Spreading and actin cytoskeleton organization of cartilage and bone marrow stromal cells cocultured on various extracellular matrix proteins

Sachenberg, E. I.; Nikolaenko, N. N.; Пинаев, Г. П.

doi:10.1134/s1990519x15010083

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…MSCs showed heterogeneity in size and morphology. Following [43,44], we distinguished the following morphological types (Figure 4b): (1) small-sized unflattened cells; (2) medium-sized disc-shaped or elongated cells with F-actin in subplasmalemmal spaces (as a ring or in a leading edge); (3) large-sized flattened cells with pseudopodia. The MSC population on collagen was enriched in small-sized MSCs, whereas the largesized flattened-cell MSCs were abundant on dcECM.…”

Section: Attachment and Spreading Of Mscs On Decellularized Matricesmentioning

confidence: 99%

“…These observations can be interpreted as follows. On the one hand, it has previously been well demonstrated that fibroblasts undergo the consecutive steps of cell shape changes during spreading and movement during the first 2-24 h after attachment onto plastic ware [43][44][45], namely: disc-shaped, polarized with active edge, elongated fibroblast-like. Thus, the different shapes of MSCs at the same time point may correspond to the intensity of spreading and the potential for movement.…”

Section: Attachment and Spreading Of Mscs On Decellularized Matricesmentioning

confidence: 99%

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Hypoxic Extracellular Matrix Preserves Its Competence after Expansion of Human MSCs under Physiological Hypoxia In Vitro

Matveeva,

Buravkov,

Andreeva

et al. 2023

Biomimetics

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Tissue-relevant O2 levels are considered as an important tool for the preconditioning of multipotent mesenchymal stromal cells (MSCs) for regenerative medicine needs. The present study investigated the quality and functions of the extracellular matrix (ECM) of MSCs under low O2 levels. Human adipose tissue-derived MSCs were continuously expanded under normoxia (20% O2, N) or “physiological” hypoxia (5% O2, Hyp). Decellularized ECM (dcECM) was prepared. The structure of the dcECM was analyzed using confocal laser and scanning electron microscopy. Collagen, dcECM-N, and dcECM-Hyp were recellularized with MSC-N and further cultured at normoxia. The efficacy of adhesion, spreading, growth, osteogenic potential, and paracrine activity of recellularized MSC-N were evaluated. At low O2, the dcECM showed an increased alignment of fibrillar structures and provided accelerated spreading of MSC-N, indicating increased dcECM-Hyp stiffness. We described O2-dependent “ECM-education” of MSC-N when cultured on dcECM-Hyp. This was manifested as attenuated spontaneous osteo-commitment, increased susceptibility to osteo-induction, and a shift in the paracrine profile. It has been suggested that the ECM after physiological hypoxia is able to ensure the maintenance of a low-commitment state of MSCs. DcECM, which preserves the competence of the natural microenvironment of cells and is capable of “educating” others, appears to be a prospective tool for guiding cell modifications for cell therapy and tissue engineering.

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Section: Attachment and Spreading Of Mscs On Decellularized Matricesmentioning

confidence: 99%

Section: Attachment and Spreading Of Mscs On Decellularized Matricesmentioning

confidence: 99%

Hypoxic Extracellular Matrix Preserves Its Competence after Expansion of Human MSCs under Physiological Hypoxia In Vitro

Matveeva,

Buravkov,

Andreeva

et al. 2023

Biomimetics

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Extracellular Matrix in the Regulation of Stem Cell Differentiation

Novoseletskaya

Grigorieva

Efimenko

et al. 2019

Biochemistry Moscow

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Mesenchymal stromal cells application for experimental genital tuberculosis combination therapy

Afanaseva

Niauri

Vinogradova

et al. 2022

Journal of obstetrics and women's diseases

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BACKGROUND: Cell therapy is a promising trend in treating characterized by inflammation and sclerosis diseases such as genital tuberculosis, marked by pelvic inflammatory disease and uterine tube occlusion. AIM: Efficacy evaluation of combined mesenchymal stem cells and specific polychemotherapy treatment of the experimental genital tuberculosis. MATERIALS AND METHODS: Chinchilla rabbits (n = 27) were divided on 4 groups. Group 1 (n = 6) comprised control group, healthy animals. Group 2 (n = 7) infected with genital tuberculosis, without treatment. Group 3 (n = 7) infected with genital tuberculosis, treated with anti-tuberculous drugs only. Group 4 (n = 7) infected with genital tuberculosis, treated with anti-tuberculous drugs and mesenchymal stem cells. Culture of M. tuberculosis Erdman in dose 107 CFU/0.2 ml was injected under the left uterine tube serosa for the genital tuberculosis modelling. For treatment efficacy evaluation following tests were used: full blood count, blood chemistry, hysterosalpingography, diagnostic laparoscopy, peritoneal macrophage phagocytic activity assessment. RESULTS: Infected with genital tuberculosis animals had leukocytosis, elevated C-reactive protein, swelled and occluded uterine tubes. Treated with anti-tuberculous drugs and mesenchymal stem cells rabbits had lower white blood cell count (8.18 1.39 109/L vs 9.32 1.36 109/L, р 0.05) and C-reactive protein (1.1 0.8 mg/L vs 2.2 1.2 mg/L, р 0.01) compared to animal treated with anti-tuberculous drugs only. Animals in group 4 had also increased peritoneal macrophage phagocytic activity. Treated with mesenchymal stem cells animals had unobstructed uterine tubes, stabilized adhesive process within small pelvis with reparative process prevalence. CONCLUSIONS: Mesenchymal stem cells combined with anti-tuberculous drugs therapy favors reparative process in uterine tubes in genital tuberculosis.

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Spreading and actin cytoskeleton organization of cartilage and bone marrow stromal cells cocultured on various extracellular matrix proteins

Cited by 5 publications

References 17 publications

Hypoxic Extracellular Matrix Preserves Its Competence after Expansion of Human MSCs under Physiological Hypoxia In Vitro

Hypoxic Extracellular Matrix Preserves Its Competence after Expansion of Human MSCs under Physiological Hypoxia In Vitro

Extracellular Matrix in the Regulation of Stem Cell Differentiation

Mesenchymal stromal cells application for experimental genital tuberculosis combination therapy

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