Spray jet assembly interface for the coupling of reversed-phase narrow-bore liquid chromatography and Fourier transform infrared spectrometry

Use of CuO Particles as an Interface in LC-FTIR Analysis

“…Then, the mobile phase is allowed to completely evaporate so that the FTIR spectra of the pure separated substances deposited on the interface can be recorded. [16][17][18][19][20][21][22][23][24][25] Off-line hyphenation exhibits an advantage in that the interference from the mobile phase is completely eliminated, which in turn, improves the signal-to-noise ratio of the resultant FTIR spectra. Gagel et al have utilized the highly IR reflective surface of metals, such as aluminum, as the interface for collecting separated substances from the eluate, followed by recording the corresponding FTIR spectra in the reflection mode.…”

“…However, this approach is limited by issues of band distortion. [22][23][24] A plausible approach is to use a hot germanium plate as the interface. During the experiment, the eluate obtained after LC separation, which elute at different retention times, are continuously deposited on the hot germanium plate for completely evaporating the mobile phase.…”

Electrofilament deposition and off-column detection of analytes separated by capillary electrophoresis

“…In addition to traditional techniques such as absorbance and fluorescence, detection on planar substrates has been performed using a variety of information-rich techniques [1]. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) [2], Fourier transform infrared spectroscopy [3], and surface-enhanced Raman spectroscopy (SERS) [4] can provide specific structural information. Additionally, such techniques as post-column radionuclide detection [5], chemiluminescence [6], and fluorescence line-narrowing [7] have been used to detect analytes on planar substrates.…”

“…The microfluidic transfer methods that have been employed to date include direct deposition of CE effluent onto a conductive substrate [9] and blotting [10], spray-jet [3], and electrospray (ES) deposition onto substrates [11,12]. Previously, McLeod et al [12] used conventional ES for deposition of analyte solutions.…”

“…Subsequently, charge accumulates at the liquid surface and is drawn toward the counter electrode such that a liquid cone forms (Taylor cone). At a sufficiently high field (~2 kV for CH 3 OH solution), a liquid filament is emitted from the Taylor cone. At higher fields (approximately 3±5 kV), the liquid filament shortens and at a point that is 0±2 mm from the Taylor cone, the Rayleigh limit is reached wherein the droplet charge overcomes surface tension and the liquid filament rapidly expands (Rayleigh explosion).…”