Sporotrichoid Cutaneous Infection With Mycobacterium kansasii

Owens, Donald W.

doi:10.1001/archderm.1969.01610250060014

Cited by 52 publications

(15 citation statements)

References 13 publications

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“…A sporotrichoid infection is a common cutaneous infectious disease that can be caused by various pathogens, including bacteria, fungi, leishmania, and atypical mycobacteria (12,13,14,22). Among the causative pathogens, atypical mycobacteria, especially M. marinum, and S. schenckii are the leading causes of a sporotrichoid lesion.…”

Section: Discussionmentioning

confidence: 99%

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Detection of Sporothrix schenckii in Clinical Samples by a Nested PCR Assay

Hu¹,

Chung²,

Hung

et al. 2003

J Clin Microbiol

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Cutaneous sporotrichosis is a chronic granulomatous fungal infection caused by Sporothrix schenckii with worldwide distribution. Its traditional diagnosis is time-consuming and difficult to differentiate from that of a clinical sporotrichoid lesion caused by various pathogens. In this study, a nested PCR assay for the detection of S. schenckii was evaluated by using a sequence of 18S rRNA gene as a target. For the examination of specificity and sensitivity, five clinical isolates with 1 ATCC 10213 strain of S. schenckii, 10 strains of clinical common fungi, 3 strains of Mycobacterium spp., Staphylococcus aureus, and normal human skin tissue were used. The expected fragment was amplified from six S. schenckii isolates in the first round and nested PCR but not from other microorganisms and human DNA. Their sequences were 100% identical to the S. schenckii 18S rRNA gene sequence deposited in GenBank. A detection limit of 40 fg of S. schenckii DNA extract was determined with ethidium bromide staining. Serial dilution studies demonstrated that the nested PCR could detect a DNA amount of 1 CFU of S. schenckii in tissue samples. We further investigated the nested PCR assay for the detection of S. schenckii from the tail tissues of 5 experimentally infected mice and from the clinical biopsy specimens of 12 patients with sporotrichosis confirmed by culture or histochemical staining. The nested PCR assay was positive in all 5 infected mice and in 11 of the 12 clinical specimens. The high sensitivity and specificity of this nested PCR indicate that the assay can provide rapid diagnosis with sufficient accuracy to be clinically useful for patients with sporotrichosis.

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Section: Discussionmentioning

confidence: 99%

“…A S. schenckii PCR assay may provide a more effective and rapid way to diagnosis sporotrichosis from clinical sporotrichoid infections that can be also caused by various other pathogens, including bacteria, fungi, leishmania, and atypical mycobacteria (12,13,14,22). Hence, this may significantly minimize health risks.…”

mentioning

confidence: 99%

Detection of Sporothrix schenckii in Clinical Samples by a Nested PCR Assay

Hu¹,

Chung²,

Hung

et al. 2003

J Clin Microbiol

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“…A few cases have been recorded in which cellulitis or sporotrichoid cutaneous infection was caused by M. kansasii (Maberry, Mullins & Stone, 1965;Owens & McBride, 1969;Fraser et al 1975;Hirsch & Saffold, 1976;Dore, Collins & Mankiewicz, 1979;Rosen, 1983). Similarly, infections, including sporotrichoid forms, have been ascribed to M. chelonei (Bernstadt, 1974;Greer, Grose & Martensen, 1979;Fenske & Millns, 1981), to M. szulqai (Sybert, Tsou & Garagusi, 1977), to unidentified slow-growing scotochromogens (Knox et al 1961;Cott, Carter & Sall, 1967), and to M. gordonae (Shelley & Folkens, 1984).…”

Section: Cutaneous Infections Caused By Other Mycobacteriamentioning

confidence: 99%

Mycobacterium marinuminfections in man

Collins

Grange

Noble

et al. 1985

J. Hyg.

101

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Mycobacterium marinum, formerly known as ‘Mycobacterium balnei’, is a free-living organism which causes disease in fish and occasionally in man. When such infections occur in man they are usually associated with some aquatic activity, e.g. swimming, fishing, boating and keeping tropical fish. For this reason, the organism has been called a ‘leisure-time pathogen’ by Feldman, Long & David (1974) and the disease a ‘hobby hazard’ by Heineman, Spitzer & Pianphongsant (1972).

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“…65 Skin lesions are rare but can be inoculated. 67 Histologically, the lesions show acute inflammation associated with some granulomatous response and numerous acid-fast bacilli. 67 Histologically, the lesions show acute inflammation associated with some granulomatous response and numerous acid-fast bacilli.…”

Section: Mycobacterium Kansasiimentioning

confidence: 99%