Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells

Kim, Jisun; Jeon, Jeha; Song, Bin; Lee, Nayeon; Ko, Sanghyeok; Cha, Young; Leblanc, Pierre; Seo, Hyemyung; Kim, Kwang-Soo

doi:10.1038/s41596-021-00673-4

Cited by 12 publications

(8 citation statements)

References 48 publications

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“…As a result of the overcrowding, some NSPCs separated from the hydrogels and lead to lower cell viability at day 5. [ 33 ]…”

Section: Resultsmentioning

confidence: 99%

“…As a result of the overcrowding, some NSPCs separated from the hydrogels and lead to lower cell viability at day 5. [33] The effect of the controlled release of PTX from DDS on cell migration and differentiation is still unclear. Here, we explored the effect of PTX release on NSPCs in vitro.…”

Section: Functional Hydrogels Promote the Neuronal Differentiation An...mentioning

confidence: 99%

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Individually Tailored Modular “Egg” Hydrogels Capable of Spatiotemporally Controlled Drug Release for Spinal Cord Injury Repair

Sun

Xiong

Yang

et al. 2023

Adv Healthcare Materials

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Controllable drug delivery systems (DDS) can overcome the disadvantages of conventional drug administration processes, such as high dosages or repeated administration. Herein, a smart DDS collagen hydrogel is deployed for spinal cord injury (SCI) repair based on modular designing of “egg” nanoparticles (NPs) that ingeniously accomplish controlled drug release via inducing a signaling cascade in response to external and internal stimuli. The “egg” NPs consist of a three‐layered structure: tannic acid/Fe3+/tetradecanol “eggshell,” zeolitic imidazolate framework‐8 (ZIF‐8) “egg white,” and paclitaxel “yolk.” Then NPs served as a crosslinking epicenter, blending with collagen solutions to generate functional hydrogels. Remarkably, the “eggshell” efficiently converts near‐infrared (NIR) irradiation into heat. Subsequently, tetradecanol can be triggered to disintegrate via heat, exposing the structure of ZIF‐8. The Zn‐imidazolium ion coordination bond of the “egg white” is susceptible to cleaving at the acidic SCI site, decomposing the skeleton to release paclitaxel on demand. As expected, the paclitaxel release rate upon NIR irradiation increased up to threefold on the seventh day, which matches endogenous neural stem/progenitor cell migration process. Taken together, the collagen hydrogels facilitate the neurogenesis and motor function recovery, demonstrating a revolutionary strategy for spatiotemporally controlled drug release and providing guidelines for the design of DDS.

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“…As a result of the overcrowding, some NSPCs separated from the hydrogels and lead to lower cell viability at day 5. [ 33 ]…”

Section: Resultsmentioning

confidence: 99%

Section: Functional Hydrogels Promote the Neuronal Differentiation An...mentioning

confidence: 99%

Individually Tailored Modular “Egg” Hydrogels Capable of Spatiotemporally Controlled Drug Release for Spinal Cord Injury Repair

Sun

Xiong

Yang

et al. 2023

Adv Healthcare Materials

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show abstract

“…Human stem cell-based in vitro assays can be of great use to study the potential effects of compounds on (developing) dopaminergic neurons. There is great progression in the differentiation of human stem cells towards a dopaminergic fate for therapeutical purposes (e.g., [ 20 , 21 ]). However, cell cultures, especially co-cultures, suited for the assessment of chemical exposure specifically to dopaminergic neurons are not yet well-developed, but urgently needed [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Prolonged Differentiation of Neuron-Astrocyte Co-Cultures Results in Emergence of Dopaminergic Neurons

Leeuw

Oostrom

Zwart

et al. 2023

IJMS

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Dopamine is present in a subgroup of neurons that are vital for normal brain functioning. Disruption of the dopaminergic system, e.g., by chemical compounds, contributes to the development of Parkinson’s disease and potentially some neurodevelopmental disorders. Current test guidelines for chemical safety assessment do not include specific endpoints for dopamine disruption. Therefore, there is a need for the human-relevant assessment of (developmental) neurotoxicity related to dopamine disruption. The aim of this study was to determine the biological domain related to dopaminergic neurons of a human stem cell-based in vitro test, the human neural progenitor test (hNPT). Neural progenitor cells were differentiated in a neuron-astrocyte co-culture for 70 days, and dopamine-related gene and protein expression was investigated. Expression of genes specific for dopaminergic differentiation and functioning, such as LMX1B, NURR1, TH, SLC6A3, and KCNJ6, were increasing by day 14. From day 42, a network of neurons expressing the catecholamine marker TH and the dopaminergic markers VMAT2 and DAT was present. These results confirm stable gene and protein expression of dopaminergic markers in hNPT. Further characterization and chemical testing are needed to investigate if the model might be relevant in a testing strategy to test the neurotoxicity of the dopaminergic system.

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“…This approach not only serves as a model for human brain development/neurological disease model to provide a new platform for researching the pathogenesis of related diseases and screening potential drugs but also brings new hope for the development of cell replacement therapy . However, the traditional process of differentiating hPSCs into neurons is intricate and time-consuming, and there is a lack of accurate quality control approach in the differentiation process, constraining its applicability in the investigation of neurological disease mechanisms and cell replacement therapy. − Therefore, there is a pressing need to enhance differentiation protocols, aiming to generate enriched neuronal populations with mature characteristics while simplifying the cumbersome culture conditions.…”

Section: Introductionmentioning

confidence: 99%

Integration of Magnetic-Field-Directed Self-Assembly-Based Cell Culture and Biosensing Platform for Improving hPSCs-Derived Neurons and Quantitative Detection of Neurotransmitter

Zhang,

Cao,

et al. 2023

ACS Appl. Mater. Interfaces

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Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells

Cited by 12 publications

References 48 publications

Individually Tailored Modular “Egg” Hydrogels Capable of Spatiotemporally Controlled Drug Release for Spinal Cord Injury Repair

Individually Tailored Modular “Egg” Hydrogels Capable of Spatiotemporally Controlled Drug Release for Spinal Cord Injury Repair

Prolonged Differentiation of Neuron-Astrocyte Co-Cultures Results in Emergence of Dopaminergic Neurons

Integration of Magnetic-Field-Directed Self-Assembly-Based Cell Culture and Biosensing Platform for Improving hPSCs-Derived Neurons and Quantitative Detection of Neurotransmitter

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