 89, 58, and 72%, respectively. The deoxynivalenol sulfate ([M−COCH3+SO3−CH2O]− at m/z 345.2300 C14H17O8S1) was detected as a less toxic metabolic product of DON and 3-ADON. These findings report the SMCD 2220-01 effectiveness to lower mycotoxins-producing capacities of Fusarium, degrade pure mycotoxins and transform them to less toxic metabolites, opening new opportunities for research and innovation for detoxification of mycotoxins.

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“…For this study, two fungal isolates from the Saskatchewan Microbial Collection and Database (SMCD), biocontrol SMCD 2220-01 strain of S. mycoparasitica Vujan. (2009) as a Fusarium specific mycoparasite [57,58], and SMCD 2243 F. graminearum Schwabe 3-ADON chemotype as a virulent plant pathogenic strain [13,59] were used. The fungi were grown on Potato Dextrose Agar (PDA) medium at 23 • C in darkness for 7 days and fresh cultures were used for in vitro assay.…”

Section: Plant and Fungal Materialsmentioning

confidence: 99%

Specific Mycoparasite-Fusarium Graminearum Molecular Signatures in Germinating Seeds Disabled Fusarium Head Blight Pathogen’s Infection

Kim

Lahlali

Karunakaran

et al. 2021

IJMS

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Advances in Infrared (IR) spectroscopies have entered a new era of research with applications in phytobiome, plant microbiome and health. Fusarium graminearum 3-ADON is the most aggressive mycotoxigenic chemotype causing Fusarium head blight (FHB) in cereals; while Sphaerodes mycoparasitica is the specific Fusarium mycoparasite with biotrophic lifestyle discovered in cereal seeds and roots. Fourier transform infrared (FTIR) spectroscopy analyses depicted shifts in the spectral peaks related to mycoparasitism mainly within the region of proteins, lipids, also indicating a link between carbohydrates and protein regions, involving potential phenolic compounds. Especially, S. mycoparasitica contributes to significant changes in lipid region 3050–2800 cm−1, while in the protein region, an increasing trend was observed for the peaks 1655–1638 cm−1 (amide I) and 1549–1548 cm−1 (amide II) with changes in indicative protein secondary structures. Besides, the peak extending on the region 1520–1500 cm−1 insinuates a presence of aromatic compounds in presence of mycoparasite on the F. graminearum root sample. Monitoring shift in improved seed germination, fungus-fungus interface through scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), and FTIR molecular signatures combined with principal component analysis (PCA) proved useful tools to detect an early mycoparasitism as a vital asset of the preventive biocontrol strategy against plant pathogens.

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Sphaerodes mycoparasitica biotrophic mycoparasite of 3-acetyldeoxynivalenol- and 15-acetyldeoxynivalenol-producing toxigenic Fusarium graminearum chemotypes

Cited by 24 publications

References 38 publications

Microbial Inhibition of Fusarium Pathogens and Biological Modification of Trichothecenes in Cereal Grains

Microbial Inhibition of Fusarium Pathogens and Biological Modification of Trichothecenes in Cereal Grains

Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica

Specific Mycoparasite-Fusarium Graminearum Molecular Signatures in Germinating Seeds Disabled Fusarium Head Blight Pathogen’s Infection

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