Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk

Abstract

A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2, was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH (summed feature 3). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids. The DNA G+C content of the strain was 38.9 mol%. The 16S rRNA gene sequence analysis indicated that strain YK2 was a member of the genus Sphingobacterium and closely related to Sphingobacterium alimentarium and Sphingobacterium composti with pair-wise sequence similarity of 98.3 and 97.9 %, respectively. The sequence similarity to other members of the genus Sphingobacterium was between 92.6 to 96.3 %. Phylogenetic analysis showed that strain YK2 clustered with Sphingobacterium alimentarium and together clustered with Sphingobacterium composti. DNA-DNA hybridization of strain YK2 with Sphingobacterium alimentarium WCC 4521 and Sphingobacterium composti T5-12 showed a relatedness of only 38 and 54 %, respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain YK2 represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bovisgrunnientis sp. nov. is proposed. The type strain of Sphingobacterium bovisgrunnientis sp. nov. is YK2 (=MTCC 12631=KCTC 52685=JCM 31951).

Microbial Populations of Fresh and Cold Stored Donkey Milk by High-Throughput Sequencing Provide Indication for A Correct Management of This High-Value Product

“…Besides, Sphingobacterium spp. has been isolated from different habitats, including raw cow and yak milk [37][38][39]. Massilia spp.…”

Sphingobacterium cavernae sp. nov., a novel bacterium isolated from soil sampled at Tiandong Cave

“…Members of this genus are Gram-stain-negative, non-motile and rod-shaped and its chemotaxonomic features include MK-7 as predominant isoprenoid quinone and iso-C 15 : 0 , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and iso-C 17 : 0 3OH as major fatty acids. Species of the genus Sphingobacterium mainly were isolated from leaf tissues, soil, lake, compost, insect, nodule surface of soybean, and milk, such as Sphingobacterium haloxyli [5], Sphingobacterium corticibacter [6], Sphingobacterium tabacisoli [7], Sphingobacterium yamdrokense [8], Sphingobacterium composti [9], Sphingobacterium griseoflavum [10], Sphingobacterium yanglingense [11], Sphingobacterium bovisgrunnientis [12] and Sphingobacterium alimentarium [13]. A bacterium, designated strain 5.0403-2 T , was isolated from a cave soil sample collected from Tiandong Cave in Guizhou Province during a study of microbial diversity in a karst district.…”

Sphingobacterium chungjuense sp. nov., isolated from a freshwater lake

“…[1] based on the description of three species ( Sphingobacterium spiritivorum , Sphingobacterium multivorum and Sphingobacterium mizutae ) harbouring high amounts of cellular sphingophospholipids. As of May 2020, the genus comprises 55 species with validly published names (https://lpsn.dsmz.de), which have been isolated from a variety of habitats such as soil [2–4], plant [5–7], clinical specimens [1, 8], milk [9, 10], lake water [11], sludge [12], food-waste compost [13], insect [14] and lichen [15]. In particular habitats, such as a gut of fall armyworm [16] or an enrichment of apple pomace [17], the genus showed high abundance, accounting for 17–40 % of the total bacterial population.…”

“…dsmz. de), which have been isolated from a variety of habitats such as soil [2][3][4], plant [5][6][7], clinical specimens [1,8], milk [9,10], lake water [11], sludge [12], food-waste compost [13], insect [14] and lichen [15]. In particular habitats, such as a gut of fall armyworm [16] or an enrichment of apple pomace [17], the genus showed high abundance, accounting for 17-40 % of the total bacterial population.…”