Src family tyrosine kinases mediate contraction of rat isolated tail arteries in response to a hyposmotic stimulus

Wijetunge, S; Hughes, Alun D.

doi:10.1097/hjh.0b013e328255e8f0

Cited by 15 publications

(10 citation statements)

References 62 publications

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“…Swelling of VSM may also induce cell contraction through activation of LTCC. Reports from cerebral artery VSM, canine basilar artery VSM, and rat-tail artery VSM confirmed the involvement of LTCC α 1c in vasoconstriction to a hypo-osmotic challenge (Kimura et al, 2000; Welsh, Nelson, Eckman, & Brayden, 2000; Wijetunge & Hughes, 2007). …”

Section: Plasmalemmal Ca2+-permeable Channelsmentioning

confidence: 75%

Calcium Channels in Vascular Smooth Muscle

Ghosh

Syed

Prada

et al. 2017

Advances in Pharmacology

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Calcium (Ca2+) plays a central role in excitation, contraction, transcription, and proliferation of vascular smooth muscle cells (VSMs). Precise regulation of intracellular Ca2+concentration ([Ca2+]i) is crucial for proper physiological VSM function. Studies over the last several decades have revealed that VSMs express a variety of Ca2+-permeable channels that orchestrate a dynamic, yet finely tuned regulation of [Ca2+]i. In this review, we discuss the major Ca2+-permeable channels expressed in VSM and their contribution to vascular physiology and pathology.

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Section: Plasmalemmal Ca2+-permeable Channelsmentioning

confidence: 75%

Calcium Channels in Vascular Smooth Muscle

Ghosh

Syed

Prada

et al. 2017

Advances in Pharmacology

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“…Among the numerous src-related kinases, c-src has been reported to be highly expressed in VSMCs. It has been implicated in the regulation of vascular smooth muscle tone [21, 22], and has been reported to modulate L -type Ca 2+ channel activity [23, 24], and to regulate TRPC6 channel activity by direct phosphorylation [13]. …”

Section: Discussionmentioning

confidence: 99%

Effect of Organ Culture on Noradrenaline- Evoked Contraction, Calcium Signalling and TRPC Expression in Rat Mesenteric Artery

Tai¹,

Vandenberg²,

Hamaide

et al. 2009

J Vasc Res

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The aim of this study was to explore the changes evoked by organ culture in the signalling pathways activated by noradrenaline in rat resistance mesenteric artery. Contractile responses and calcium signalling were significantly more sensitive to noradrenaline in arteries cultured for 48–72 h in the absence of growth factors compared to fresh arteries. Both calcium release activated by noradrenaline in calcium-free solution and calcium entry measured after the addition of external calcium were higher in cultured arteries than in fresh tissue. Blockers of non-selective cation channels (SKF-96365, flufenamic acid, Gd³⁺) more potently inhibited noradrenaline contraction in cultured arteries than in fresh ones. The src kinase inhibitors genistein or PP2 normalised the increased contraction and the increased calcium release evoked by noradrenaline in cultured arteries. In cultured arteries, trpc1 (transient receptor potential canonical 1) mRNA expression was decreased by 47 ± 8% (n = 5, p < 0.05), while trpc6 mRNA expression was increased by 92 ± 24% (n = 5, p < 0.05) in comparison with non-cultured arteries. Immunofluorescence analysis of protein expression confirmed the up-regulation of TRPC6 protein after culture. These results indicate that mesenteric artery culture results in src kinase-dependent increase in the responses to noradrenaline and in a change in cation channel activity, which could contribute to the increased contraction.

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“…However, an increasing number and variety of other signalling events are being found to modulate this excitation–contraction coupling. One recently identified player is c‐src (Wijetunge and Hughes 1996; 2007; Ibitayo et al. , 1998; Ishida et al.…”

Section: Introductionmentioning

confidence: 99%

The NADPH oxidase inhibitor diphenyleneiodonium is also a potent inhibitor of cholinesterases and the internal Ca²⁺ pump

Tazzeo

Worek²,

Janssen

2009

British J Pharmacology

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Background and purpose: Diphenyleneiodonium (DPI) is often used as an NADPH oxidase inhibitor, but is increasingly being found to have unrelated side effects. We investigated its effects on smooth muscle contractions and the related mechanisms. Experimental approach: We studied isometric contractions in smooth muscle strips from bovine trachea. Cholinesterase activity was measured using a spectrophotometric assay; internal Ca 2+ pump activity was assessed by Ca 2+ uptake into smooth muscle microsomes. Key results: Contractions to acetylcholine were markedly enhanced by DPI (10 -4 M), whereas those to carbachol (CCh) were not, suggesting a possible inhibition of cholinesterase. DPI markedly suppressed contractions evoked by CCh, KCl and 5-HT, and also unmasked phasic activity in otherwise sustained responses. Direct biochemical assays confirmed that DPI was a potent inhibitor of acetylcholinesterase and butyrylcholinesterase (IC50~8 ¥ 10 -6 M and 6 ¥ 10 -7 M, respectively), following a readily reversible, mixed non-competitive type of inhibition. The inhibitory effects of DPI on CCh contractions were not mimicked by another NADPH oxidase inhibitor (apocynin), nor the Src inhibitors PP1 or PP2, ruling out an action through the NADPH oxidase signalling pathway. Several features of the DPI-mediated suppression of agonist-evoked responses (i.e. suppression of peak magnitudes and unmasking of phasic activity) are similar to those of cyclopiazonic acid, an inhibitor of the internal Ca 2+ pump. Direct measurement of microsomal Ca 2+ uptake revealed that DPI modestly inhibits the internal Ca 2+ pump. Conclusions and implications: DPI inhibits cholinesterase activity and the internal Ca 2+ pump in tracheal smooth muscle.

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Src family tyrosine kinases mediate contraction of rat isolated tail arteries in response to a hyposmotic stimulus

Cited by 15 publications

References 62 publications

Calcium Channels in Vascular Smooth Muscle

Calcium Channels in Vascular Smooth Muscle

Effect of Organ Culture on Noradrenaline- Evoked Contraction, Calcium Signalling and TRPC Expression in Rat Mesenteric Artery

The NADPH oxidase inhibitor diphenyleneiodonium is also a potent inhibitor of cholinesterases and the internal Ca²⁺ pump

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Src family tyrosine kinases mediate contraction of rat isolated tail arteries in response to a hyposmotic stimulus

Cited by 15 publications

References 62 publications

Calcium Channels in Vascular Smooth Muscle

Calcium Channels in Vascular Smooth Muscle

Effect of Organ Culture on Noradrenaline- Evoked Contraction, Calcium Signalling and TRPC Expression in Rat Mesenteric Artery

The NADPH oxidase inhibitor diphenyleneiodonium is also a potent inhibitor of cholinesterases and the internal Ca2+ pump

Contact Info

Product

Resources

About

The NADPH oxidase inhibitor diphenyleneiodonium is also a potent inhibitor of cholinesterases and the internal Ca²⁺ pump