Spiral microchannels on a CD for DNA hybridizations

Peng, Xingyue; Li, Paul C. H.; Yu, Hua‐Zhong; Parameswaran, M.; Chou, Wa Lok Jacky

doi:10.1016/j.snb.2007.05.038

Cited by 42 publications

(43 citation statements)

References 18 publications

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“…More advanced microchannel arrangements can be designed to carry analytes from multiple sources across multiple nanhole array sensor elements simultaneously for assay-type analysis [18]. Further, chip designs that rely on active fluid transport rather than basic capillary action could be incorporated [19]; however, we choose a passive mechanism such that this integrated sensor will require less external powering, no extra control components, and have a lower production cost.…”

Section: System Architecturementioning

confidence: 99%

Large-Area Low-Cost Flexible Plastic Nanohole Arrays for Integrated Bio-Chemical Sensing

et al. 2013

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Detection of plasmonic resonance peak shifts of nano-structured metamaterials is a promising method for sensing bio-chemical binding events. Although the concept is widely demonstrated in the laboratory environment using surface nanostructures machined at low-throughput and high-costs, practical solutions for high-volume production of an integrated sensing device are very limited. We present a concept of an integrated architecture that combines a thin layer of plasmonic nanohole sensing arrays, organic light-emitting diode illumination source, and microfluidic chip, for point-of-care, field, or lab applications. We discuss the fabrication of the sensor components. In particular, we present the improved fabrication of master nano-structure replication stamps, and demonstrate outstanding results for producing singular sheets or scale up to roll-toroll embossing of nanohole arrays on a 2000 ft production roll. We further demonstrate that nanohole arrays embossed on flexible polyethylene terephthalate plastic sheets, when coated with 100 nm thin Au metal film, are capable of generating average plasmonic resonance shifts of 180 nm refractive index unit. Hence, we report the extraordinary transmission and plasmonic resonance shifts of nanohole arrays fabricated on roll-to-roll plastic sheets for the very first time. Our results show that the embossed nano-structures on plastic are suitable as sensor elements in our proposed integrated sensor architecture, and a promising technology for low-cost disposable applications.

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Section: System Architecturementioning

confidence: 99%

Large-Area Low-Cost Flexible Plastic Nanohole Arrays for Integrated Bio-Chemical Sensing

et al. 2013

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“…30,31 The excitation and emission wavelengths are 633 and 670 nm, respectively. The photomultiplier tube voltage was set to 600 V. The scanned image was analyzed by IM-AGEQUANT 5.2 software.…”

Section: E Probe Line Printing Sample Hybridization and Results Reamentioning

confidence: 99%

Gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device

Wang

2010

Biomicrofluidics

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Two simple gold nanoparticle ͑GNP͒-based DNA analysis methods using a microfluidic device are presented. In the first method, probe DNA molecules are immobilized on the surface of a self-assembled submonolayer of GNPs. The hybridization efficiency of the target oligonulceotides was improved due to nanoscale spacing between probe molecules. In the second method, target DNA molecules, oligonulceotides or polymerase chain reaction ͑PCR͒ amplicons, are first bound to GNPs and then hybridized to the immobilized probe DNA on a glass slide. With the aid of GNPs, we have successfully discriminated, at room temperature, between two PCR amplicons ͑derived from closely related fungal pathogens, Botrytis cinerea and Botrytis squamosa͒ with one base-pair difference. DNA analysis on the microfluidic chip avoids the use of large sample volumes, and only a small amount of oligonucelotides ͑8 fmol͒ or PCR products ͑3 ng͒, was needed in the experiment. The whole procedure was accomplished at room temperature in 1 h, and apparatus for high temperature stringency was not required.

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“…In order to circumvent the difficulties in device design and fabrication, there has been increasing interest in developing the flow-through microfluidic devices for DNA hybridization [8][9][10][11][12][13][14][15]. For example, some microfluidic devices drove fluid to continuously flow within the hybridization channel using hydrodynamic pumping [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…For example, some microfluidic devices drove fluid to continuously flow within the hybridization channel using hydrodynamic pumping [8][9][10]. The CD-based microfluidic devices were also presented [11][12][13][14][15]. They utilized centrifugal force to pump fluid and manipulated the transfer of liquids by the control of rotation speed of the CD or laser irradiated ferrowax microvalves [16,17].…”

Section: Introductionmentioning

confidence: 99%

Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device

Dong

Qin

et al. 2009

Analytica Chimica Acta

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Spiral microchannels on a CD for DNA hybridizations

Cited by 42 publications

References 18 publications

Large-Area Low-Cost Flexible Plastic Nanohole Arrays for Integrated Bio-Chemical Sensing

Large-Area Low-Cost Flexible Plastic Nanohole Arrays for Integrated Bio-Chemical Sensing

Gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device

Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device

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