Spontaneous periodic synchronized bursting during formation of mature patterns of connections in cortical cultures

Kamioka, Hayato; Maeda, Eisaku; Jimbo, Yasuhiko; Robinson, Hugh P. C.; Kawana, Akio

doi:10.1016/s0304-3940(96)12448-4

Cited by 332 publications

(273 citation statements)

References 19 publications

Supporting

Mentioning

237

Contrasting

Unclassified

Order By: Relevance

“…Using recordings from single networks over the course of 6 weeks, the present study extends these observations and demonstrates that neuronal avalanches are a robust form of neuronal synchronization that occurs throughout postnatal development. Large changes in activity levels have been reported previously for organotypic slice cultures during postnatal maturation (Maeda, Robinson et al, 1995;Kamioka, Maeda et al, 1996;Corner, van Pelt et al, 2002;Johnson and Buonomano, 2007) and are also found in our study. Importantly, we show that despite these changes in activity levels, the organization of synchronization in the form of neuronal avalanches remains constant for many weeks even in single cultures.…”

Section: The Organization Of Spontaneous Synchronized Activity In Orgsupporting

confidence: 91%

“…Gopal and Gross, 1996;Kamioka, Maeda et al, 1996;Tateno, Kawana et al, 2002). A direct comparison of findings in the current study with those reported in dissociated cultures, however, encounters a number of difficulties.…”

Section: The Organization Of Spontaneous Synchronized Activity In Orgmentioning

confidence: 60%

“…In the cortex, this hallmark of spontaneous activity has been reported since the earliest culture success in vitro (Crain, 1966;Calvet, 1974) and naturally occurs in all isolated cortex preparations thus studied, e.g. in organotypic cultures (Plenz and Aertsen, 1996a;Plenz and Aertsen, 1996b;Gorba, Klostermann et al, 1999;Klostermann and Wahle, 1999;Baker, Corner et al, 2006), dissociated cultures (Maeda, Robinson et al, 1995;Gopal and Gross, 1996;Kamioka, Maeda et al, 1996;Canepari, Bove et al, 1997;Jimbo, Kawana et al, 2000;Segev, Shapira et al, 2001;van Pelt, Corner et al, 2004;Eytan and Marom, 2006), acute cortex slices (Sanchez-Vives and McCormick, 2000), and acutely isolated cortical slabs in vivo (Steriade, Nuñez et al, 1993;Timofeev, Grenier et al, 2000). Quantification of these Correspondence: Dr. Dietmar Plenz, Section on Critical Brain Dynamics, National Institute of Mental Health, Porter Neuroscience Research Center, Bldg.…”

Section: Introductionmentioning

confidence: 99%

“…These cultures transition through different activity levels during postnatal maturation (Maeda, Robinson et al, 1995;Kamioka, Maeda et al, 1996;Corner, van Pelt et al, 2002;Johnson and Buonomano, 2007) and are well suited to identify a common network state that encompasses the variable features of the overall activity. We show that a power law with an exponent of α = −1.5, i.e.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Stewart

Plenz

2008

Journal of Neuroscience Methods

View full text Add to dashboard Cite

Cortical networks in vivo and in vitro are spontaneously active in the absence of inputs, generating highly variable bursts of neuronal activity separated by up to seconds of quiescence. Previous measurements in adult rat cortex revealed an intriguing underlying organization of these dynamics, termed neuronal avalanches, which is indicative of a critical network state. Here we demonstrate that neuronal avalanches persist throughout development in cortical slice cultures from newborn rats. More specifically, we find that in spite of large variations of average rate in activity, spontaneous bursts occur with power-law distributed sizes (exponent -1.5) and a critical branching parameter close to 1. Our findings suggest that cortical networks homeostatically regulate a critical state during postnatal maturation.

show abstract

Section: The Organization Of Spontaneous Synchronized Activity In Orgsupporting

confidence: 91%

Section: The Organization Of Spontaneous Synchronized Activity In Orgmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Stewart

