Abstract. Morphology of the nematode Viguiera dicrurusi Gupta, 1960 harboured by Dicrurus macrocercus albirictus (Hodgson) (Passseriformes: Dicruridae) from Baruipara in 24-Pargonas (South) district, West Bengal, India was studied by light and scanning electron microscopy (SEM). This represents the first study of V. dicrurusi using SEM. Scanning electron micrographs provided detailed information about the nature of pseudolabial plates, number and shape of teeth, dentate nature of striae, and the relative position of vulva, anus and phasmid opening in female. A detailed morphometrical comparison of this species with Viguiera viduae Chabaud, 1960 The genus Viguiera Seurat, 1913 now includes 20 species; of these 9 have been described from the Indian Subcontinent. The cephalic structures are important taxonomic features of this genus, and have been inadequately described in some of the Indian species, which include Viguiera dicrurusi Gupta, 1960, Viguiera bhujangai Jehan, 1972 and Viguiera adsimilisai Sood et Kalia, 1978. These three species and Viguiera majumdari De, 1979 have been described from similar or related hosts of the same zoogeographical region and are very close in their various body measurements. Viguiera indica Jehan, 1972 is based on a single male specimen. The cephalic structure of V. majumdari is quite different from V. dicrurusi, and in V. indica the left spicule is much longer than in other Indian congeners; therefore, they are considered distinct species. Unfortunately, types of V. bhujangai and V. adsimilisai are not available for restudy. The place of deposition of the type specimens of V. bhujangai was not mentioned in the literature and no reply in this regard was received from the institution where the author worked. The types of V. adsimilisai are lost. Viguiera bhujangai and V. adsimilisai are thus considered species inquirendae.New data are added on the morphology of V. dicrurusi below, based on studies with the light microscope (LM) and scanning electron microscope (SEM).
MATERIALS AND METHODSFor the present study eight black drongoes [Dicrurus macrocercus albirictus (Hodgson)], collected from Baruipara, 24-Pargonas (South) district, West Bengal, India were examined for nematode infection. Five out of the eight hosts were found to be infected. Sixteen male and nineteen female nematodes were recovered from under the horny layer of the gizzard of the infected hosts. The worms were washed thoroughly by shaking in 0.85% saline. Eight males and eleven females were fixed in hot 4% formaldehyde and were cleared in 5% glycerine for LM study.For SEM study, eight males and eight females were fixed in 2.5% glutaraldehyde in 0.2 M sodium cacodylate buffer at pH 7.0 for 4 hours. After primary fixation, the anterior and poste-rior ends of the worms were cut off by using a sharp razor blade to promote better fixation and minimize shrinkage. The cut ends were postfixed for 12 hours in 2% osmium tetroxide in the same buffer, dehydrated in ascending series of ethanol, transferred to 100% isoamyl acetate...