Burley tobacco is a light air-cured tobacco primarily used in the production of white and kretek cigarettes. Currently the major cultivated variety (TN90) has declined in its performance. To overcome the problem, several new high-quality and productivity genotypes have been introduced. However, these new genotypes have to be evaluated before they are cultivated commercially. This research aimed to determine the yield potential and adaptability of the newly introduced Burley tobacco genotypes for two growing seasons in six locations. Four introduced genotypes (AOB359, AOB656, DBH454, NC7LC) and one check variety (TN90) were arranged in a randomized block design with five replications. Parameters observed included flowering age, leaf number, leaf length and width, yield of dried tobacco leaves, grade index, and crop index. Data were analyzed using PKBT STAT 2.03. The results showed that yield potential varied from 1294.8 – 1527.3 kg ha−1 for dried tobacco leaves, grade index ranged from 55.25 – 55.91, crop index ranged from 70.91 - 84.60 and wide adaptability. The AOB359, AOB656, and NC7LC genotypes produced a potentially higher yield than the check variety.