A number of studies point to the relationship between spirituality and religiosity with health and well-being. However, there are few instruments to evaluate these aspects among adolescents. The objective of this study was to verify the psychometric properties of the Spirituality, Religiousness and Personal Beliefs module, part of the World Health Organization’s Quality of Life Instrument (WHOQOL-SRPB) by analyzing evidence of validity and reliability among Brazilian adolescents. The participants were 1,248 Brazilian adolescents, between 12 and 18 years of age (M = 15.09, SD = 1.77), 57.3% of whom were girls. Confirmatory factor analyses indicated that the eight-factor composition presents good fit indexes, and adequate reliability. Multi-group analyses indicated that there is factorial invariance by gender and age. It is concluded that the instrument presents evidence of validity and reliability with Brazilian adolescents. However, further studies are recommended especially considering the principal components analyses.