Spinning elastic beads: a route for simultaneous measurements of the shear modulus and the interfacial energy of soft materials

Carbonaro, Alessandro; Chagua-Encarnacion, Kennedy-Nexon; Charles, Carole-Ann; Phou, Ty; Ligoure, Christian; Mora, Serge; Truzzolillo, Domenico

doi:10.1039/d0sm01024c

Cited by 6 publications

(7 citation statements)

References 48 publications

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“…This means that the relationship between the applied external stress and the deformation of the sensor involves both the shear stress modulus µ d and the surface tension γ c . For small deformations, it has been shown that one can take into account this elasto-capillary contribution by introducing an effective elastic constant µ e (Carbonaro et al, 2020): with K is a dimensionless constant of order unity. We thus performed an in situ calibration on a sensor embedded in a tissue, in order to directly measure µ e .…”

Section: Resultsmentioning

confidence: 99%

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

Souchaud

Boutillon

Charron

et al. 2021

Preprint

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To investigate the role of mechanical constraints in morphogenesis and development, we develop a pipeline of techniques based on incompressible elastic sensors. These techniques combine the advantages of incompressible liquid droplets, which have been used as precise in situ shear stress sensors, and of elastic compressible beads, which are easier to tune and to use. Droplets of a polydimethylsiloxane (PDMS) mix, made fluorescent through specific covalent binding to a rhodamin dye, are produced by a microfluidics device. The elastomer rigidity after polymerization is adjusted to the tissue rigidity. Its mechanical properties are carefully calibrated in situ, for a sensor embedded in a cell aggregate and submitted to uniaxial compression. The local shear stress tensor is retrieved from the sensor shape, accurately reconstructed through an active contour method. In vitro, within cell aggregates, and in vivo, in the prechordal plate of the Zebrafish embryo during gastrulation, our pipeline of techniques demonstrates its efficiency to directly measure the three dimensional shear stress repartition within a tissue, and its time evolution.

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Section: Resultsmentioning

confidence: 99%

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

Souchaud

Boutillon

Charron

et al. 2021

Preprint

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show abstract

“…tension γc. For small deformations, it has been shown that one can take into account this elasto-capillary contribution by introducing an effective elastic constant µe (Carbonaro et al, 2020):…”

Section: In Situ Calibration In Aggregatesmentioning

confidence: 99%

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

et al. 2022

View full text Add to dashboard Cite

To investigate the role of mechanical constraints in morphogenesis and development, we develop a pipeline of techniques based on incompressible elastic sensors. These techniques combine the advantages of incompressible liquid droplets, which have been used as precise in situ shear stress sensors, and of elastic compressible beads, which are easier to tune and to use. Droplets of a polydimethylsiloxane (PDMS) mix, made fluorescent through specific covalent binding to a rhodamin dye, are produced by a microfluidics device. The elastomer rigidity after polymerization is adjusted to the tissue rigidity. Its mechanical properties are carefully calibrated in situ, for a sensor embedded in a cell aggregate submitted to uniaxial compression. Thelocal shear stress tensor is retrieved from the sensor shape, accurately reconstructed through an active contour method. In vitro, within cell aggregates, and in vivo, in the prechordal plate of the Zebrafish embryo during gastrulation,our pipeline of techniques demonstrates its efficiency to directly measure the three dimensional shear stress repartition within a tissue.

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“…The dominant approach to tackle this fundamental question consists in investigating surface elastic properties. 12,[15][16][17][18] For instance, surface topography measurements of a stretched patterned silicone gel revealed an elastic surface, where surface stresses increase with surface deformations. 15 This result hints towards a role of the crosslinked polymeric network in the surface constitutive behavior of silicone gels.…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, deformation measures of a spinning hydrogel bead evidenced constant surface stresses, independent of surface deformations, akin to the solvent surface tension. 18 In its simplest form, the most common description for the surface mechanics of soft solids relates surface stresses r s (in N m À1 ) to surface strains e s and free energy W s (in J m À2 or N m À1 ),…”

Section: Introductionmentioning

confidence: 99%

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From a distance: Shuttleworth revisited

Heyden,

Bain

2024

Soft Matter

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Spinning elastic beads: a route for simultaneous measurements of the shear modulus and the interfacial energy of soft materials

Abstract: Large deformations of soft elastic beads spinning at high angular velocity in a denser background fluid are investigated theoretically, numerically, and experimentally using millimeter-size polyacrylamide hydrogel particles introduced in a...

Cited by 6 publications

References 48 publications

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

From a distance: Shuttleworth revisited

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