Split aptamer regulated CRISPR/Cas12a biosensor for 17β-estradiol through a gap-enhanced Raman tags based lateral ﬂow strategy

Li, Qing; Li, Xiaobo; Zhou, Pengyou; Chen, Rui; Xiao, Rui; Pang, Yuanfeng

doi:10.1016/j.bios.2022.114548

Cited by 32 publications

(19 citation statements)

References 38 publications

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“…In the case of this approach, it has several unparalleled advantages for AFB 1 detection: (1) Triple signal amplification mediated by the Cas enzyme, RCA, and HRP endows the method with high sensitivity. (2) Previous CRISPR/Cas12a-mediated aptasensors mainly include a fluorophore quencher-based reporter or nanoparticle/tag molecule-based reporter , that either suffer from relatively low sensitivity and photobleaching or were limited by the stability of the functional material. In comparison to these reported approaches, our enzyme reporter-based strategy has a relatively high sensitivity with simple signal amplification and excellent stability.…”

Section: Resultsmentioning

confidence: 99%

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

Wang

Wei

Ruan

et al. 2023

J. Agric. Food Chem.

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Herein, we report a CRISPR/Cas12a-assisted chemiluminescence sensor for aflatoxin B 1 (AFB 1 ) detection based on functional nucleic-acid-mediated target recognition and in-pipet rolling circle amplification-mediated signal amplification. In this sensor, we performed rolling circle amplification on the inside of the pipet to enrich horseradish peroxidase (pipet-poly-HRP). When AFB 1 is present, it interacts with functional nucleic acids and results in the release of the activator. The activator is designed to activate the CRISPR/Cas12a system, which cleaves the pipet-poly-HRP to liberate HRP. The freed HRP can then be measured by chemiluminescence to quantify AFB 1 . This CRISPR/Cas12a-assisted chemiluminescence sensor enables facile, highly sensitive, and specific detection of AFB 1 , with a linear range from 50 pg/mL to 100 ng/mL and a detection limit of 5.2 pg/mL. Furthermore, it exhibits satisfactory recovery and has successfully challenged AFB 1 detection in cereal samples. The proposed sensor offers a novel rapid screening approach that holds great promise for food security monitoring.

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Section: Resultsmentioning

confidence: 99%

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

Wang

Wei

Ruan

et al. 2023

J. Agric. Food Chem.

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show abstract

“…In addition, this can be completed by healthier lifestyles, a clean environment and smart web sensors. Therefore, researchers are now implementing intelligent 5th generation biosensors to protect precious life from these dangerous diseases ( Lei et al, 2019 ; Li et al, 2022 ; Novoselov et al, 2004 ; Pan et al, 2022 ; Patel et al, 2016 ; Solanki et al, 2011 ; Verma and Bhardwaj, 2015 ).…”

Section: State-of-the-art 5th Generation Biosensors: Towards Hospital...mentioning

confidence: 99%

Towards hospital-on-chip supported by 2D MXenes-based 5th generation intelligent biosensors

Chaudhary

Khanna

Awan

et al. 2023

Biosensors and Bioelectronics

114

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“…CaT-SMelor aTF [88] fDNAs (two ATP aptamers) ATP [82] HRP-amplification [83] Lateral flow biosensor 17β-estradiol [86] DNAzyme (fDNA) Na + [82] Metal-labeled CRISPR/Cas12a Kanamycin [90] Cas14a Enzyme strand Pb 2+ [85] An elemental probe-based CRISPR/Cas14 AMP [91]…”

Section: Cas12amentioning

confidence: 99%

CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

Chen

et al. 2023

Nano Res.

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Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, cis -cleavage and nonspecific trans -cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.

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Split aptamer regulated CRISPR/Cas12a biosensor for 17β-estradiol through a gap-enhanced Raman tags based lateral ﬂow strategy

Cited by 32 publications

References 38 publications

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

Towards hospital-on-chip supported by 2D MXenes-based 5th generation intelligent biosensors

CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

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Split aptamer regulated CRISPR/Cas12a biosensor for 17β-estradiol through a gap-enhanced Raman tags based lateral ﬂow strategy

Cited by 32 publications

References 38 publications

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B1 Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B1 Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

Towards hospital-on-chip supported by 2D MXenes-based 5th generation intelligent biosensors

CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

Contact Info

Product

Resources

About

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification

CRISPR/Cas12a-Assisted Chemiluminescence Sensor for Aflatoxin B₁ Detection in Cereal Based on Functional Nucleic Acid and In-Pipet Rolling Circle Amplification