SPION based magnetic PLGA nanofibers for neural differentiation of mesenchymal stem cells

Abstract

Recently, magnetic platforms have been widely investigated in diagnostic, therapeutic and research applications due to certain properties, such as cell and tissue tracking and imaging, thermal therapy and being dirigible. In this study, the incorporation of magnetic nanoparticles (MNPs) in nanofibers has been proposed to combine the advantages of both nanofibers and MNPs to induce neural differentiation of mesenchymal stem cells. Magnetic poly (lactic-co-glycolic acid) nanofibers (containing 0%, 5% and 10% SPION) were fabricated and utilized as the matrix for the differentiation of mesenchymal stem cells (MSCs). Morphological, magnetic and mechanical properties were analyzed using FESEM, VSM and tensile test, respectively. The expression of neural markers (TUJ-1, NSE, MAP-2) was assessed quantitative and qualitatively utilizing RT-PCR and immunocytochemistry. Results confirmed the incorporation of MNPs in nanofibrous scaffold, presenting a saturation magnetization of 9.73 emu g−1. Also, with increase in magnetic particle concentration (0%–10%), tensile strength increased from 4.08 to 5.85 MPa, whereas the percentage of elongation decreased. TUJ-1 expression was 3.8 and 1.8 fold for 10% and 5% magnetic scaffold (versus non-magnetic scaffold) respectively, and the expression of NSE was 6.3 and 1.2-fold for 10% and 5%, respectively. Consequently, it seems that incorporation of magnetic biomaterial can promote the neural differentiation of MSCs, during which the augmentation of super paramagnetic iron oxide concentration from 0% to 10% accelerates the neural differentiation process.

Magnetic Force Microscopy and Nanoindentation on 3D Printed Magnetic Scaffolds for Neuronal Cell Growth

“…In this context, the inclusion of metallic material results in composites characterized by increased stiffness or even dynamic modifiability through the influence of an external magnetic field. This phenomenon was observed in MNPs embedded in gelatin nanofibers, where the application of an external magnetic field induces fiber alignment, thereby enhancing the material’s stiffness. In the presence of these aligned fibers, stem cells (ADSC cells) underwent differentiation into osteocytes, whereas in the absence of fiber alignment, the cells differentiated into adipocytes …”

“…23 Magnetic poly(lactic-co-glycolic acid) nanofibers successfully differentiated mesenchymal stem cells into neurons. 24 Chitosan-MNP composites mediated the differentiation of neural stem cells (NSC) into neurons during nerve repair. 25 This enhancement is attributed to the inclusion of MNPs, which finely adjust the physicochemical properties of the material.…”

“…Implants based on magnetic alginate, hyaluronic acid, and collagen hydrogels promoted the regeneration of axon densities similar to a rat sciatic nerve . Magnetic poly­(lactic- co -glycolic acid) nanofibers successfully differentiated mesenchymal stem cells into neurons . Chitosan-MNP composites mediated the differentiation of neural stem cells (NSC) into neurons during nerve repair .…”

The Recent Applications of PLGA-Based Nanostructures for Ischemic Stroke

“…Mohammadalizadeh et al prepared SPION-based magnetic PLGA nanofibers and used them as substrates for MSC differentiation. The magnetic biomaterials could stimulate MSCs to differentiate into neurons, and increasing the content of superparamagnetic ferric oxide from 0 to 10% sped up the procedure [ 139 ]. PLGA-PEG nanofibers can create the optimal microenvironment, encourage the development of networks around neurons, and enhance synaptogenesis and inter-neuronal communication [ 140 ].…”

Comparison of chitosan and SLN nano-delivery systems for antibacterial effect of cinnamon (Cinnamomum verum) oil against MDR K pneumoniae and E coli

“…Solid lipid nanoparticles (SLNs) are particular lipid-based biocompatible nanocarriers with a nanotechnology based delivery system and a diameter size range of 10-1000 nm [24,25]. As colloidal drug delivery systems, SLNs are physiologically well tolerated due to their matrix consisting of solid lipids, and the cytotoxicity of this system is low [26].…”