Stability of Delta-9- Tetrahydrocannabinol in Stored Blood and Serum

Wong, Alexander; Orbanosky, M W; Reeve, V C; Beede, Jennifer

doi:10.1037/e497082006-012

Cited by 14 publications

(12 citation statements)

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“…Compounds with similar structures did not interfere with accurate THCCOOH quantitation. Some studies on the short-and long-term stability of THC [23][24][25][26][27] and THCCOOH [23,25,27] have been published in blood, serum and plasma. However, the stability of cannabinoids in hair samples has rarely been addressed [22].…”

Section: Discussionmentioning

confidence: 99%

Simultaneous analysis of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography–tandem mass spectrometry

Han

Park

Kim

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

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Section: Discussionmentioning

confidence: 99%

Simultaneous analysis of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography–tandem mass spectrometry

Han

Park

Kim

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

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“…Apart from analytical imprecision, decreases in OF cannabinoid concentrations over time could be caused by irreversible cannabinoid binding to surfaces and/or precipitants (27), and/or by enzymatic degradation (28); >97% of OF is water, but electrolytes, immunoglobulins, enzymes, and other proteins are present (29-30). Many hormones and enzymes present in plasma were detected in OF, albeit in lower concentrations (31).…”

Section: Discussionmentioning

confidence: 99%

Cannabinoid Stability in Authentic Oral Fluid after Controlled Cannabis Smoking

et al. 2012

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BACKGROUND Defining cannabinoid stability in authentic oral fluid (OF) is critically important for result interpretation. There are few published OF stability data, and of those available, all employed fortified synthetic OF solutions or elution buffers; none included authentic OF following controlled cannabis smoking. METHODS An expectorated OF pool and a pool of OF collected with Quantisal™ devices were prepared for each of 10 participants. Δ9-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) stability in each of 10 authentic expectorated and Quantisal-collected OF pools were determined after storage at 4 °C for 1 and 4 weeks and at −20 °C for 4 and 24 weeks. Results within ±20% of baseline concentrations analyzed within 24 h of collection were considered stable. RESULTS All Quantisal OF cannabinoid concentrations were stable for 1 week at 4 °C. After 4 weeks at 4 °C, as well as 4 and 24 weeks at −20 °C, THC was stable in 90%, 80%, and 80% and THCCOOH in 89%, 40%, and 50% of Quantisal samples, respectively. Cannabinoids in expectorated OF were less stable than in Quantisal samples when refrigerated or frozen. After 4 weeks at 4 and −20 °C, CBD and CBN were stable in 33%–100% of Quantisal and expectorated samples; by 24 weeks at −20 °C, CBD and CBN were stable in ≤44%. CONCLUSIONS Cannabinoid OF stability varied by analyte, collection method, and storage duration and temperature, and across participants. OF collection with a device containing an elution/stabilization buffer, sample storage at 4 °C, and analysis within 4 weeks is preferred to maximize result accuracy.

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“…For efficient transcorneal permeation, the therapeutic agents must possess optimum hydrophilic and hydrophobic characteristics (1). Moreover, adsorption of cannabinoids to glass and plastics poses a significant challenge in formulation, analysis, and topical delivery of these drugs (21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Solubility, Stability, and Transcorneal Permeability of Delta-8-Tetrahydrocannabinol in the Presence of Cyclodextrins

et al. 2011

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Abstract. The purpose of this study was to investigate the effect of cyclodextrins (CDs) on aqueous solubility, stability, and in vitro corneal permeability of delta-8-tetrahydrocannabinol (Δ 8 -THC). Phase solubility of Δ 8 -THC was studied in the presence of 2-hydroxypropyl-β-cyclodextrin (HPβCD), randomly methylated-β-cyclodextrin (RMβCD) and sulfobutyl ether-β-cyclodextrin sodium salt (SβCD). Stability of Δ 8 -THC in 5% w/v aqueous CD solutions, as a function of pH, was studied following standard protocols. In vitro corneal permeation of Δ 8 -THC (with and without CDs) across excised rabbit cornea was also determined. Phase-solubility profile of Δ 8 -THC in the presence of both HPβCD and RMβCD was of the A P type, whereas, with SβCD an A L type was apparent. Aqueous solubility of Δ 8 -THC increased to 1.65, 2.4, and 0.64 mg/mL in the presence of 25% w/v HPβCD, RMβCD, and SβCD, respectively. Significant degradation of Δ 8 -THC was not observed within the study period at the pH values studied, except for at pH 1.2. Transcorneal permeation of Δ 8 -THC was dramatically improved in the presence of CDs. The results demonstrate that CDs significantly increase aqueous solubility, stability, and transcorneal permeation of Δ 8 -THC. Thus, topical ophthalmic formulations containing Δ 8 -THC and modified beta CDs may show markedly improved ocular bioavailability.

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Stability of Delta-9- Tetrahydrocannabinol in Stored Blood and Serum

Cited by 14 publications

References 0 publications

Simultaneous analysis of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography–tandem mass spectrometry

Simultaneous analysis of Δ9-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol in hair without different sample preparation and derivatization by gas chromatography–tandem mass spectrometry

Cannabinoid Stability in Authentic Oral Fluid after Controlled Cannabis Smoking

Enhanced Solubility, Stability, and Transcorneal Permeability of Delta-8-Tetrahydrocannabinol in the Presence of Cyclodextrins

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