Spread of potato virus YNTN in potato cultivars (Solanum tuberosum L.) with different levels of sensitivity

Mehle, Nataša; Kovač, Maja; Petrovič, Nataša; Pompe-Novak, Maruša; Baebler, Špela; Stres, Hana Krečič; Gruden, Kristina; Ravnikar, Maja

doi:10.1016/j.pmpp.2004.10.005

Cited by 27 publications

(25 citation statements)

References 32 publications

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“…The presence of PepMV in tomato roots and its release from the roots into water have already been shown for a PepMV EU isolate (12,34). The presence of PVY NTN and PSTVd in potato roots has also been shown previously (35,36), although the release of PVY and PSTVd from roots into water has not been shown previously, and thus our data here constitute the first evidence of this. In our study, PepMV-Ch2, PVY NTN , and PSTVd were detected in the nutrient solution using RT-qPCR from the first month after initiation of irrigation.…”

Section: Discussionsupporting

confidence: 84%

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Mehle

Gutiérrez-Aguirre

Prezelj

et al. 2014

Appl Environ Microbiol

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Hydroponic systems and intensive irrigation are used widely in horticulture and thus have the potential for rapid spread of water-transmissible plant pathogens. Numerous plant viruses have been reported to occur in aqueous environments, although information on their survival and transmission is minimal, due mainly to the lack of effective detection methods and to the complexity of the required transmission experiments. We have assessed the role of water as a source of plant infection using three mechanically transmissible plant pathogens that constitute a serious threat to tomato and potato production: pepino mosaic virus (PepMV), potato virus Y (PVY), and potato spindle tuber viroid (PSTVd). PepMV remains infectious in water at 20 ؎ 4°C for up to 3 weeks, PVY (NTN strain) for up to 1 week, and PSTVd for up to 7 weeks. Experiments using a hydroponic system show that PepMV (Ch2 genotype) and PVY (NTN strain) can be released from plant roots into the nutrient solution and can infect healthy plants through their roots, ultimately spreading to the green parts, where they can be detected after a few months. In addition, tubers developed on plants grown in substrate watered with PSTVd-infested water were confirmed to be the source of viroid infection. Our data indicate that although well-known pathways of virus spread are more rapid than water-mediated infection, like insect or mechanical transmission through leaves, water is a route that provides a significant bridge for rapid virus/ viroid spread. Consequently, water should be taken into account in future epidemiology and risk assessment studies.

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Section: Discussionsupporting

confidence: 84%

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Mehle

Gutiérrez-Aguirre

Prezelj

et al. 2014

Appl Environ Microbiol

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“…One reason for this phenomenon could be the fact that because of high symptom severity leaves degraded and the viruses moved to other parts of the plant like in senescent leaves (Kogovšek et al 2011). In a related study Hühnlein et al (2013) compared the virus concentration of a heavily infectious PVY N W isolate with milder O and N strains in potato tubers and did not find higher virus concentrations in tissue of the potato tuber infected with PVY N W. Also Mehle et al (2004) did not observe any correlation between severity of virus symptoms and virus amount evaluated by ELISA titres. Therefore, virus concentration cannot be regarded as a useful measure to classify virus symptom severity.…”

