Spontaneous rate of sister chromatid exchanges (SCEs) and BrdU dose-response relationships in mitotic chromosomes of goat (Capra hircus L.)

Abstract

The spontaneous level of sister chromatid exchange (SCEs) in the goat, estimated by exposing peripheral blood lymphocytes to 0.1 microgram/ml of 5-bromodeoxyuridine (BrdU), was 3.28 +/- 1.71 SCE/cell, 1.64 SCE/cell generation and 0.027 SCE/chromosome. The dose-response curve of SCE/cell, observed by exposing the cells to 0.1, 0.25, 0.5, 1.0, 2.5, and 5.0 micrograms/ml of BrdU, rose rapidly from 0.1 to 0.5 microgram/ml, remained fairly stable from 0.5 to 1.0 microgram/ml and rose less rapidly from 1.0 to 5.0 micrograms/ml of BrdU. The frequency distribution of sister chromatid exchanges/cell and that of chromosomes showing various number of exchanges followed the Poisson probability at all BrdU levels; only at 5.0 micrograms/ml of BrdU was the fit found on the border of the 5% probability level. The usefulness of determining the spontaneous level of SCE/cell in domestic animals is discussed in relation to its possible application for a more precise evaluation of the genotoxic effects of environmental pollutants.

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

“…Despite high numbers of identified SCEs per cell, a gradual decrease in the number of SCEs was observed with higher BrdU doses in comparison with the differences in SCE numbers identified between the second and the third concentrations. Similar correlation was also observed by Di Berardino et al (1995, 1996.…”

“…(1 was assumed to be optimal for spontaneous SCEs by Vijh et al (1992), Di Berardino et al (1995, 1996 and Arias (2000). Other researchers claimed 10 mg BrdU mL…”

“…(1 , were applied by Di Berardino et al (1995Berardino et al ( , 1996. They indicated that concentrations below 0.1 and 0.25 mg BrdU mL…”

The Effect of Environmental Factors on Sister Chromatid Exchange Incidence in Domestic Horse (Equus caballus) Chromosomes

“…WILSON & THOMPSON (2007) claim that the BrdU level should be very low or close to zero. The optimal BrdU dose is 5-30 Fg/ml (LEIN- BENGUTH & THIEL 1986;DI BERARDINO et al 1996;PERETTI et al 2006). In order to induce SCE, a 10 Fg/ml BrdU dose was used in the present study.…”

Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

“…According to Wilson & Thompson (2007), spontaneous SCEs are those that take place with a very low or zero level of BrdU. Leinbenguth & Thiel (1986) considered the concentration of 15-30 µg/ml to be the optimal dose of BrdU, Vijh et al (1992), Di Berardino et al (1995, 1996 and Arias (2000): 5µg/ml, whereas Ciotola et al (2005) and Peretti et al (2006Peretti et al ( , 2008: 10 µg/ml. Based on the results of the above authors, in experiments we applied the 10 µg/ml dose of BrdU in order to obtain spontaneous SCEs.…”