Sphingobacterium griseoflavum sp. nov., isolated from the insect Teleogryllus occipitalis living in deserted cropland

Abstract

A Gram-stain-negative, aerobic, rod-shaped, non-motile bacterial strain, designated SCU-B140T , was isolated from the insect Teleogryllus occipitalis. Phylogenetic analysis on the basis of 16S rRNA gene sequence showed that strain SCU-B140 T belonged to the genus Sphingobacterium. Sphingobacterium bambusae KCTC 22814 T (97.87 %) was identified as the most closely related phylogenetic neighbour of strain SCU-B140T . The novel strain was able to grow at salt concentrations of 0-4 % (w/v), at temperatures of 10-40 8C, and at a pH of 6.0-9.0. The major cellular fatty acids were iso-C 15 : 0 , summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 v7c), C 16 : 0 , C 16 : 0 3-OH, C 18 : 0 and C 14 : 0 . The major polar lipids consisted of phosphatidylethanolamine, three unknown aminophospholipids, an unknown glycolipid and three unknown polar lipids. MK-7 was the major isoprenoid quinone. The DNA G+C content was 41.2 mol%. The DNA-DNA relatedness value between SCU-B140 T and S. bambusae KCTC 22814T was found to be 30.15 %. According to these results, strain SCU-B140 T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium griseoflavum sp. nov. is proposed. The type strain is SCU-B140 T (5KCTC 42158 T 5CGMCC

Sphingobacterium cavernae sp. nov., a novel bacterium isolated from soil sampled at Tiandong Cave

“…Members of this genus are Gram-stain-negative, non-motile and rod-shaped and its chemotaxonomic features include MK-7 as predominant isoprenoid quinone and iso-C 15 : 0 , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and iso-C 17 : 0 3OH as major fatty acids. Species of the genus Sphingobacterium mainly were isolated from leaf tissues, soil, lake, compost, insect, nodule surface of soybean, and milk, such as Sphingobacterium haloxyli [5], Sphingobacterium corticibacter [6], Sphingobacterium tabacisoli [7], Sphingobacterium yamdrokense [8], Sphingobacterium composti [9], Sphingobacterium griseoflavum [10], Sphingobacterium yanglingense [11], Sphingobacterium bovisgrunnientis [12] and Sphingobacterium alimentarium [13]. A bacterium, designated strain 5.0403-2 T , was isolated from a cave soil sample collected from Tiandong Cave in Guizhou Province during a study of microbial diversity in a karst district.…”

Sphingomonas corticis sp. nov., and Sphingobacterium corticibacterium sp. nov., from bark canker

“…At the time of writing, the genus comprises 55 species with validly published names (https://lpsn.dsmz.de/genus/sphingobacterium). Sphingobacterium species have been found from various environment including fresh water, compost, activated sludge, clinical specimen, raw milk, plants, insects and different kinds of soil [12–24]. Some species of the genus have the ability to degrade cellulose, petroleum and cypermethrin [22, 25, 26].…”

Sphingobacterium chungjuense sp. nov., isolated from a freshwater lake

“…dsmz. de), which have been isolated from a variety of habitats such as soil [2][3][4], plant [5][6][7], clinical specimens [1,8], milk [9,10], lake water [11], sludge [12], food-waste compost [13], insect [14] and lichen [15]. In particular habitats, such as a gut of fall armyworm [16] or an enrichment of apple pomace [17], the genus showed high abundance, accounting for 17-40 % of the total bacterial population.…”