SRSF3: Newly discovered functions and roles in human health and diseases

More, Dhanashree Anil; Kumar, Arun

doi:10.1016/j.ejcb.2020.151099

Cited by 33 publications

(26 citation statements)

References 102 publications

(140 reference statements)

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“…However, KIRC was an exception to this situation. The results of SRSF3 were reported systemically in human pan-cancer first by us and it may indicate that SRSF3 may act as an oncogenic driver and an interesting biomarker for tumor monitoring in further research ( 29 , 30 ).…”

Section: Discussionmentioning

confidence: 74%

SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer

Huang

et al. 2022

Front. Oncol.

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Serine-rich splicing factor3 (SRSF3) plays an essential role in cell proliferation and inducing and maintaining of cancers as a proto-oncogene. However, the mechanisms of SRSF3 in pan-cancers are still unknown. In our study, a visualized prognostic landscape of SRSF3 in pan-cancer was investigated and the relationship between SRSF3 expression and immune infiltration was also investigated. The expression pattern and prognostic worth of SRSF3 among pan-cancers were explored through different databases, namely, the TCGA and Kaplan–Meier Plotter. Moreover, the survival analysis including Kaplan-Meier method for evaluating between groups was conducted. Further analyses including the correlation between expression SRSF expression and immune infiltration including tumor mutation burden (TMB), microsatellite instability (MSI) was investigated using Spearman test. In ACC, KIRP and UCEC cancer, upregulated expression of SRSF3 was associated with worse disease-free interval (DFI), representing a mechanism in promoting progression of tumor. Our results showed that SRSF3 expression was positively correlated immune cell infiltration, TMB, MSI in certain cancer types, indicating SRSF3 expression to potential value of therapy response. Additionally, we explored the functional characteristics of SRSF in vitro through western blot detecting the expression level of the apoptosis-related proteins in SW480 and 786-O cells. SRSF3 expression was upregulated in pan-cancer tissue compared with normal tissue, which confirmed by immunohistochemistry and its expression indicated poor overall survival and death-specific survival. Therefore, SRSF3 was found to be a possible biomarker for prognostic and therapeutic assessment through bioinformatic analysis. SRSF3 is expressed in various cancers and its high expression correlated to poor survival and disease progression. In summary, SRSF3 expression can be considered as a prognostic biomarker in pan-cancer and therapeutic evaluation.

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Section: Discussionmentioning

confidence: 74%

SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer

Huang

et al. 2022

Front. Oncol.

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“…Furthermore, LOC781276 attracted our attention as a serine/arginine-rich splicing factor 12 (SRSF12) pseudogene. SRSF12 is involved in the regulation of mRNA selective splicing and spliceosome tri-snRNP complex assembly [ 60 , 61 ]. Many studies clarified that the selective RNA splicing of key gene has an irreplaceable contribution to pigmentation [ 62 ] and muscle development [ 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

Hereditary Basis of Coat Color and Excellent Feed Conversion Rate of Red Angus Cattle by Next-Generation Sequencing Data

Huang

Wang

et al. 2022

Animals

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Angus cattle have made remarkable contributions to the livestock industry worldwide as a commercial meat-type breed. Some evidence supported that Angus cattle with different coat colors have different feed-to-meat ratios, and the genetic basis of their coat color is inconclusive. Here, genome-wide association study was performed to investigate the genetic divergence of black and red Angus cattle with 63 public genome sequencing data. General linear model analysis was used to identify genomic regions with potential candidate variant/genes that contribute to coat color and feed conversion rate. Results showed that six single nucleotide polymorphisms (SNPs) and two insertion–deletions, which were annotated in five genes (ZCCHC14, ANKRD11, FANCA, MC1R, and LOC532875 [AFG3-like protein 1]), considerably diverged between black and red Angus cattle. The strongest associated loci, namely, missense mutation CHIR18_14705671 (c.296T > C) and frameshift mutation CHIR18_12999497 (c.310G>-), were located in MC1R. Three consecutive strongly associated SNPs were also identified and located in FANCA, which is widely involved in the Fanconi anemia pathway. Several SNPs of highly associated SNPs was notably enriched in ZCCHC14 and ANKRD11, which are related to myofiber growth and muscle development. This study provides a basis for the use of potential genetic markers to be used in future breeding programs to improve cattle selection in terms of coat color and meat phenotype. This study is also helpful to understand the hereditary basis of different coat colors and meat phenotypes. However, the putative candidate genes or markers identified in this study require further investigation to confirm their phenotypic causality and potential effective genetic relationships.

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“…SRSF3 is an SR family protein that possesses an N-terminal RNA-binding RNA recognition motif (RRM) and a C-terminal arginine/serine (RS)-rich domain responsible for protein-protein and protein-RNA interactions. SRSF3 is a multifunctional splicing factor involved in transcriptional, co-transcriptional, and post-transcriptional regulation and has been implicated in a variety of human pathologies including heart disease, Alzheimer’s, and cancer (More & Kumar, 2020; Zhou et al, 2020). Examination of our paired RNA-seq and Ribo-seq data revealed robust expression of SRSF3 NMD-targeted exon 4 after 8 h of DUX4 induction, followed by robust translation of this exon that ends at the site of the PTC ( Figure 5A ).…”

Section: Resultsmentioning

confidence: 99%

Truncated RNA-binding protein production by DUX4-induced systemic inhibition of nonsense-mediated RNA decay

Campbell

Dyle

Matheny

et al. 2021

Preprint

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DUX4 is an embryonic transcription factor whose misexpression in skeletal muscle causes facioscapulohumeral muscular dystrophy (FSHD). DUX4 dysregulates multiple pathways that could contribute to FSHD pathophysiology. However, lack of temporal data and the knowledge of which RNAs are actively translated following DUX4 expression has hindered our understanding of the cascade of events that lead to muscle cell death. Here, we interrogate the DUX4 transcriptome and translatome over time and find dysregulation of most key pathways as early as 4 hours after DUX4 induction, demonstrating the potent effect of DUX4 in disrupting muscle homeostasis. We also observe extensive transcript downregulation as well as induction, and a high concordance between mRNA abundance and translation status. Significantly, DUX4 triggers widespread production of truncated protein products derived from aberrant RNAs that are degraded in normal muscle cells. One such protein, truncated serine/arginine-rich splicing factor 3 (SRSF3-TR), is present in FSHD muscle cells and disrupts splicing autoregulation when ectopically expressed in myoblasts. Taken together, the temporal dynamics of DUX4 induction show how the pathologic presence of an embryonic transcription factor in muscle cells alters gene expression at all levels of the central dogma.

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SRSF3: Newly discovered functions and roles in human health and diseases

Cited by 33 publications

References 102 publications

SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer

SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer

Hereditary Basis of Coat Color and Excellent Feed Conversion Rate of Red Angus Cattle by Next-Generation Sequencing Data

Truncated RNA-binding protein production by DUX4-induced systemic inhibition of nonsense-mediated RNA decay

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