Section: Discussionmentioning

confidence: 99%

Potato virus Y (PVY) in Seed Potato Certification

et al. 2015

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In the EU and many other countries worldwide, seed potatoes require certification to be marketed. In most of the European national and international seed potato regulations, the tolerances for viruses are at present based on a combination of virus incidence and symptom severity. Historically all severe viruses were usually the origin of severe virus diseases, and mild viruses, in general, were seen to cause mild diseases. However, the situation has changed, especially for the most economically important virus, Potato virus Y (PVY), which is considered a severe virus. Investigation of PVY-infected potato leaf samples from Bavarian potato seed certification found that the "classical" O and the N strains, predominantly responsible for severe virus diseases, represented less than 1% of the infections present. Ca. 99% of the PVY infections were comprised of the new recombinant strains PVY NTN and PVY N W. The ratio between these recombinants differed substantially. Differences in susceptibility of particular cultivars to certain PVY strains seem to be one reason for that. Therefore, the ratio of the recombinants may depend upon which cultivars are favoured in different potato growing areas or countries.In greenhouse tests, PVY NTN and PVY N W were associated with both severe and mild disease symptoms. In corresponding field tests, the symptom severity differed considerably. Furthermore, there was no correlation detected between virus concentration and symptom severity.These results will contribute to a worldwide discussion on the elimination of the criterion virus symptom severity from potato seed certification during the forthcoming months. The discussion follows amendments made to international seed potato regulation documents. National regulations must be aligned by the EU member states until January 2016.

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“…PVY-SASA-61 was the only isolate that developed necrotic local lesions on Capsicum frutescens (Fig. 3) (Mehle et al 2004). PVY NTN -Slo caused tuber necrotic ringspot disease (PTNRD) in cultivar Igor (Kus 1994).…”

Section: Sequence Analysesmentioning

confidence: 99%

Comparison of Genome Sequence of PVY Isolates with Biological Properties

Barker¹,

McGeachy²,

Toplak

et al. 2009

Am. J. Pot Res

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The whole RNA genomes of six European Potato virus Y (PVY) isolates, representing isolates of the PVY O , PVY N and PVY NTN subgroups, were sequenced and the reactions of a range of tobacco and potato cultivars after the inoculation were determined. One isolate induced symptoms of veinal necrosis when inoculated to tobacco, but reacted with PVY O -specific antibodies. The genome sequence of this isolate showed that it had a recombinant genome comprising large sections of sequence that are characteristic of N and O sub-group isolates. Another isolate caused mosaic symptoms when inoculated to tobacco, and reacted with PVY N -specific antibodies. The nucleotide sequence of its genome was very closely related to the genome sequence published for two potato-infecting PVY isolates from North America. These three isolates are the members of interesting group NA (North American). They have highly similar genome sequences throughout their length, but biologically represent isolates from the PVY O , PVY N and PVY NTN subgroups. The occurrence of these biologically diverse isolates with their highly homologous genomes brings into question the exact nature of gene structure in relation to function.Resumen Se secuenciaron los genomas completos de ARN de seis aislamientos europeos del virus Y de la papa (PVY) representantes de aislamientos de los subgrupos de PVY O , PVY N y PVY NTN y se determinaron las reacciones de una serie de cultivares de tabaco y papa después de las inoculaciones. Un aislamiento indujo síntomas de necrosis de las nervaduras cuando fue inoculado en tabaco pero reaccionó con los anticuerpos específicos de PVY O . La secuencia del genoma de este aislamiento mostró que tenía un genoma recombinante con grandes secciones de secuencias que son características de aislamientos de los subgrupos N y O. Otro aislamiento causó síntomas de mosaico cuando se inoculó a tabaco y reaccionó con anticuerpos específicos de PVY N . La secuencia de nucleótidos de su genoma fue relacionada muy cercanamente con la secuencia publicada de dos aislamientos de PVY de Norteamérica que infectan papa. Estos tres aislamientos son miembros del grupo NA (Norte Americano). Ellos tienen una secuencia de genoma altamente similar, pero biológicamente representan aislamientos de los subgrupos PVY O , PVY N y PVY NTN . La presencia de estos aislamientos biológica-mente diferentes con sus genomas altamente homólogos, pone en cuestión la naturaleza exacta del gen con relación a su función.

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Spread of potato virus YNTN in potato cultivars (Solanum tuberosum L.) with different levels of sensitivity

Cited by 27 publications

References 32 publications

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

Potato virus Y (PVY) in Seed Potato Certification

Comparison of Genome Sequence of PVY Isolates with Biological Properties

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