Plenz

2008

Journal of Neuroscience Methods

View full text Add to dashboard Cite

show abstract

“…This effect is commonly observed and is very similar to the cell patterns seen in other patterned co-culture studies. [27,47] Studies show that random, dissociated neurons in culture develop physiological responses to neurotransmitters [48,49] and self-organize into neuronal networks, [49][50][51][52] however, they lack the structure normally present within the nervous system, where the neurons reside in specific regions with numerous network connectivity. The advantage of patterned co-cultures of neurons and astrocytes is that it can allow for precise control of both the direction of neurite extension and degree of contact between the neurons and astrocytes, unlike the random co-culture, which is important for repair and regeneration of the nervous systems.…”

Section: Patterned Versus Random Co-culturementioning

confidence: 99%

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Kidambi

Lee

Chan

2008

Adv Funct Materials

View full text Add to dashboard Cite

We engineered patterned co-cultures of primary neurons and astrocytes on polyelectrolyte multilayer (PEM) films without the aid of adhesive proteins/ligands to study the oxidative stress mediated by astrocytes on neuronal cells. A number of studies have explored engineering coculture of neurons and astrocytes predominantly using cell lines rather than primary cells owing to the difficulties involved in attaching primary cells onto synthetic surfaces. To our knowledge this is the first demonstration of patterned co-culture of primary neurons and astrocytes for studying neuronal metabolism. In our study, we used synthetic polymers, namely poly(diallyldimethylammoniumchloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, to build the multilayers. Primary neurons attached and spread preferentially on SPS surfaces, while primary astrocytes attached to both SPS and PDAC surfaces. SPS patterns were formed on PEM surfaces, either by microcontact printing SPS onto PDAC surfaces or vice-versa, to obtain patterns of primary neurons and patterned co-cultures of primary neurons and astrocytes. We further used the patterned co-culture system to study the neuronal response to elevated levels of free fatty acids as compared to the response in separated monoculture by measuring the level of reactive oxygen species (ROS; a widely accepted marker of oxidative stress). The elevation in the ROS levels was observed to occur earlier in the patterned co-culture system than in the separated monoculture system. The results suggest that this technique may provide a useful tool for engineering neuronal co-culture systems, that may more accurately capture neuronal function and metabolism, and thus could be used to obtain valuable

show abstract

Modification of glassy carbon surfaces with synthetic laminin-derived peptides for nerve cell attachment and neurite growth

Huber

Heiduschka

Kienle

et al. 1998

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Interactions between cultured nerve cells and surfaces are of importance for the implantation of biocompatible electrode materials such as glassy carbon (GC). Since implants serve as recording sensors in prosthetic neuroscience, we investigated whether coating electrodes with certain laminin derivatives containing the peptide sequences SIKVAV, CDPGYIGSR, PDSGR, YFQRYLI, and RNIAEIIKDA influences neuronal adhesion and neurite outgrowth in vitro. The coating of GC was performed by electrochemical polymerization and, for comparison, by adsorption or covalent coupling. Electrochemical polymerization is suitable for the coupling of peptides to GC, as shown by amino acid analysis and sequencing. Embryonic chicken retinal ganglion cells and brain cells (days E7 or E17) were used for both attachment and growth studies. Surfaces made by electrochemical polymerization of peptides were more efficient than those made by adsorption or covalent coupling of peptides. Synthetic cyclic peptide derivatives of CDPGYIGSR and 18-mer SIKVAV were found to be more efficient than the linear peptides. Competitive effects that resulted in a decreased cell attachment could be found upon application of soluble peptides. Nevertheless, irrespective of the method of coating, peptides were less efficient compared with the whole laminin molecule, as expected from its multiple adhesion sites. When small GC pins were implanted into the brain of E17 chicken after coating with the 18-mer SIKVAV peptide, nerve cell attachment was observed in vivo. The results suggest that chronically implantable materials may exert a higher neurocompatibility when coated with synthetic peptides.

show abstract

Spontaneous periodic synchronized bursting during formation of mature patterns of connections in cortical cultures

Cited by 332 publications

References 19 publications

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Modification of glassy carbon surfaces with synthetic laminin-derived peptides for nerve cell attachment and neurite growth

Contact Info

Product

Resources

About