Spinal and intestinal levels of substance P, calcitonin gene-related peptide and vasoactive intestinal polypeptide following perendoscopic injection of formalin in rat colonic wall

Miampamba, Marcel; Chéry‐Croze, Sylviane; Chayvialle, J. A.

doi:10.1016/0143-4179(92)90057-4

Cited by 22 publications

(17 citation statements)

References 25 publications

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“…CGRP was found to be reduced in the inflamed bowel in animal models of chemicallyinduced colitis (Eysselein et al 1991;Miampamba et al 1992;Miampamba and Sharkey 1998). Moreover, tissues from patients with Crohn's disease and ulcerative colitis also showed a decrease in the number and density of CGRP-positive nerve fibers in the colonic mucosa (Koch et al 1987;Eysselein et al 1992).…”

Section: Discussionmentioning

confidence: 94%

Alterations in the distal colon innervation in Winnie mouse model of spontaneous chronic colitis

et al. 2015

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The gastrointestinal tract is innervated by extrinsic sympathetic, parasympathetic and sensory nerve fibers as well as by intrinsic fibers from the neurons in myenteric and submucosal ganglia embedded into the gastrointestinal wall. Morphological and functional studies of intestinal innervation in animal models are important for understanding the pathophysiology of inflammatory bowel disease (IBD). The recently established Winnie mouse model of spontaneous chronic colitis caused by a point mutation in the Muc2 mucin gene develops inflammation due to a primary epithelial defect. Winnie mice display symptoms of diarrhea, ulcerations and rectal bleeding similar to those in IBD. In this study, we investigated myenteric neurons, noradrenergic, cholinergic and sensory nerve fibers in the distal colon of Winnie (Win/Win) mice compared to C57/BL6 and heterozygote littermates (Win/Wt) using histological and immunohistochemical methods. All Win/Win mice used in this study had inflammation with signs of mucosal damage, goblet cell loss, thickening of muscle and mucosal layers, and increased CD45-immunoreactivity in the distal colon. The density of sensory, cholinergic and noradrenergic fibers innervating the myenteric plexus, muscle and mucosa significantly decreased in the distal colon of Win/Win mice compared to C57/BL6 and Win/Wt mice, while the total number of myenteric neurons as well as subpopulations of cholinergic and nitrergic neurons remained unchanged. In conclusion, changes in the colon morphology and innervation found in Winnie mice have multiple similarities with changes observed in patients with ulcerative colitis.

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Section: Discussionmentioning

confidence: 94%

Alterations in the distal colon innervation in Winnie mouse model of spontaneous chronic colitis

et al. 2015

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“…Analysis of changes in the number of VIP-ergic nerve elements is difficult due to the different phasing of the state of inflammation of the intestine. The earlier fall in the number of VIP-positive nerve fibres in the large intestinal wall of the rat, and next a later increase in their number have been observed [33]. The data given above indicate that the problem dealing with changes in the quantity of VIP in nerve elements supplying the large intestinal wall in various pathological states has not been definitively resolved; in this connection, there is also a lack of data relating to changes in the number of VIP-ergic nerve elements in the human intestine undergoing neoplasmal processes.…”

Section: Discussionmentioning

confidence: 97%

Changes in vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y-ergic structures of the enteric nervous system in the carcinoma of the human large intestine.

Godlewski

Łakomy²

2010

Folia Histochem Cytobiol.

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This investigation was aimed at immunohistochemical analysis of potential changes in the enteric nervous system caused by cancer of the large intestine. In this purpose, neurons and nerve fibers of intestinal plexuses containing neuropeptides: vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating polypeptide (PACAP) and neuropeptide Y (NPY), in pathologically changed part of the large intestine were microscpically observed and compared. Samples were taken from patients operated due to cancer of the sigmoid colon and rectum. The number of neurons and density of nerve fibres containing neuropeptides found in sections with cancer tissues were compared to those observed in sections from the uninvolved intestinal wall. Changes relating to reductions in the number of NPY-ergic neurons and density of nerve fibres in submucous and myenteric plexuses in the sections with cancer tissues (pathological sections) were statistically significant. A statistically similar presence of VIP-ergic and PACAP-ergic neurons in the submucosal and myenteric plexuses was observed in both the pathological and control sections. On the other hand, in the pathological sections, VIP-ergic nerve fibres in the myenteric plexuses and PACAP-ergic nerve fibres in the submucosal and myenteric plexuses were found to be less dense. Analysis revealed changes in pathologically affected part of the large intestine may caused disruption of proper intestinal function. Observed changes in the neural elements which are responsible for relaxation of the intestine may suggest dysfunction in the innervation of this part of the colon.

