Stability-indicating HPLC-DAD method development, validation, and stress degradation studies for triamterene and xipamide in their combined tablet dosage form

El-Kimary, Eman I.

doi:10.1556/achrom.28.2016.1.7

Cited by 15 publications

(10 citation statements)

References 17 publications

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“…The validated method had lower solvent consumption, replaced dangerous solvents by safer one and considered economic selection criteria for industrial establishments. The proposed method is eligible for daily routine simple use in different GAPI pictograms for previously reported methods; A: [18], B: [19], and C: the proposed method for determination of XIP and TRM. The red zones represent high ecological impact, yellow zones represent lower impact, and green zones represent safe effect to environment…”

Section: Resultsmentioning

confidence: 99%

“…Analytical application. Simultaneous determination of TRM and XIP in their tablet dosage form was applied by the proposed method, Epitens®, and results obtained were compared to results obtained from a previously reported method [18]. Student t-test and F-values (Table 4) were calculated and compared.…”

Section: Robustness Validated Chromatographic Conditionsmentioning

confidence: 99%

“…Green color symbolizes low ecological impact, while yellow and red colors represent higher impacts. GAPI was used to compare the proposed method to the other reported methods [18,19] ( Fig. 3).…”

Section: Evaluation Of the Analytical Proceduresmentioning

confidence: 99%

“…Both reported methods brought for comparison utilized ACN as organic modifier at percentage 50% and higher. Abd-Elhay [19] et al, proposed a superior isocratic technique over El-Kimary [18] who proposed a gradient elution technique. However, for both methods, the amount of ACN used and amount of generated waste taken along with its biohazards and high price may be less favorable than MeOH in the proposed study.…”

Section: Evaluation Of the Analytical Proceduresmentioning

confidence: 99%

“…So, the elution time of our drugs under study was quite longer. Only three methods were reported focusing on determination of the drugs under study using HPLC-UV detection [17][18][19]. Two of those methods have the disadvantage of using gradient technique with run time exceeding 12 min.…”

Section: Introductionmentioning

confidence: 99%

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An eco-friendly micellar HPLC method for the simultaneous determination of triamterene and xipamide in active pharmaceutical ingredients and marketed tablet dosage form

Ayad

Hosny

Ibrahim

et al. 2020

AChrom

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Abstract In the last few years, the use of surfactants as mobile phase additives in reversed phase liquid chromatography (RPLC) has been steadily developing and improving. Surfactants modify the polarity of the stationary phase which in turn decreases the amount of organic solvent required for elution of the analytes rendering the methodologies linked to them greener and more eco-friendly. Brij-35 is a fatty alcohol ethoxylates non ionic surfactant, which is less widely used as mobile phase additive. Brij-35 can decrease stationary phase polarity while remaining neutral. In this research, Brij-35 was studied in the separation and determination of marketed antihypertensive combination therapy composed of triamterene (TRM) and xipamide (XIP). TRM and XIP are diuretics used for treatment of essential hypertension and associated edema conditions. Chromatographic separation was achieved on RP-C18 column (Kinetix®, 5 µm, 15 cm × 4.6 mm) at flow rate 1 mL min−1 and UV-detection at 254 nm. Isocratic elution was performed using mobile phase composed of 0.1 M Brij-35: methanol (MeOH) (60:40, v/v). The analytes were well separated and quantified within linearity ranges of 5–50 µg mL−1 for both drugs in short retention time (2.6 and 5.3 min. for TRM and XIP, respectively). Since claiming greenness is not enough, Green Analytical Procedure Index (GAPI) was used to demonstrate the superiority of the proposed method over the previously reported methods. GAPI is a new metric for evaluation of the ecological impact of analytical procedures. The proposed method was validated according to ICH guidelines and applied successfully for simultaneous determination of the drugs in their co-formulated tablets.

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Section: Resultsmentioning

confidence: 99%

Section: Robustness Validated Chromatographic Conditionsmentioning

confidence: 99%

Section: Evaluation Of the Analytical Proceduresmentioning

confidence: 99%

Section: Evaluation Of the Analytical Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An eco-friendly micellar HPLC method for the simultaneous determination of triamterene and xipamide in active pharmaceutical ingredients and marketed tablet dosage form

Ayad

Hosny

Ibrahim

et al. 2020

AChrom

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Validated spectroscopic methods for the estimation of triamterene in bulk powder and capsule form via derivatization reactions

et al. 2018

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Sensitive, simple and specific spectrophotometric and spectrofluorimetric methods were developed for the determination of triamterene in bulk powder and pharmaceutical dosage form. The spectrophotometric method (Method A) is based on the formation of an ion‐pair complex with eosin Y in acetate buffered solution of pH 3.7 followed by measuring the absorbance at 545 nm. The absorbance–concentration plot is rectilinear over the range 3.0–15.0 μg/mL with a limit of detection (LOD) of 0.429 μg/mL and a limit of quantitation (LOQ) of 1.031 μg/mL. The spectrofluorimetric method (Method B) is based on the reaction of triamterene with 7‐ chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl) in basic solution (pH 10) to form a product with high fluorescence measured at 546 nm after being excited at 438 nm. The plot of fluorescence versus concentration is linear within the range 2.0–10.0 μg/mL. The suggested methods were applied for the analysis of commercial capsules containing the studied drug with successful results. The results obtained from the proposed method were statistically compared with those of a reported one and revealed good agreement. The presented methods are useful in routine analysis of triamterene in laboratories of quality control.

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Sensitive inexpensive chromatographic determination of an antimicrobial combination in human plasma and its pharmacokinetic application

El-Kimary

Khamis

Belal

et al. 2018

Journal of Chromatography B

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Stability-indicating HPLC-DAD method development, validation, and stress degradation studies for triamterene and xipamide in their combined tablet dosage form

Cited by 15 publications

References 17 publications

An eco-friendly micellar HPLC method for the simultaneous determination of triamterene and xipamide in active pharmaceutical ingredients and marketed tablet dosage form

An eco-friendly micellar HPLC method for the simultaneous determination of triamterene and xipamide in active pharmaceutical ingredients and marketed tablet dosage form

Validated spectroscopic methods for the estimation of triamterene in bulk powder and capsule form via derivatization reactions

Sensitive inexpensive chromatographic determination of an antimicrobial combination in human plasma and its pharmacokinetic application

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