Stability of dobutamine 500 mg in 50 ml syringes prepared using a Central Intravenous Additive Service

Patel, Nilesh; Taki, Mohammed; Tunstell, Paul; Forsey, Paul; Forbes, Ben

doi:10.1136/ejhpharm-2011-000027

Cited by 7 publications

(7 citation statements)

References 13 publications

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“…No loss in the concentration of dobutamine or appearance of degradation peaks could be observed after exposure of dobutamine solution to 0.5 mol/L hydrochloric acid for 72 hours. Our findings are consistent with the result reported by Patel et al 9 They stressed dobutamine in the presence of 0.1, 1 or 2 mol/L HCl for 72 hours and reported no degradation of dobutamine. The observed degradation products did not interfere with the peak of dobutamine, suggesting the developed HPLC method is capable of investigating the chemical stability of dobutamine.…”

Section: Stability-indicating Capability Of the Newly Developed Hplsupporting

confidence: 94%

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Stability of dobutamine in continuous ambulatory delivery devices

et al. 2018

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Section: Stability-indicating Capability Of the Newly Developed Hplsupporting

confidence: 94%

“…8 The stability of dobutamine in various fluids and containers has been investigated before. For example, Patel et al 9 for 24 hours. Also, dobutamine is known to be stable for 48 hours when mixed with large volume parenteral solutions.…”

Section: Discussionmentioning

confidence: 99%

Stability of dobutamine in continuous ambulatory delivery devices

et al. 2018

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“…Exposure to light is thought to be one of the dominating stability factors [14,15,22], however in our study differentiation in the stability of inotropes in varying dilution vehicles was not seen at location A after 24 hours. More importantly we did not observe any significant differences in the concentration of dopamine in 0.9 % NaCl at location A and B after 24 hours in clear and orange tubing (utilised to present light).…”

Section: Differences In the Stability Of Inotropes Following 24-hour contrasting

confidence: 79%

“…One potential explanation of this observation is due to the stability of the drug. There are a limited number of studies conducted on the stability of dopamine and dobutamine which have found that light and temperature appear to be dominating factors for influencing stability [11][12][13][14][15][16]. However, most these studies have not been conducted on clinical doses and in environments that match ward settings.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Stability of Dopamine and Dobutamine Over 24 h in Simulated Neonatal Ward Conditions

et al. 2017

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Objectives. Our objectives were to investigate the possible effects temperature and light have on the stability of dopamine and dobutamine continuous infusions over 24 hours when prepared in varying dilution vehicles.Methods Syringe-driver infusion apparatuses were set up of either dopamine or dobutamine, diluted with either 0.9% sodium chloride or 5% glucose, delivering 3mcg/kg/min and 5mcg/kg/min respectively via 206 cm extension sets. All infusions were prepared for a neonate weight of 1 kg. Infusions were run over 24 hours, where approximately half the tubing was within an incubator set at 35ᵒC. Cyclic voltammetry was utilised to monitor the initial concentration of the inotrope and that after 24 hours within the syringe and end of the extension set.Results. The variation in the concentration of dopamine and dobutamine in the vials was 3.58 % and 1.22 % respectively (n=6). This variation in the concentration increased to 10.88 % for dopamine and 5.76 % for dobutamine in preparation of the syringe. A significant reduction in the concentration of dopamine was observed at the end of the extension set after 24 hours when prepared in 0.9 % NaCl when compared to dopamine prepared in 5 % glucose (p<0.001, n=6-7) or dobutamine prepared in 0.9 % NaCl (p<0.001, n=6-7). No differences in the concentration of dopamine in 0.9 % NaCl were observed after 24 hours in light-exposed and light-protected extension sets (n=6-7).2 Conclusions. Dobutamine is more stable in dilution vehicles when compared to dopamine and inotropes are more stable in 5 % glucose dilution vehicle when compared to 0.9 % NaCl. Such findings will provide guidance on the choice of inotropes. Key points• Inotropes such as dopamine and dobutamine are commonly utilised to treat neonatal hypotension and are administered as a continuous infusion over 24 hours• Under experimental conditions that mimicked the local neonatal unit environment, dopamine prepared in 0.9 % sodium chloride was significantly less stable than that prepared in 5 % glucose over 24 hours. Dopamine was also significantly less stable than dobutamine over 24-hours when prepared in 0.9 % sodium chloride.• Clinical staff might want to consider the results of our study, as our findings indicate that the stability of inotropes vary specifically in the dilution vehicle, of which 5% glucose is the preferred dilution vehicle.3

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“…Several methods have been reported for the analyses of this compound, such as spectrophotometric assay [6] , pH metric determination [7] , chemiluminescence [8] , HPLC assay with fluorimetric, electrochemical and UV detection [9] , [10] , [11] , [12] , [13] , [14] and solid phase extraction method [15] .The present paper reports on the development of a new, validated, fast and sensitive method by using HPLC with PDA detection for the quantitative determination of DBT in rat plasma. Compared with old methods this is more sensitive, rapid and validated at very low level of quality control (QC) samples.…”

Section: Introductionmentioning

confidence: 99%

Optimization and validation of a fast RP–HPLC method for the determination of dobutamine in rat plasma: Pharmacokinetic studies in healthy rat subjects

Ramesh

Pothuraju

Ramisetti

et al. 2013

Journal of Pharmaceutical Analysis

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A novel isocratic reverse phase high performance liquid chromatography (RP–HPLC) with photo diode array (PDA) detection method for the determination of dobutamine (DBT) in rat plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Homoveratrylamine was used as an internal standard. Methanol was used as the extracting solvent for the preparation of plasma samples. Samples were separated on a Symmetry C18 (250 mm×4.6 mm i.d., 5 μm) analytical column. Acetonitrile and 15 mM potassium dihydrogen phosphate (pH 5.0 with 0.3% TEA) (20:80, v/v) was used. The column oven temperature was optimized at 35 °C and the flow rate was 0.8 mL/min. The detection wavelength was fixed at 230 nm for entire analysis. The calibration curve was found to be linear over the concentration range of 50–2000 ng/mL (r2=0.9992). The limit of quantification (LOQ) of the method was 50 ng/mL. The % RSD values of accuracy and precision values for intra and inter days were <15% at quality control (QC) concentrations. Recovery, stability and robustness were studied within the acceptable range according to ICH guidelines. The method was efficiently applied to a pharmacokinetic study in healthy Wistar rats.

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Stability of dobutamine 500 mg in 50 ml syringes prepared using a Central Intravenous Additive Service

Cited by 7 publications

References 13 publications

Stability of dobutamine in continuous ambulatory delivery devices

Stability of dobutamine in continuous ambulatory delivery devices

Understanding the Stability of Dopamine and Dobutamine Over 24 h in Simulated Neonatal Ward Conditions

Optimization and validation of a fast RP–HPLC method for the determination of dobutamine in rat plasma: Pharmacokinetic studies in healthy rat subjects

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