Spiegelmers as potential receptors for cTnI diagnostics

Szeitner, Zsuzsanna; Doleschall, Anna; Varga, Marina; Keltai, Katalin; Révész, Katalin; Gyurcsányi, Róbert E.; Mészáros, Tamás

doi:10.1039/c7ay01777d

Cited by 2 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, we further developed and successfully applied the domain method to produce spiegelmers for an N-terminally localized peptide motif of cardiac troponin I (cTnI), one of the generally accepted standard biomarkers of acute coronary syndrome (ACS) [ 14 ]. In the latter study, these spiegelmers were leveraged for developing an antibody-spiegelmer-composed homogenous sandwich assay that was suitable for selective detection of cTnI [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection

Tolnai

András

Szeitner

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Two subunits of the ternary troponin complex, I and C, have cardiac muscle specific isoforms, and hence could be applied as highly-selective markers of acute coronary syndrome. We aimed at paving the way for the development of a robust cardiac troponin I-detecting sandwich assay by replacing antibodies with nuclease resistant aptamer analogues, so-called spiegelmers. To complement the previously generated spiegelmers that were specific for the N-terminus of cTnI, spiegelmers were selected for an amino acid stretch in the proximity of the C-terminal part of the protein by using a D-amino acid composed peptide. Following the selection, the oligonucleotides were screened by filter binding assay, and surface plasmon resonance analysis of the most auspicious candidates demonstrated that this approach could provide spiegelmers with subnanomolar dissociation constant. To demonstrate if the selected spiegelmers are functional and suitable for cTnI detection in a sandwich type arrangement, AlphaLisa technology was leveraged and the obtained results demonstrated that spiegelmers with different epitope selectivity are suitable for specific detection of cTnI protein even in human plasma containing samples. These results suggest that spiegelmers could be considered in the development of the next generation cTnI monitoring assays.

show abstract

Section: Introductionmentioning

confidence: 99%

Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection

Tolnai

András

Szeitner

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

Aptamers Targeting Cardiac Biomarkers as an Analytical Tool for the Diagnostics of Cardiovascular Diseases: A Review

et al. 2022

View full text Add to dashboard Cite

The detection of cardiac biomarkers is used for diagnostics, prognostics, and the risk assessment of cardiovascular diseases. The analysis of cardiac biomarkers is routinely performed with high-sensitivity immunological assays. Aptamers offer an attractive alternative to antibodies for analytical applications but, to date, are not widely practically implemented in diagnostics and medicinal research. This review summarizes the information on the most common cardiac biomarkers and the current state of aptamer research regarding these biomarkers. Aptamers as an analytical tool are well established for troponin I, troponin T, myoglobin, and C-reactive protein. For the rest of the considered cardiac biomarkers, the isolation of novel aptamers or more detailed characterization of the known aptamers are required. More attention should be addressed to the development of dual-aptamer sandwich detection assays and to the studies of aptamer sensing in alternative biological fluids. The universalization of aptamer-based biomarker detection platforms and the integration of aptamer-based sensing to clinical studies are demanded for the practical implementation of aptamers to routine diagnostics. Nevertheless, the wide usage of aptamers for the diagnostics of cardiovascular diseases is promising for the future, with respect to both point-of-care and laboratory testing.

show abstract

Spiegelmers as potential receptors for cTnI diagnostics

Abstract: We demonstrate the first application of a nuclease resistant aptamer, the so-called Spiegelmer, in a sandwich-type affinity assay by quantitative assessment of cardiac Troponin I (cTnI) in blood serum samples.

Cited by 2 publications

References 18 publications

Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection

Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection

Aptamers Targeting Cardiac Biomarkers as an Analytical Tool for the Diagnostics of Cardiovascular Diseases: A Review

Contact Info

Product

Resources

About