Background: Overuse of electronic products and decrease in levels of physical activity may lead to an increased incidence of spinal health problems in college students. This study aims to 1) identify the incidence of spinal health problems in college students, 2) to explore the key postural factors in development of spinal musculoskeletal dysfunction and scoliosis, and 3) to clarify new strategies for early identification and prevention of scoliosis. Methods: A cross-sectional screening by static posture assessment, selective functional movement assessment (SFMA) and Adam’s Forward Bend Test,was conducted to 306 college students from October to December 2019. Descriptive analysis, Spearman correlation analysis and binomial logistic regression analysis were performed on the collected data. Results: Static postural assessment was completed among 306 college students, 53% of students’ external auditory meatus and acromioclavicular joint were not in the same gravitational line, 45% had forward-head posture, 42% had uneven shoulders. When performing SFMA, we found spinal musculoskeletal dysfunctions, including abnormalities of multi-segment spine flexion (32%), cervical rotation to right (18%), cervical rotation to left (16%), etc. In Adam’s Forward Bend Test, 10.78% had a positive result, which suggested structural or functional scoliosis. Correlation analysis showed that the top three postural factors related to spinal musculoskeletal dysfunctions included uneven shoulders, forward-head, position of thorax and pelvis rotated. And top three postural factors related to scoliosis were spinous processes line deviating from midline, winged scapula and head rotation off midline. Logistic regression analysis displayed that there are two high risk factors for scoliosis, including external auditory meatus and acromioclavicular joint not in the same vertical line (OR = 6.314), and deviation of spinous processes line (OR = 12.743). Conclusions: Poor posture, spinal musculoskeletal dysfunction and scoliosis affect a significant proportion of college students. While their spinal musculoskeletal dysfunction and scoliosis are closely related to poor posture.