Spoilage potential characterization ofShewanellaandPseudomonasisolated from spoiled large yellow croaker (Pseudosciaena crocea)

Ge, Yu; Zhu, Junli; Ye, X.; Yu, Yang

doi:10.1111/lam.12687

Cited by 62 publications

(34 citation statements)

References 25 publications

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“…are not improved with the addition of TMAO. TMAO reduction has been reported in species ofAlteromonas and Pseudomonas (Ringø et al, 1984; Barrett and Kwan, 1985;Ge et al, 2017). However, we noticed that TMAO didn't promote the growth for most of the isolates from the two genera in our study.…”

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confidence: 71%

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Yin

Zhang

et al. 2018

J. Ocean. Limnol.

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Trimethylamine N-oxide (TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from different genera harbor high hydrostatic pressure (HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from fish which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affiliated to 23 genera of Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at different depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas,

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confidence: 71%

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Yin

Zhang

et al. 2018

J. Ocean. Limnol.

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“…Gram‐negative proteolytic psychrotrophic bacteria, mainly Shewanella and Pseudomonas, have been identified as the dominant spoilage organism during iced storage of fish due to their ability to produce volatile sulphides, amines and trimethylamine (Gram and Melchiorsen ; Ge et al . ). Shewanella species can form biofilm under diverse environments, and Shewanella putrefaciens is usually associated with biofilm formation and adhere to stainless steel surfaces inducing corrosion of processing steel surface (Bagge et al .…”

Section: Introductionmentioning

confidence: 97%

“…; Ge et al . ). However, limited information is available on psychrotrophic spoilage bacteria in biofilm influenced by temperature.…”

Section: Introductionmentioning

confidence: 97%

“…In a previous study, two spoilage bacteria, S. baltica and P. fluorescens , were isolated and identified from chilled large yellow croaker ( Pseudosciaena crocea ) (Ge et al . ). The aim of this study was to characterized the biofilm produced by mono‐ and dual‐species of S. baltica and P. fluorescens during the different incubation temperature and time, and to elucidate the interactive behaviour of dual‐species biofilm development.…”

Section: Introductionmentioning

confidence: 97%

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Competitive interaction on dual‐species biofilm formation by spoilage bacteria, Shewanella baltica and Pseudomonas fluorescens

et al. 2019

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Aims: This study aims to characterize the biofilm produced by mono-and dual-species of Shewanella baltica and Pseudomonas fluorescens as fish spoilers at the different incubation temperature, and to elucidate the interactive behaviour of dual-species biofilm development. Methods and Results: The mono-and dual-species biofilm formation and adhesion characteristics of S. baltica and P. fluorescens were evaluated by using crystal violet staining, scanning electron microscopy and confocal laser scanning microscopy. Results showed that P. fluorescens had significantly higher biofilm biomass and polysaccharides production than S. baltica, and two isolates reached the maximum biofilm biomass during the early stationary phase. Lower biomass and polysaccharides in dual-species biofilms were observed compared to mono-species of P. fluorescens. Meanwhile, S. baltica and P. fluorescens formed fragile and viscous pellicles with different spatial architectures respectively. In dual-species pellicle few large microcolonies were dominated by P. fluorescens. Compared to mono-species of PF07, adherent cell population and biofilm thickness at the developing phase significantly decreased, and biofilm-forming cycle prolonged in the dual-species biofilms. Biofilm formation and adhesion of mono-and dual-species at 4 or 15°C were significantly higher than at 30°C during the same phase. The culture supernatant extracts of the two spoilage strains greatly inhibited biofilm development to each other. Conclusions: Shewanella baltica and P. fluorescens had different biofilm and pellicle characteristics, and the inhibitory development on dual-species biofilm was associated with the competitive interaction by the two psychrotrophic spoilage bacteria. Significance and Impact of the Study: This work contributes to a better understanding of interactive behaviour of multispecies biofilm communities by psychrotrophic spoilage bacteria at low temperature, which could contribute to further control contamination of spoilage organism during the preservation and processing of aquatic products.

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“…The most frequently detected Pseudomonas sp. in chilled food is Pseudomonas fluorescens , which contributes predominantly to food spoilage during storage of many refrigerated aquatic products (Sillankorva et al ; Oh et al ; Ge et al ). In addition, patients showed severe clinical presentations when they received blood and blood products polluted by P. fluorescens , such as sepsis, septic shock and intravascular coagulation (Sun et al ).…”

Section: Introductionmentioning

confidence: 99%

Virtual screening for quorum‐sensing inhibitors of Pseudomonas fluorescens P07 from a food‐derived compound database

Ding

2019

J Appl Microbiol

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Aims: Pseudomonas fluorescens are important psychrotrophic food spoilage bacteria that are frequently detected in dairy, meat and aquatic products. Quorum sensing (QS) is an intercellular communication and gene regulation mechanism that enables bacteria to monitor their cell densities and regulate a variety of physiological processes. Hence, targeting the bacterial QS system might be a feasible approach to improve food quality and safety by regulating the spoilage caused by P. fluorescens. Methods and Results: In this study, we screened a food-derived threedimensional (3D) compound database to search for potential QS inhibitors (QSIs) with higher security. The 3D structures of LuxI-and LuxR-type proteins of P. fluorescens P07 were used as targets to screen for QSIs. A total of 25 compounds with high docking scores were tested for their anti-QS activities by indicator strains. The results show that 19 compounds possessed anti-QS activities. Among them, (+)-catechin had the strongest anti-QS activity. The results show that (+)-catechin significantly inhibited the production of extracellular enzymes, swimming motility, biofilm formation, acyl-homoserine lactones and extracellular polymeric substances (EPSs) of P. fluorescens P07. The inhibitory mechanism of (+)-catechin on the QS system of P. fluorescens P07 was discussed in the context of molecular docking analysis and real-time quantitative PCR (RT-qPCR). Conclusions: Virtual screening was useful in finding novel QSIs with high security of P. fluorescens P07 from a food-derived 3D compound database. The high hit rate suggested that foods are rich sources of QSIs, and have great potential for exploration. Significance and Impact of the Study: The modelled LuxI-and LuxR-type proteins could be used as targets to discover P. fluorescens P07 QSIs. (+)catechin, (À)-epicatechin, propyl gallate, hesperidin and lycopene which were identified as potent QSIs, and may be applied in food preservation and biofilm elimination.

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Spoilage potential characterization ofShewanellaandPseudomonasisolated from spoiled large yellow croaker (Pseudosciaena crocea)

Cited by 62 publications

References 25 publications

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Competitive interaction on dual‐species biofilm formation by spoilage bacteria, Shewanella baltica and Pseudomonas fluorescens

Virtual screening for quorum‐sensing inhibitors of Pseudomonas fluorescens P07 from a food‐derived compound database

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