Sprint training increases human skeletal muscle Na(+)-K(+)-ATPase concentration and improves K+ regulation

Abstract

This study investigated the effects of sprint training on muscle Na(+)-K(+)-adenosinetriphosphatase (ATPase) concentration, plasma [K+] regulation, muscle performance, and fatigue during severe intermittent exercise. Six untrained male subjects underwent intensive cycle-sprint training for 7 wk. Muscle biopsies were taken at rest from the vastus lateralis muscle before and after 7 wk of training and were assayed for Na(+)-K(+)-ATPase concentration using vanadate-facilitated [3H]ouabain binding to intact samples. Before and after the training period, subjects performed four maximal 30-s exercise bouts (EB) on a cycle ergometer, each separated by a 4-min recovery. Arterialized venous blood samples were drawn immediately before and after each sprint bout and were analyzed for plasma [K+]. The work output was significantly elevated (11%) across all four EBs after training. The muscle [3H]ouabain binding site concentration was significantly increased (16%) from 333 +/- 19 to 387 +/- 15 (SE) pmol/g wet wt after training but was unchanged in muscle obtained from three control subjects. Plasma [K+] rose by 1-2 mmol/l with each EB and declined rapidly by the end of each recovery period. The increases in plasma [K+] resulting from each EB were significantly lower (19%) after training. The ratios of rise in plasma [K+] relative to work output during each EB were also significantly lower (27%) after training. The increased muscle [3H]ouabain binding site concentration and the reduced ratio of rise in [K+] relative to work output with exercise are both consistent with improved plasma and skeletal muscle K+ regulation after sprint training.

High-Intensity Training Improves Plasma Glucose and Acid-Base Regulation During Intermittent Maximal Exercise in Type 1 Diabetes

“…Subjects completed four 30-s maximal exercise bouts (EB1-4) (each separated by 4 min rest) on a cycle ergometer. Supervised high-intensity cycling training (5,15,16) was then conducted thrice-weekly for 7 weeks. The number of cycle bouts per training session progressed from 4 in week 1 to 6 in week 2, 8 in week 3, and 10 in weeks 4 -7.…”

Adrenergic-β2 receptor polymorphism and athletic performance

“…67 It is thus possible that training regimes can take advantage of the force production capacity of skeletal muscles through the implementation of high-intensity sprint training exercises to increase Na + K + -ATPase concentration and thus increase the amount of force produced by muscle fibers on stimulation. 68 The polymorphism of subjects expressing homozygous Gly16 alleles demonstrated greater potential for trainability because of higher aerobic performances and increased responses to exercises. 38 Snyder et al 20 also proposed that the Gly16Glu27 haplotype represents a more positive response to exercise.…”

Adaptations to exercise training within skeletal muscle in adults with type 2 diabetes or impaired glucose tolerance: a systematic review

“…One randomized controlled trial reported increases of Na, K-pump content [21], which has been demonstrated to be low in patients with type 2 diabetes compared to healthy adults [45,46] and has been reported to increase both with endurance and sprint training in healthy individuals [47][48][49][50][51][52]. The intracellularly produced lactate leaves the cell via simple diffusion and two proteins: monocarboxylate transporter (MCT) 1 and MCT4 [53].…”