Sphingan Group of Exopolysaccharides (EPS)

Pollock, Thomas J.

doi:10.1002/3527600035.bpol5010

Cited by 9 publications

(8 citation statements)

References 40 publications

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“…Apparently, they are not effective on Sphingomonas sp. and their EPSs as might be expected from the physicochemical properties of the components involved (Balkwill w a t e r r e s e a r c h 4 5 ( 2 0 1 1 ) 4 0 5 e4 1 6 et al, Denner et al, 2001;Pollock, 2002). The study of the unique EPSs and glycosphingolipids of sphingomonads species might result in the development of more effective and directed cleaning methods to control biofouling.…”

Section: Chemical Treatment Is Not Cleaningmentioning

confidence: 99%

Effect of conventional chemical treatment on the microbial population in a biofouling layer of reverse osmosis systems

Bereschenko

Prummel

Euverink³

et al. 2011

Water Research

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The impact of conventional chemical treatment on initiation and spatiotemporal development of biofilms on reverse osmosis (RO) membranes was investigated in situ using flow cells placed in parallel with the RO system of a full-scale water treatment plant. The flow cells got the same feed (extensively pre-treated fresh surface water) and operational conditions (temperature, pressure and membrane flux) as the full-scale installation. With regular intervals both the full-scale RO membrane modules and the flow cells were cleaned using conventional chemical treatment. For comparison some flow cells were not cleaned. Sampling was done at different time periods of flow cell operation (i.e., 1, 5, 10 and 17 days and 1, 3, 6 and 12 months). The combination of molecular (FISH, DGGE, clone libraries and sequencing) and microscopic (field emission scanning electron, epifluorescence and confocal laser scanning microscopy) techniques made it possible to thoroughly analyze the abundance, composition and 3D architecture of the emerged microbial layers. The results suggest that chemical treatment facilitates initiation and subsequent maturation of biofilm structures on the RO membrane and feed-side spacer surfaces. Biofouling control might be possible only if the cleaning procedures are adapted to effectively remove the (dead) biomass from the RO modules after chemical treatment.

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Section: Chemical Treatment Is Not Cleaningmentioning

confidence: 99%

Effect of conventional chemical treatment on the microbial population in a biofouling layer of reverse osmosis systems

Bereschenko

Prummel

Euverink³

et al. 2011

Water Research

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“…However, a polysaccharide with both acidic residues has never been described before. The substitution of glucuronic acid and deoxyglucuronic acid in the third position might occur through the same mechanism as that for the change of rhamnose and mannose in the first position in S-88 or S-198, where the substituents are distributed statistically at a ratio of 1:1 (Pollock, 2002), or in welan, where a similar substitution in the side chain is observed. Possibly, the substitution of the hydroxyl group in position 2 from GlcA to 2dGlcA might be analogous to the separation mechanism of the hydroxyl group in position 6 from mannose to rhamnose.…”

Section: Matrix-assisted Laser Desorption/ionization-time-of-flight Mmentioning

confidence: 98%

“…They usually consist of a linear repeating tetrasaccharide containing rhamnose, glucose, mannose and glucuronic acid in differing proportions and frequency, and with distinct side chains, e.g., glycosidic bound glucose or rhamnose (Pollock 2002).…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of the exopolysaccharide PS-EDIV from Sphingomonas pituitosa strain DSM 13101

Schultheis

Dreger

Nimtz

et al. 2008

Appl Microbiol Biotechnol

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JournalApplied microbiology and biotechnology Members of the bacterial genus Sphingomonas are known to produce highly viscous polysaccharides in solution. The exopolysaccharide PS-EDIV was produced by Sphingomonas pituitosa strain DSM 13101, purified using centrifugation, and precipitation and its structure was elucidated by 1D and 2D NMR techniques and chemical microderivatization combined with various mass spectrometric techniques. The following repeating unit of the polysaccharide could be identified:In addition, the polysaccharide also contains acetyl and glyceryl groups whose exact positions were not determined. PS-EDIV is similar in structure to a known exopolysaccharide but differs in being the first bacterial polysaccharide in which two different glucuronic acids are combined. It caused a high viscosity of the culture broth after cultivation for 48 h, although a gelation was not observed.

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“…Moreover, they are used in pharmaceutical/biomedical [19,20], cosmetic [21], chemical [22,23] and food industries [24,25]. …”

Section: Introductionmentioning

confidence: 99%

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

et al. 2013

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BackgroundMarine environments are the widest fonts of biodiversity representing a resource of both unexploited or unknown microorganisms and new substances having potential applications. Among microbial products, exopolysaccharides (EPS) have many physiological functions and practical applications. Since EPS production by many bacteria is too scarce for practical use and only few species are known for their high levels of production, the search of new high EPS producers is of paramount importance. Many marine bacteria, that produce EPS to cope with strong environmental stress, could be potentially exploited at the industrial level.ResultsA novel bacterium, strain BM39, previously isolated from sediments collected in the Tyrrhenian Sea, was selected for its production of very high levels of EPS. BM39 was affiliated to Pantoea sp. (Enterobacteriaceae) by 16S rRNA gene sequencing and biochemical tests. According to the phylogenetic tree, this strain, being quite far from the closest known Pantoea species (96% identity with P. agglomerans and P. ananatis) could belong to a new species. EPS production was fast (maximum of ca. 21 g/L in 24 h on glucose medium) and mainly obtained during the exponential growth. Preliminary characterization, carried out by thin layer and gel filtration chromatography, showed that the EPS, being a glucose homopolymer with MW of ca. 830 kDa, appeared to be different from those of other bacteria of same genus. The bacterium showed a typical slightly halophilic behavior growing optimally at NaCl 40 ‰ (growing range 0-100 ‰). Flow cytometry studies indicated that good cell survival was maintained for 24 h at 120 ‰. Survival decreased dramatically with the increase of salinity being only 1 h at 280 ‰. The biochemical characterization, carried out with the Biolog system, showed that MB39 had a rather limited metabolic capacity. Its ability, rather lower than that of P. agglomerans, was almost only confined to the metabolization of simple sugars and their derivatives. Few alcohols, organic acids and nitrogen compounds were partially used too.ConclusionsStrain BM39, probably belonging to a new species, due to its remarkable EPS production, comparable to those of known industrial bacterial producers, could be suggested as a new microorganism for industrial applications.

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Sphingan Group of Exopolysaccharides (EPS)

Abstract: Introduction Historical Outline Chemical Structures Structural Variations Analytical Methods Occurrence Physiology Properties Biosynthesis Synthesis of Sugar‐nucleotide Substrates … Show more

Cited by 9 publications

References 40 publications

Effect of conventional chemical treatment on the microbial population in a biofouling layer of reverse osmosis systems

Effect of conventional chemical treatment on the microbial population in a biofouling layer of reverse osmosis systems

Structural characterization of the exopolysaccharide PS-EDIV from Sphingomonas pituitosa strain DSM 13101

Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide

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