Low Velocity Zones (LVZs) with anomalously high V p -V s ratios occur along the downdip extents of subduction megathrusts in most modern subduction zones and are collocated with complex seismic and transient deformation patterns. LVZs are attributed to high pore fluid pressures, but the spatial correlation between the LVZ and the subduction interface, as well as the rock types that define them, remain unclear. We characterize the seismic signature of a fossil subduction interface shear zone in northern California that is sourced from the same depth range as modern LVZs. Deformation was distributed across 3 km of dominantly metasedimentary rocks, with periodic strain localization to km-scale ultramafic lenses. We estimate seismic velocities accounting for mineral and fracture anisotropy, constrained by microstructural observations and field measurements, resulting in a V p /V s of 2.0. Comparable thicknesses and velocities suggest that LVZs represent, at least in part, the subduction interface shear zone.Plain Language Summary Many subduction zones -places where one tectonic plate goes under another -have areas where seismic waves travel up to three times slower than normal and where the ratio of speeds of two different types of seismic waves is anomalously high. Some researchers have concluded that these Low Velocity Zones (LVZs) at 25-50 km below the surface of the Earth are the undeformed top of a downgoing tectonic plate whereas others suggest that LVZs are zones of intense deformation that allow two tectonic plates to slide past each other. To help resolve this uncertainty, we investigated rocks in a fossil subduction zone that record a history of being subducted and then returned to the surface. We identified the thickness of a zone of deformation and estimated how fast seismic waves would have passed through this zone based on the rock types, how the minerals are oriented, and the presence of fractures, all of which affect seismic speeds. The thicknesses and seismic wave speeds are comparable to modern LVZs, suggesting that LVZs mark zones of deformation between tectonic plates. These results can help us better understand how plates move past each other in modern subduction zones.