Stability improvement of immobilized Candida antarctica lipase B in an organic medium under microwave radiation

Rejasse, Barbara; Lamare, Sylvain; Legoy, Marie‐Dominique; Besson, Thierry

doi:10.1039/b401145g

Cited by 69 publications

(39 citation statements)

References 12 publications

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“…[265] Apart from traditional organic and combinatorial synthesis protocols covered in this Review (see Section 2), more recent applications of microwave chemistry include biochemical processes such as a high-speed polymerase chain reaction (PCR), [266] rapid enzyme-mediated protein mapping, [267] and general enzyme-mediated organic transformations (biocatalysis). [268] Furthermore, microwaves have been used in conjunction with electrochemical [269] and photochemical processes, [270] and are also employed in polymer chemistry [271] and material science applications, [272] such as the fabrication and modification of carbon nanotubes or nanowires. [273] So why isn t everybody using microwaves?…”

Section: Methodsmentioning

confidence: 99%

Controlled Microwave Heating in Modern Organic Synthesis

2004

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Although fire is now rarely used in synthetic chemistry, it was not until Robert Bunsen invented the burner in 1855 that the energy from this heat source could be applied to a reaction vessel in a focused manner. The Bunsen burner was later superseded by the isomantle, oil bath, or hot plate as a source for applying heat to a chemical reaction. In the past few years, heating and driving chemical reactions by microwave energy has been an increasingly popular theme in the scientific community. This nonclassical heating technique is slowly moving from a laboratory curiosity to an established technique that is heavily used in both academia and industry. The efficiency of “microwave flash heating” in dramatically reducing reaction times (from days and hours to minutes and seconds) is just one of the many advantages. This Review highlights recent applications of controlled microwave heating in modern organic synthesis, and discusses some of the underlying phenomena and issues involved.

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Section: Methodsmentioning

confidence: 99%

Controlled Microwave Heating in Modern Organic Synthesis

2004

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“…Furthermore, the use of microwaves in lipase-catalyzed reactions has been increasingly studied during the past decade, leading to improvements in conversion, reaction rates, and stereoselectivities (20Á30). Moreover, Rejasse and co-workers demonstrated that the stability of immobilized lipase B from Candida antarctica (CAL B, Novozym 435) in organic media can be enhanced by using microwave dielectric heating rather than conventional thermal heating, thus explaining the enhancements observed in conversion rate (31,32).…”

Section: Research Lettermentioning

confidence: 99%

Microwave-assisted solvent-free lipase catalyzed transesterification of β-ketoesters

Risso

Mazzini

Kröger

et al. 2012

Green Chemistry Letters and Reviews

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Lipase-catalyzed transesterification was used as an efficient tool for the interconversion of b-ketoesters. Catalytic activity of commercial lipase B from Candida antarctica (Novozym 435) was evaluated in systems involving non activated acyl donors, and enhanced using microwave irradiation. Interestingly, the combination of CAL B in microwave irradiation worked excellent in solvent-free conditions, thus assuring a highly competitive and environment-friendly process with high yields (up to 96%) in competitive times (B 2h). The combination of biocatalysis with solvent-free systems and microwave assistance is currently scarcely used, and may represent a powerful synergy for preparative reactions.

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“…As an example, these effects are used in biocatalysis discipline (Réjasse et al, 2004;2006;Yadav & Borkar, 2009;Souza et al, 2009), DNA hybridization (Edwards, Young, & Deiters, 2009) and the studies that evaluate enzyme stability with the use of microwave irradiation (Rejasse et al, 2007;Young et al, 2008;Izquierdo et al, 2007;Porcelli et al, 1997;La Cara et al, 1999). Recently, Laurence et al (2000), H. Bohr and J. Bohr (2000), Pomerai et al (2003) reported unfolding or structural changes in proteins with microwave irradiations and suggested the inclusion of www.ccsenet.org/ijc…”

Section: Applications In Proteomicsmentioning

confidence: 99%

Microwave Assisted Reactions in Organic Chemistry: A Review of Recent Advances

Jacob¹

2012

IJC

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Revolution in organic compound synthesis has been promoted by microwave assisted organic syntheses (MAOS) by which small molecules are built up into large polymers in a fraction of time. The need for different organic compound libraries for drug discovery, biomaterial development, automated library screening; proteomics etc has supported the emergence of innovative technologies for rapid combinatorial organic synthesis using MAOS synthesis. In previous reviews on this subject the focus of MAOS has been on the process of MAOS reactions rather than the importance given to the related applications. This review focuses on solid-phase synthesis, biopolymer synthesis, applications in proteomics, parallel processing in microwave reactors and automated library generation by means of sequential microwave irradiation methods. This article has discussed the different applications of Microwave assisted synthesis of organic polymeric compounds most thoroughly by focusing on aspects of speed, reproducibility and scalability. From this review it is clearly identified that independent on the type of organic material, data consistently points out to MW as a novel and powerful tool which has enable synthesis of a number of new compounds and presents the need for future research in this area.

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Stability improvement of immobilized Candida antarctica lipase B in an organic medium under microwave radiation

Cited by 69 publications

References 12 publications

Controlled Microwave Heating in Modern Organic Synthesis

Controlled Microwave Heating in Modern Organic Synthesis

Microwave-assisted solvent-free lipase catalyzed transesterification of β-ketoesters

Microwave Assisted Reactions in Organic Chemistry: A Review of Recent Advances

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