Sponge-associated fungi from a mangrove habitat in Indonesia: species composition, antimicrobial activity, enzyme screening and bioactive profiling

Sibero, Mada Triandala; Igarashi, Yasuhiro; Radjasa, Ocky Karna; Sabdono, Agus; Trianto, Agus; Zilda, Dewi Seswita; Wijaya, Yusuf Jati

doi:10.1007/s40071-019-0227-8

Cited by 28 publications

(31 citation statements)

References 42 publications

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“…Both pigment types have significant antimicrobial and antioxidant properties [ 6 , 19 , 43 , 44 ]. Yellow pigments of haloarchaea are compounds such as 4,4’-diaponeurosporene, zeaxanthin, or lutein with applications in food colorants, visual acuity, and antibacterial activity against multidrug-resistant organisms [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Ubiquitousness of Haloferax and Carotenoid Producing Genes in Arabian Sea Coastal Biosystems of India

et al. 2021

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This study presents a comparative analysis of halophiles from the global open sea and coastal biosystems through shotgun metagenomes (n = 209) retrieved from public repositories. The open sea was significantly enriched with Prochlorococcus and Candidatus pelagibacter. Meanwhile, coastal biosystems were dominated by Marinobacter and Alcanivorax. Halophilic archaea Haloarcula and Haloquandratum, predominant in the coastal biosystem, were significantly (p < 0.05) enriched in coastal biosystems compared to the open sea. Analysis of whole genomes (n = 23,540), retrieved from EzBioCloud, detected crtI in 64.66% of genomes, while cruF was observed in 1.69% Bacteria and 40.75% Archaea. We further confirmed the viability and carotenoid pigment production by pure culture isolation (n = 1351) of extreme halophiles from sediments (n = 410 × 3) sampling at the Arabian coastline of India. All red-pigmented isolates were represented exclusively by Haloferax, resistant to saturated NaCl (6 M), and had >60% G + C content. Multidrug resistance to tetracycline, gentamicin, ampicillin, and chloramphenicol were also observed. Our study showed that coastal biosystems could be more suited for bioprospection of halophiles rather than the open sea.

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Section: Discussionmentioning

confidence: 99%

Ubiquitousness of Haloferax and Carotenoid Producing Genes in Arabian Sea Coastal Biosystems of India

et al. 2021

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“…Chrysoporthe sp. exhibited strong antibacterial activity against E. coli and S. aureus (Nirma et al, 2015); and Fusarium species produced metabolites with activities against microbial pathogens (Sibero et al, 2019). Besides, from F. larvarum, an antifungal compound active against C. albicans and a wide range of pathogenic fungi was isolated, called Parafungin (Harvey et al, 2015).…”

Section: Fungal Strain Code Labmicramentioning

confidence: 99%

Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

Oliveira

Santiago

Katak

et al. 2021

RSD

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Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis. In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.

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“…Our previous research also showed that spongeassociated fungi have several bioactivities such as anti-S. aureus and E. coli (Wittriansyah et al 2016), antibacterial produced by sponge Agelas sp. associated fungi Fusarium solani , and Sibero (2019) has also reported that sponge-associated fungi from a mangrove habitat were producing anti-microbial activities.…”

Section: Introductionmentioning

confidence: 99%

Biological activity of sponge-associated fungi from Karimunjawa Islands, Indonesia against pathogenic Streptococcus pneumoniae

et al. 2019

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Abstract. Cristianawati O, Sabdaningsih A, Becking LE, Khoeri MM, Nuryadi H, Sabdono A, Trianto A, Radjasa OK. 2019. Biological activity of sponge-associated fungi from Karimunjawa Islands, Indonesia against pathogenic Streptococcus pneumoniae. Biodiversitas 20: 2143-2150. Threats of drug-resistant Streptococcus pneumoniae and the urgent need for new antibiotics require prompt and sustained action for discovering bioactive compounds. This study aimed to isolate sponge-associated fungi as a candidate for sources of drugs. The sponge-associated fungi were screened against the MDR S. pneumoniae. The Deoxyribonucleic Acid (DNA) of fungal isolates that showed the most active was generated by using universal primers ITS1 and ITS4. While the sponge host was identified based on the molecular and by slide section. Out of twenty-nine fungal isolates from 13 marine sponges, S.06.2 isolate produced secondary metabolite that inhibiting the growth of MDR S. pneumoniae. Molecular identification based on the ITS region revealed that the active fungal isolate was closely related to Curvularia lunata, with a 99% similarity. The C. lunata S.06.2 was isolated from sponge Cinachyrella australiensis. Thus, C. lunata S.06.2_ LC315806 can serve as a fruitful strategy for the discovery of novel antibiotics for the treatment of MDR S. pneumoniae.

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Sponge-associated fungi from a mangrove habitat in Indonesia: species composition, antimicrobial activity, enzyme screening and bioactive profiling

Cited by 28 publications

References 42 publications

Ubiquitousness of Haloferax and Carotenoid Producing Genes in Arabian Sea Coastal Biosystems of India

Ubiquitousness of Haloferax and Carotenoid Producing Genes in Arabian Sea Coastal Biosystems of India

Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

Biological activity of sponge-associated fungi from Karimunjawa Islands, Indonesia against pathogenic Streptococcus pneumoniae

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