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“…Recent studies have shown that bee venom treatment can induce a significant antiinflammatory response mediated by inhibition of inflammatory mediators, similar to what is achieved with the administration of non-steroidal anti-inflammatory drugs (Miampamba et al, 1992). In experimental rheumatoid arthritis, bee venom treatment significantly decreased the expression of inflammation-related cytokines such as cyclooxygenase-2 (COX-2), phospholipase A2 (PLA2), tumor necrosis factor-α (TNF-α), interleukin (IL)-1, IL-6, nitric oxide (NO) and reactive oxygen species (ROS) (Son et al, 2007) via NF-κB (Kim et al, 2013).…”

Section: Discussionmentioning

confidence: 93%

“…The α 2 -adrenoceptor agonist has been shown to induce antinociceptive effect in the experimental model of formalin-induced colitis in rats and reduce visceral hypersensitivity in clinical settings (Lima-Júnior et al, 2006;Miampamba et al, 1992). Therefore, a possible involvement of α 2 -adrenoceptors in the antinociceptive effect of AMV, using the antagonist yohimbine was investigated.…”

Section: Discussionmentioning

confidence: 99%

Study of visceral antinociceptive potential of bee Apis mellifera venom

Costa¹,

Campos²,

Abdon³

et al. 2014

Afr. J. Pharm. Pharmacol.

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Pain is one of the most common reasons for patients to seek medical care. Bee Apis mellifera venom (AMV) has traditionally been used to treat inflammatory diseases and the alleviation of pain. Herein, we aimed to investigate the visceral antinociceptive potential of A. mellifera bee venom and its possible mechanism of action. Acetic acid-induced writhing assay was used in mice to determine the degree of visceral antinociception. Visceral antinociceptive activity was expressed as the reduction in the number of abdominal constrictions. Mice received an intraperitoneal injection of acetic acid after administration of AMV (0.08 or 0.8 mg/kg; intraperitoneally (i.p.)). In mechanistic studies, separate experiments were realized to examine the role of α2-receptors, nitric oxide, calcium channels, K + ATP channel activation, TRPV1 and opioid receptors on the visceral antinociceptive effect of AMV (0.8 mg/kg), using appropriate antagonists, yohimbine (2 mg/kg), L-NG-Nitroarginine methyl ester (L-NAME, 10 mg/kg), verapamil (5 mg/kg), glibenclamide (5 mg/kg), ruthenium red (3 mg/kg) or naloxone (2 mg/kg). AMV presented visceral antinociceptive activity in both doses tested (0.08 and 0.8 mg/Kg). Visceral antinociceptive effect of AMV was resistant to all the antagonists used. Mice showed no significant alterations in locomotion frequency, indicating that the observed antinociception is not a consequence of motor abnormality. Although AMV efficient diminished the acetic acid-evoked pain-related behavior, its mechanism is unclear from this study and future studies are needed to verify how the venom exerts its antinociceptive action.

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Spinal and intestinal levels of substance P, calcitonin gene-related peptide and vasoactive intestinal polypeptide following perendoscopic injection of formalin in rat colonic wall

Cited by 22 publications

References 25 publications

Alterations in the distal colon innervation in Winnie mouse model of spontaneous chronic colitis

Alterations in the distal colon innervation in Winnie mouse model of spontaneous chronic colitis

Changes in vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y-ergic structures of the enteric nervous system in the carcinoma of the human large intestine.

Study of visceral antinociceptive potential of bee Apis mellifera venom

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