Stability-indicating high-performance thin-layer chromatography method for the simultaneous estimation of emtricitabine and tenofovir alafenamide fumarate

Kashid, Arun M.; Kadam, Rohini R.

doi:10.1007/s00764-021-00080-1

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…Aliquot a 20 mL stock solution of each of the aforementioned solutions into a 2 mL of solvent (diluent) that contains 10.00 Mcg/mL of each analyte (Kashid and Kadam, 2021).…”

Section: Preparation Of Workable Solutionsmentioning

confidence: 99%

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Development and Validation of UPLC-MS/MS Bioanalytical Method for Simultaneous Quantification of the Antiretroviral Drugs Dolutegravir, Lamivudine, and Tenofovir in Human Plasma

Vyshali,

Gandla

2023

IJPQA

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The current proposed validated bio-analytical UPLC-MS/MS method for assessing components like dolutegravir, lamivudine, and tenofovir in human plasma using bictigravir as an internal standard with a mobile phase of formic acid (0.1%), 2 Mm ammonium formate in water and acetonitrile (30:70, v/v), and optimized flow rate of 0.5 mL/min, the separation was performed on an Agilent XDB C18 column (250 mm X 4.6, 5μ). The three components were analyzed by using LC-MS/MS in positive ion mode owing to the presence of primary groups. For the technique validation for the concentration range of 20 to 1000ng/mL for dolutegravir, lamivudine, and tenofovir in human plasma, bitigravir was employed as the internal control. The three-drug moieties average recoveries from spiking plasma samples were discovered to be reproducible. Based on the data displayed above, it was determined that the procedure was rapid and dependable, with a minimum total run duration of 3.0 minutes. The stability studies were assessed as a consequence of the current approach’s successful validation in accordance with the food and drug administration (FDA), european medicines agency (EMA), and international council for harmonisation (ICH) requirements.

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“…Aliquot a 20 mL stock solution of each of the aforementioned solutions into a 2 mL of solvent (diluent) that contains 10.00 Mcg/mL of each analyte (Kashid and Kadam, 2021).…”

Section: Preparation Of Workable Solutionsmentioning

confidence: 99%

“…The completed literature review [1][2][3][4][5] results showed very few liquid chromatography tandem mass spectrometry (LC-MS/MS) techniques [6][7][8][9][10][11][12] for the drugs described above in human plasma (Grande et al, 2019). The current investigation was found to take less time and economic as a consequence.…”

Section: Introductionmentioning

confidence: 96%

Development and Validation of UPLC-MS/MS Bioanalytical Method for Simultaneous Quantification of the Antiretroviral Drugs Dolutegravir, Lamivudine, and Tenofovir in Human Plasma

Vyshali,

Gandla

2023

IJPQA

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show abstract

“…For these reasons, we developed a green micellar HPTLC method for the simultaneous assessment of the examined drug combination in pure forms and medicinal dosage formulations. HPTLC is an analytical technique that has been utilized to analyze a variety of pharmacological combinations [16][17][18][19][20][21][22] as well as stability-indicating processes [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Application of the green analytical procedure index to the simultaneous analysis of co-formulated tinidazole and ciprofloxacin in pure form, tablet dosage form, and human plasma using an environmentally friendly micellar high-performance thin-layer chromatographic technology

Saraya

Hassan

Eltoukhi

et al. 2023

JPC-J Planar Chromat

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In individuals with chronic refractory pouchitis and other gastrointestinal disorders, the combination of ciprofloxacin (CIP) and tinidazole (TIN) is beneficial and safe. As a result, a green micellar high-performance thin-layer chromatographic (HPTLC) approach for the immediate analysis of TIN and CIP in pure, spiked human plasma, and co-formulated tablet dosage form has been developed. It is rapid, extremely easy, sensitive, cost-effective, and environmentally friendly. The stationary phase was Merck aluminum HPTLC plates covered with silica gel 60 F254, while the mobile phase was acetone–ethanol–2% watery sodium dodecyl sulfate (3:4:2, V/V). For quantification of both medications, the densitometric scanner was set at 310 nm. For TIN and CIP, this chromatographic separation yielded symmetric, compact peaks with RF values of (0.22 ± 0.009) and (0.42 ± 0.007), respectively. At 310 nm, the separated spots were densitometrically scanned. For TIN and CIP, the detection thresholds were 6.7 ng/band and 25.03 ng/band, respectively. For TIN and CIP, the quantification limits were 20.3 ng/band and 75.25 ng/band, respectively. The approach was validated according to International Council for Harmonisation (ICH) principles and then used to determine the researched medicines in their various pharmaceutical dosage forms, and human plasma yielding an exceptional percent of recovery. In terms of precision and accuracy, the results were in great accordance with the published approach. This method is suitable for the sequential analysis of the two drugs in pure form, tablet dosage forms, and spiked human plasma due to its simplicity, speed, greenness, robustness, and low cost.

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Stability-indicating high-performance thin-layer chromatography method development and validation for dapagliflozin propanediol monohydrate, vildagliptin, and metformin hydrochloride in active pharmaceutical ingredient and its tablet dosage form

Kashid,

Shinde,

Pere

et al. 2024

JPC-J Planar Chromat

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Stability-indicating high-performance thin-layer chromatography method for the simultaneous estimation of emtricitabine and tenofovir alafenamide fumarate

Cited by 5 publications

References 5 publications

Development and Validation of UPLC-MS/MS Bioanalytical Method for Simultaneous Quantification of the Antiretroviral Drugs Dolutegravir, Lamivudine, and Tenofovir in Human Plasma

Development and Validation of UPLC-MS/MS Bioanalytical Method for Simultaneous Quantification of the Antiretroviral Drugs Dolutegravir, Lamivudine, and Tenofovir in Human Plasma

Application of the green analytical procedure index to the simultaneous analysis of co-formulated tinidazole and ciprofloxacin in pure form, tablet dosage form, and human plasma using an environmentally friendly micellar high-performance thin-layer chromatographic technology

Stability-indicating high-performance thin-layer chromatography method development and validation for dapagliflozin propanediol monohydrate, vildagliptin, and metformin hydrochloride in active pharmaceutical ingredient and its tablet dosage form

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