Stability Indicating HPLC Method for Simultaneous Estimation of Entacapone, Levodopa and Carbidopa in Pharmaceutical Formulation

Bhatnagar, Prasoon; Vyas, Disha J.

doi:10.4172/2157-7064.1000304

Cited by 13 publications

(1 citation statement)

References 7 publications

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“…Repeatability, establishes the precision of multiple sampling under the same operating conditions over a short interval time, while intermediate precision establishes variations from the same laboratory under variable operation conditions, for example different days, analysts or equipment. Hence, the method was found precise (19).…”

Section: Precisionmentioning

confidence: 95%

Evaluatıon Of Release Of L-DOPA From PLGA Nanopartıcles Wıth Dıfferent In Vıtro Relase Methods By An Optımızed HPLC Method

Arısoy¹,

Sayiner²,

Çomoğlu³

2020

Bezmialem Science

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Amaç: Farmasötik alanda çözünme hızı testleri, ilaç ürün performansını karakterize etmek için önemli parametrelerdendir. Üretim sürecinden sonra ürün kalitesini ve performansını sağlamak için, formülasyondaki değişiklikler, yeni formülasyonların geliştirilmesi sırasında çözünme testleriyle değerlendirilebilir. Yöntemler: Bu çalışmada, poli (D, L-laktik-ko-glikolik asit) (PLGA) nanopartiküllerinden salınan L-Dopa miktarının belirlenmesi için Yüksek Basnıçlı Sıvı Kromatografisi (HPLC) yöntemi geliştirilmiş ve onaylanmıştır. 280 nm dalga boyunda hareketli faz olarak % 0.05 trifloroasetik asit (5:95, h/ h) içeren asetonitril-su kullanılarak, ters fazlı C18 kolonu ile kromatografik ayırma yapılmıştır. Geliştirilen yöntem, özgünlüğü, doğrusallığı, doğruluğu ve kesinliği için ICH kurallarına göre doğrulanmıştır. Bulgular: Yöntemin 1.25-40 µg / mL konsantrasyon aralığında doğrusal (r2 ≥ 0,995) olduğu gösterilmiştir. Ortalama % geri kazanım, % 102.59-% 98.70 arasında olup, gerçek değer ile tespit edilen değer arasında bir korelasyon olduğu belirlenmiştir. Antioksidan ilavesiyle çözelti stabilitesi sağlanmıştır. Analitik yöntemin nanopartiküllerden salınan L-Dopa'nın değerlendirilmesi için uygun olduğu gösterilmiştir. Örnek ve ayırma (SS) ve diyaliz membranı (DM) yöntemleri kullanılarak in vitro salım çalışmaları yapılmıştır. SS ve DM yöntemlerinin karşılaştırılmasında farklılık (ƒ1) ve benzerlik (ƒ2) faktörleri kullanılmıştır. L-Dopa salım kinetiğindeki fark her iki yöntem için de anlamlı bulunmamıştır (ƒ1 < 15 ve ƒ2 > 50). Objective: Dissolution tests have emerged in the pharmaceutical field as a very important tool to characterize drug product performance. To assure product quality and performance after the manufacturing process, changes in the formulation can be assessed through dissolution tests during the development of new formulations. Methods: In this study, a simple High Performance Liquid Chromatography (HPLC) method was developed and validated for the quantitation of release of L-Dopa from poly (D, L-lactic-coglycolic acid) (PLGA) nanoparticles. Chromatographic separation was done with a reversed-phase C18 column, using acetonitrilewater containing 0,05% trifluoroacetic acid (5:95, v/v) as a mobile phase at a wavelength of 280 nm. The developed method was validated according to ICH guidelines for its specificity, linearity, accuracy, and precision. Results:The method was shown to be linear (r2 ≥ 0.995) over the concentration range of 1,25-40 µg / mL. The mean % recoveries were in between, 102.59 %-98.70 % indicating an agreement between the true value and the detected value. Solution stability was guaranteed with antioxidant addition. The analytical method was shown to be suitable for the evaluation of L-Dopa which is released from nanoparticles. In vitro release studies were performed using sample and separate (SS), and dialysis membrane (DM) methods. Difference (ƒ1) and similarity (ƒ2) factors were used in the comparison of the SS and DM methods. The difference in the

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Section: Precisionmentioning

confidence: 95%

Evaluatıon Of Release Of L-DOPA From PLGA Nanopartıcles Wıth Dıfferent In Vıtro Relase Methods By An Optımızed HPLC Method

Arısoy¹,

Sayiner²,

Çomoğlu³

2020

Bezmialem Science

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RP-HPLC Method development, Validation and Forced Degradation for Simultaneous estimation of Benserazide HCl and Levodopa in a Marketed Formulation

Bhoir¹,

Rao²

2020

IJPTR

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The objective of the study was to develop and validate a novel, stability indicating, simple, rapid, accurate, precise and isocratic reverse-phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of benserazide HCl and levodopa in a marketed formulation. Chromatographic separation was achieved by using C18 Cosmosil 4.6 × 250 mm column with a mixture of phosphate buffer pH 2 and acetonitrile in proportion of 95:5 as mobile phase at a flow rate of 1.0 ml/min and column temperature 25°C. The detection was carried out at 210 nm using UV detector. The retention time for benserazide and levodopa was found to be 3.1 minutes and 6.6 minutes respectively and recoveries from tablet were between 98 and 102 %.

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An Examination of Quantum Information Processing Through Quantum Cryptography; A study

Chinnasamy¹,

Ramachandran²,

Murugan³

2022

jacp

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"Along with these developments, personal microwave technology has enabled strong non-linear effects at the photon level, leading to readily observable novel parameter regimes in quantum optics. Circuit QED has opened up new opportunities to explore the rich physics of quantum information processing (QIP) and quantum optics (QO), making them scalable on the road to quantum computing. However, we must also discuss some of the challenges involved. Quantum Technologies (QT) is a cross-disciplinary field that has made great progress in recent years. Technologies that can explicitly represent individual quantum states, as well as superposition and entanglement, are now being developed to exploit the 'strange' properties of quantum mechanics. In quantum communication, individual or entangled photons are used to securely send data, while quantum simulation utilizes well-controlled quantum systems that are less accessible. Interest is growing in higher dimensional quantum states and quantum communication, as the extended availability of Hilbert space and greater information capacity, along with increased noise elasticity, offer many advantages and new research possibilities. Let's focus our attention on the benefits of higher dimensional quantum states for quantum communication, as shown by Kuditz and others. Nevertheless, it has been demonstrated that higher dimensional quantum states can also provide improvements in many other areas."

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Stability Indicating HPLC Method for Simultaneous Estimation of Entacapone, Levodopa and Carbidopa in Pharmaceutical Formulation

Cited by 13 publications

References 7 publications

Evaluatıon Of Release Of L-DOPA From PLGA Nanopartıcles Wıth Dıfferent In Vıtro Relase Methods By An Optımızed HPLC Method

Evaluatıon Of Release Of L-DOPA From PLGA Nanopartıcles Wıth Dıfferent In Vıtro Relase Methods By An Optımızed HPLC Method

RP-HPLC Method development, Validation and Forced Degradation for Simultaneous estimation of Benserazide HCl and Levodopa in a Marketed Formulation

An Examination of Quantum Information Processing Through Quantum Cryptography; A study

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