Spoxazomicins A–C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460T

Inahashi, Yuki; Iwatsuki, Masato; Ishiyama, Aki; Namatame, Miyuki; Nishihara‐Tsukashima, Aki; Matsumoto, Atsuko; Hirose, Tomoyasu; Sunazuka, Toshiaki; Yamada, Hideaki; Otoguro, Kazuhiko; Takahashi, Yōko; Ōmura, Satoshi; Shiomi, Kazuro

doi:10.1038/ja.2011.16

Cited by 89 publications

(57 citation statements)

References 9 publications

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“…As a result, we found the actinoallolides which are 12-membered macrolides in a cultured broth of Actinoallomurus fulvus MK10-036, the trehangelins which consist of two trehalose molecules and an angelic acid from Polymorphospora rubra K07-0510, and the spoxazomicins, pyochelin family antibiotics from Streptosporangium oxazolinicum K07-0460 T . [39][40][41] Furthermore, we have cultured 22 endophytic actinomycetes, which were isolated from eight kinds of plant and classified in 12 genera. The HPLC analyses of their culture broths extracted by ethyl acetate indicated that aranciamycins were produced in seven strains, belonging to the genera Sphaerisporangium, Kibdelosporangium, Planomonospora and Actinophytocola, and their cultured broths displayed antibacterial activity against Xanthomonas campestris pv.…”

Section: Search For Novel Compound From Endophytic Actinomycetesmentioning

confidence: 99%

Endophytic actinomycetes: promising source of novel bioactive compounds

2017

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Endophytic actinomycetes associated with plant roots are a relatively untapped source of potential new bioactive compounds. This is becoming increasingly important, as the returns from discovery research on soil-dwelling microbes, have been continuously diminishing. We have isolated more than 1000 strains of actinomycetes from plant roots in our search for novel bioactive compounds, identified and assayed their bioactive metabolites, as well as investigated their biosynthetic genes for generating secondary metabolites. This has resulted in the discovery of several interesting compounds. Creation of plant root clone libraries enabled us to confirm that we had, indeed, isolated endophytes. In this paper, we introduce our approach to this promising line of research, incorporating data from other publications, and illustrate the potential that endophytic actinomycetes offer as a new source of novel lead compounds.

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Section: Search For Novel Compound From Endophytic Actinomycetesmentioning

confidence: 99%

Endophytic actinomycetes: promising source of novel bioactive compounds

2017

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“…This research will be reported in the accompanying paper. 7 The strain K07-0460 T was phylogenetically identified as being a member of the genus Streptosporangium described by Couch. 8 At present, the genus Streptosporangium comprises 15 species and 2 subspecies: Streptosporangium roseum, 8 S. amethystogenes subsp.…”

Section: Introductionmentioning

confidence: 98%

Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins

et al. 2011

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An actinomycete strain K07-0460 T producing new antitrypanosomal antibiotics, spoxazomicins, was isolated from the roots of a variety of orchid collected in the subtropical Okinawa prefecture. The 16S ribosomal RNA gene sequence analysis indicated that the strain belonged to the genus Streptosporangium and showed high similarities with S. amethystogenes subsp. amethystogenes DSM 43179 T (99.4%), S. amethystogenes subsp. fukuiense IFO 15365 T (99.2%) and S. longisporum DSM 43180 T (98.7%). The DNA-DNA hybridization relatedness values between strain K07-0460 T and the three strains were below 70%. On the basis of phylogenetic analysis, DNA-DNA hybridization relatedness and physiological characteristics, the strain should be classified as a new species Streptosporangium oxazolinicum sp. nov. in the genus Streptosporangium. The type strain of S. oxazolinicum is K07-0460 T (¼JCM 17388 T ).

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“…[2][3][4][5] This time we focused on metabolites from cultures of cordyceps that showed antitrypanosomal activity. The "dong chóng xìa căo" in Chinese pronunciation, means "summer herb-winter worm" is traditionally used for herbal medicines and health foods in East to Southeast Asia.…”

Section: In Vitromentioning

confidence: 99%