SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement

Walter, Johanna‐Gabriela; Eilers, Alina; Alwis, Lourdes S. M.; Roth, Bernhard; Bremer, Kort

doi:10.3390/s20102889

Cited by 57 publications

(31 citation statements)

References 48 publications

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“…The fabricated sensor had better sensitivity and detection limit (5 pg/mL) in comparison to gold based system ( Xu et al, 2020 ). Recently waveguide based sensor system for CRP are reported where aptamer and antibody are used as recognition elements ( Walter et al, 2020 ). The system with aptamers as the recognition element had a wider linear range but the system with antibodies as the recognition element displayed a better detection limit ( Ashiba et al, 2020 ).…”

Section: Inflammatory Biomarkers and Their Detection Platformsmentioning

confidence: 99%

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Garg

Sharma

Singh

2021

Biosensors and Bioelectronics

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COVID-19 pandemic has affected everyone throughout the world and has resulted in the loss of lives of many souls. Due to the restless efforts of the researchers working hard day and night, some success has been gained for the detection of virus. As on date, the traditional polymerized chain reactions (PCR), lateral flow devices (LFID) and enzyme linked immunosorbent assays (ELISA) are being adapted for the detection of this deadly virus. However, a more exciting avenue is the detection of certain biomarkers associated with this viral infection which can be done by simply re-purposing our existing infrastructure. SARS-CoV-2 viral infection triggers various inflammatory, biochemical and hematological biomarkers. Because of the infection route that the virus follows, it causes significant inflammatory response. As a result, various inflammatory markers have been reported to be closely associated with this infection such as C-reactive proteins, interleukin-6, procalcitonin and ferritin. Sensing of these biomarkers can simultaneously help in understanding the illness level of the affected patient. Also, by monitoring these biomarkers, we can predict the viral infections in those patients who have low SARS-CoV-2 RNA and hence are missed by traditional tests. This can give more targets to the researchers and scientists, working in the area of drug development and provide better prognosis. In this review, we propose to highlight the conventional as well as the non-conventional methods for the detection of these inflammatory biomarkers which can act as a single platform of knowledge for the researchers and scientists working for the treatment of COVID-19.

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Section: Inflammatory Biomarkers and Their Detection Platformsmentioning

confidence: 99%

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Garg

Sharma

Singh

2021

Biosensors and Bioelectronics

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“…Upon target addition, the aptamer-target complex is formed and the wavelength shift is monitored. This smartphone-assisted sensor was successfully used for the detection of the C-reactive protein with a LOD of 12.46 nM (normal C-reactive protein (CRP) concentration in blood is 40 nM) [ 95 ]. The working principle of the sensor is shown in Figure 4 .…”

Section: Aptamers In Point-of-care Testing (Poct)mentioning

confidence: 99%

Aptasensors for Point-of-Care Detection of Small Molecules

et al. 2020

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Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.

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“…Through this process, the binding of the small molecule 25OHD, which is hard to detect by SPR in general, is translated into the dissociation of the larger oligonucleotide [18]. To further increase the 25OHD-induced changes of the refractive index above the sensor surface, gold nanoparticles (AuNPs) were used for signal enhancement, as described recently for aptamer-based detection of CRP [17].…”

Section: Sensor Designmentioning

confidence: 99%

“…After the splitter, the waveguide width of each channel was 100 µm and each waveguide was further tapered after the splitter element to obtain the final waveguide width of 50 µm. This particular waveguide width was chosen so that the resulting SPR sensor is comparable to our previous investigation of planar-optical SPR sensors [17]. Furthermore, a waveguide bend with an average radius of 5 mm was applied to align and guide the light from the flashlight LED to the camera of the smartphone.…”

Section: Fabrication Of Planar-optical Biosensor Chipmentioning

confidence: 99%

“…Previously, the authors have demonstrated the use of a simple white light LED and a spectrophotometer for the interrogation of an SPR-based sensor for biomarker detection [17]. The design proposed in this study aims to progress a step further through the utilization of a smartphone to provide the appropriate interrogation photonics in tandem with a microfluidic chip equipped with a two-channel SPR sensing scheme for biomarker detection.…”

Section: Introductionmentioning

confidence: 99%

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All-Optical Planar Polymer Waveguide-Based Biosensor Chip Designed for Smartphone-Assisted Detection of Vitamin D

Walter

Alwis

Roth

et al. 2020

Sensors

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An all-optical plasmonic sensor platform designed for smartphones based on planar-optical waveguide structures integrated in a polymer chip is reported for the first time. To demonstrate the applicability of the sensor system for biosensing purposes, the detection of 25-hydroxyvitamin D (25OHD) in human serum samples using an AuNP-enhanced aptamer-based assay was demonstrated. With the aid of the developed assay sensitivity of 0.752 pixel/nM was achieved for 25OHD concentrations ranging from 0–100 nM. The waveguide structure of the sensor enables miniaturisation and parallelisation, thus, demonstrates the potential for simultaneous detection of various analytes including biomarkers. The entire optical arrangement can be integrated into a single polymer chip which allows for large scale and cost-efficient sensor fabrication. The broad utilization and access of smartphone electronics make the proposed design most attractive for its wider use in lab-on-chip applications.

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SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement

Cited by 57 publications

References 48 publications

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Aptasensors for Point-of-Care Detection of Small Molecules

All-Optical Planar Polymer Waveguide-Based Biosensor Chip Designed for Smartphone-Assisted Detection of Vitamin D

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