Stability of Blood Gases in Ice and at Room Temperature

Use of Pericardial Fluid pH to Distinguish between Idiopathic and Neoplastic Effusions

“…The results showed pH, pO 2 , and pCO 2 to be surprisingly resistant to clinically relevant changes for up to 30-60 minutes at room temperature. [23][24][25] Whereas our data suggest that supernatant pH from PE cases ideally should be measured within 30 minutes of collection, capped samples kept at room temperature can safely be assumed to be clinically stable for up to 60 minutes.…”

Changes in gas composition and acid‐base values of venous blood samples stored under different conditions in 4 domestic species

“…The effect of storage temperature and time on blood gas and acid-base values has been investigated intensively. [1][2][3] In humans and cattle, changes in acid-base values have been shown to result from delays in the time to analysis and variations in storage temperature. 4,5 In venous blood samples from cattle, storage time and temperature significantly altered bicarbonate concentration and pH, with decreases in these analytes in samples stored at 378C.…”

“…6 This result has been attributed to rapid anaerobic metabolism as a consequence of the high storage temperature. 2 The type of syringe used for sampling also may influence in vitro changes in PO 2 : the diffusion of oxygen through the plastic wall of the syringe increases PO 2 , whereas the aerobic metabolism of leukocytes can decrease PO 2 at high storage temperature. 2,4,7 In bovine samples stored at 228C and 48C for up to 12 and 48 hours, respectively, acid-base values did not change and could be used for clinical purposes.…”

“…2 The type of syringe used for sampling also may influence in vitro changes in PO 2 : the diffusion of oxygen through the plastic wall of the syringe increases PO 2 , whereas the aerobic metabolism of leukocytes can decrease PO 2 at high storage temperature. 2,4,7 In bovine samples stored at 228C and 48C for up to 12 and 48 hours, respectively, acid-base values did not change and could be used for clinical purposes. 6 The aim of this study was to evaluate the effect of time delay and storage temperature on acid-base values and to assess species-specific differences.…”

Glass syringes are better than plastic for preserving arterial blood gas for oxygen partial pressure determination: an explanation based on nanomaterial composition

“…Delay in analysis can decrease oxygen partial pressure (PO 2 ) and increase carbon dioxide partial pressure (PCO 2 ) because of the metabolism of blood cells. Ice preservation is recommended; however, there is no reason to keep arterial blood in ice if the blood gas analysis is done within 30 minutes (Liss and Payne 1993). The effect of syringe material on collected blood in general clinical chemistry has also been reported, including the diffusion of chemicals across the tube (Hilty et al 1969; Scott et al 1971).…”