Spontaneous liberation of intracellular polyhydroxybutyrate granules in Escherichia coli

Jung, Il Lae; Phyo, Ki Heon; Kim, Kug Chan; Park, Hyo Kook; Kim, In Gyu

doi:10.1016/j.resmic.2005.04.004

Cited by 40 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A clean recovery of P(3HB) have also been envisaged by the use of supercritical CO 2 [54], but this method appears to be still expensive in comparison with other methods. Recently, new methods like spontaneous liberation of P(3HB) [67], dissolved air flotation [65], or air classification [64] are being investigated and are probably promised to have much more success. Improvement of these new extraction and purification methods should lead to an optimal recovery of P(3HB), with a high purity and recovery level at a low production cost.…”

Section: Resultsmentioning

confidence: 99%

“…This method was developed in 2005 by Jung et al They used a low cell inoculum and 2× LB medium containing 21% glucose. The results showed that the recombinant MG1655 accumulated P(3HB) up to an efficiency of 99% from the glucose supplied, and up to 80% of cells spontaneously secreted P(3HB) granules outside of the cells [67]. These experiments indicated that the use of a simpler purification process could be possible, such as from one-step centrifugation/washing with distilled water [67].…”

Section: Spontaneous Liberationmentioning

confidence: 91%

“…By using a new cultivation method, involving recombinant E. coli harboring Alcaligenes phbCAB genes, spontaneous liberation of intracellualar P(3HB) granules can be obtained [67]. This method was developed in 2005 by Jung et al They used a low cell inoculum and 2× LB medium containing 21% glucose.…”

Section: Spontaneous Liberationmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and purification of bacterial poly(3-hydroxyalkanoates)

Jacquel

Wei

et al. 2008

Biochemical Engineering Journal

349

185

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Spontaneous Liberationmentioning

confidence: 91%

See 1 more Smart Citation

Isolation and purification of bacterial poly(3-hydroxyalkanoates)

Jacquel

Wei

et al. 2008

Biochemical Engineering Journal

349

185

View full text Add to dashboard Cite

“…7 Developing effi cient microbial disruption technologies for PHA recovery and purifi cation based on cell autolysis or using low-cost lytic enzymes would substitute for current chemical separation methods, improve production economics and reduce environmental impact. 8,9 Apart from biofuels and biodegradable plastics, cereals could also be used for the production of a range of platform chemicals. Koutinas et al 10 discussed the feasibility of the production of a wide range of chemicals from wheat based on the production yield from natural microorganisms, wheat availability and eff ect of feedstock cost.…”

Section: Cereal-based Biorefi Neriesmentioning

confidence: 99%

The biochemurgist –Bioconversion of agricultural raw materials for chemical production

Koutinas

Wang

Webb

2007

Biofuels Bioprod Bioref

110

View full text Add to dashboard Cite

Current cereal-and oilseed-based processes for the production of chemicals should be restructured into advanced biorefi ning strategies in order to reduce environmental impact, improve overall economics and meet market/societal needs. Next generation biorefi neries should utilize all grain or oilseed components as well as any associated residues for the production of a spectrum of products ranging from biofuels and biodegradable plastics to commodity, platform and speciality chemicals. Generic biorefi ning concepts are proposed in the case of wheat and rapeseed. Glycerol produced as by-product from biodiesel production could be upgraded into an important raw material for the production of various chemicals through microbial bioconversions or enzymatic biotransformations.

show abstract

“…Over the past 20 years, however, signiWcant advances in the upstream fermentation and downstream PHA recovery technologies [28,36,42] have been made to improve the eYciency and economy of PHA manufacturing [56]. Production of PHAs from low-value feedstocks such as forest-related process streams, has potential to signiWcantly reduce the substrate expenditure component of the overall manufacturing cost (up to 50%) [15,44].…”

Section: Economic Considerations For Commercial Pha Productionmentioning

confidence: 99%

Polyhydroxyalkanoate copolymers from forest biomass

Keenan

Nakas

Tanenbaum

2006

J IND MICROBIOL BIOTECHNOL

View full text Add to dashboard Cite

The potential for the use of woody biomass in poly-beta-hydroxyalkanoate (PHA) biosynthesis is reviewed. Based on previously cited work indicating incorporation of xylose or levulinic acid (LA) into PHAs by several bacterial strains, we have initiated a study for exploring bioconversion of forest resources to technically relevant copolymers. Initially, PHA was synthesized in shake-flask cultures of Burkholderia cepacia grown on 2.2% (w/v) xylose, periodically amended with varying concentrations of levulinic acid [0.07-0.67% (w/v)]. Yields of poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) [P(3HB-co-3HV)] from 1.3 to 4.2 g/l were obtained and could be modulated to contain from 1.0 to 61 mol% 3-hydroxyvalerate (3HV), as determined by 1H and 13C NMR analyses. No evidence for either the 3HB or 4HV monomers was found. Characterization of these P(3HB-co-3HV) samples, which ranged in molecular mass (viscometric, Mv) from 511-919 kDa, by differential scanning calorimetry and thermogravimetric analyses (TGA) provided data which were in agreement for previously reported P(3HB-co-3HV) copolymers. For these samples, it was noted that melting temperature (Tm) and glass transition temperature (Tg) decreased as a function of 3HVcontent, with Tm demonstrating a pseudoeutectic profile as a function of mol% 3HV content. In order to extend these findings to the use of hemicellulosic process streams as an inexpensive carbon source, a detoxification procedure involving sequential overliming and activated charcoal treatments was developed. Two such detoxified process hydrolysates (NREL CF: aspen and CESF: maple) were each fermented with appropriate LA supplementation. For the NREL CF hydrolysate-based cultures amended with 0.25-0.5% LA, P(3HB-co-3HV) yields, PHA contents (PHA as percent of dry biomass), and mol% 3HV compositions of 2.0 g/l, 40% (w/w), and 16-52 mol% were obtained, respectively. Similarly, the CESF hydrolysate-based shake-flask cultures yielded 1.6 g/l PHA, 39% (w/w) PHA contents, and 4-67 mol% 3HV compositions. These data are comparable to copolymer yields and cellular contents reported for hexose plus levulinic acid-based shake-flask cultures, as reported using Alcaligenes eutrophus and Pseudomonas putida. However, our findings presage a conceivable alternative, forestry-based biorefinery approach for the production of value-added biodegradable PHA polymers. Specifically, this review describes the current and potential utilization of lignocellulosic process streams as platform precursors to PHA polymers including hemicellulosic hydrolysates, residual cellulose-derived levulinic acid, tall oil fatty acids (Kraft pulping residual), and lignin-derived aromatics.

show abstract

Spontaneous liberation of intracellular polyhydroxybutyrate granules in Escherichia coli

Cited by 40 publications

References 23 publications

Isolation and purification of bacterial poly(3-hydroxyalkanoates)

Isolation and purification of bacterial poly(3-hydroxyalkanoates)

The biochemurgist –Bioconversion of agricultural raw materials for chemical production

Polyhydroxyalkanoate copolymers from forest biomass

Contact Info

Product

Resources

About