Stability of fluorescent labels in PLGA polymeric nanoparticles: Quantum dots versus organic dyes

Abdel-Mottaleb, Mona M.A.; Béduneau, Arnaud; Pellequer, Yann; Lamprecht, Alf

doi:10.1016/j.ijpharm.2015.08.050

Cited by 39 publications

(21 citation statements)

References 30 publications

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“…Our release study confirms the literature data with polymeric NC versus NE, where sustained release of lipophilic compounds is achieved by the polymeric wall of NC and drug retention is governed by partition between the oily core and the continuous aqueous phase (Cruz et al, 2006;Mosqueira et al, 2006). The use of some organic fluorescent dyes such as Nile Red or Rhodamine B as NP markers is criticized on the basis of their leakage from the NP (Abdel- Mottaleb et al, 2015). In addition to the in vitro release study, a preliminary test was carried out to probe fluorescence transfer towards cells in cell culture medium via leakage from the NC.…”

Section: Dye Encapsulation and Releasesupporting

confidence: 82%

Impact of dose and surface features on plasmatic and liver concentrations of biodegradable polymeric nanocapsules

Oliveira

Paula

Roatt

et al. 2017

European Journal of Pharmaceutical Sciences

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Section: Dye Encapsulation and Releasesupporting

confidence: 82%

Impact of dose and surface features on plasmatic and liver concentrations of biodegradable polymeric nanocapsules

Oliveira

Paula

Roatt

et al. 2017

European Journal of Pharmaceutical Sciences

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“…[23–25] Because of their size-tuneable absorptions/emissions, and high fluorescence quantum yield, QDs are considered a better alternative to organic dyes. [21, 22] Although being widely used as fluorescent barcodes in platforms such as FACS[26, 27] and droplet microfluidics[28], there have been concerns regarding QDs’ cytotoxicity, with heavy metals being used in their crystal structure. [29] Rare earth (RE)-doped materials are a class of phosphors that have received attention for their potential applications in bio-imaging, sensing, and therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Luminescent nanomaterials for droplet tracking in a microfluidic trapping array

et al. 2018

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The use of high-throughput multiplexed screening platforms has attracted significant interest in the field of on-site disease detection and diagnostics for their capability to simultaneously interrogate single cell responses across different populations. However, many of the current approaches are limited by the spectral overlap between tracking materials (e.g., organic dyes) and commonly used fluorophores/biochemical stains, thus restraining their applications in multiplexed studies. This work demonstrates that the downconversion emission spectra offered by rare earth (RE)-doped β-hexagonal NaYF4 nanoparticles (NPs) can be exploited to address this spectral overlap issue. Compared to organic dyes and other tracking materials where the excitation and emission is separated by tens of nanometers, RE elements have a large gap between excitation and emission which results in their spectral independence from the organic dyes. As a proof of concept, two differently doped NaYF4 NPs (Europium: Eu3+ and Terbium: Tb3+) were employed on a fluorescent microscopy-based droplet microfluidic trapping array to test their feasibility as spectrally independent droplet trackers. The luminescence tracking properties of Eu3+-doped (red emission) and Tb3+-doped (green emission) NPs were successfully characterized by co-encapsulating with genetically modified cancer cell lines expressing green or red fluorescent proteins (GFP and RFP) in addition to a mixed population of live and dead cells stained with ethidium homodimer. Detailed quantification of the luminescent and fluorescent signals was performed to confirm no overlap between each of the NPs and between NPs and cells. Thus, the spectral independence of Eu3+-doped and Tb3+-doped NPs with each other and with common fluorophores highlights the potential application of this novel technique in multiplexed systems, where many such luminescent NPs (other doped and co-doped NPs) can be used to simultaneously track different input conditions on the same platform.

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“…Interaction of PLGA nanocarriers with cells is usually studied using fluorescent or radioactive labeling, which both suffer from poor spatial resolution. In addition, molecular leaching and photobleaching are serious limitations to conventional fluorescent labeling, whereas the use of radioactive labeling requires special handling and analysis. Alternatively, inorganic nanocrystals with fascinating optical, electronic, and magnetic properties have been used to label PLGA and other polymeric nanoparticles and thus allow for novel tracking and visualization experiments.…”

mentioning

confidence: 99%

“…PLGA is a hydrophobic polymer that dissolves in organic solvents . PLGA nanoparticles are typically prepared using two methods: 1) the nanoprecipitation method, in which PLGA is dissolved in a water‐miscible organic solvent such as acetone and then precipitated into nanoparticles upon addition into a nonsolvent (e.g., water); 2) the emulsion‐evaporation method, in which PLGA is dissolved in a water‐immiscible organic solvent such as dichloromethane, which is emulsified into an aqueous external phase followed by evaporation of the organic solvent to form PLGA nanoparticles . Obviously, efficient encapsulation requires dissolving both PLGA polymer and drug into the organic solvent (acetone or dichloromethane), and thus PLGA nanoparticles typically encapsulate hydrophobic rather than hydrophilic drugs.…”

mentioning

confidence: 99%

“…external phase followed by evaporation of the organic solvent to form PLGA nanoparticles. [6] Obviously, efficient encapsulation requires dissolving both PLGA polymer and drug into the organic solvent (acetone or dichloromethane), and thus PLGA nanoparticles typically encapsulate hydrophobic rather than hydrophilic drugs. With this in mind, and to encapsulate GNPs into PLGA nanoparticles, the former should be hydrophobic and exhibit an excellent colloidal stability in organic solvents.…”

mentioning

confidence: 99%

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Facile Functionalization of Gold Nanoparticles with PLGA Polymer Brushes and Efficient Encapsulation into PLGA Nanoparticles: Toward Spatially Precise Bioimaging of Polymeric Nanoparticles

Alkilany

Abulateefeh

Murphy

2018

Part & Part Syst Charact

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Nanocarriers prepared from poly(lactide‐co‐glycolide) (PLGA) have broad biomedical applications. Understanding their cellular uptake and distribution requires appropriate visualization in complex biological compartments with high spatial resolution, which cannot be offered by traditional imaging techniques based on fluorescent or radioactive probes. Herein, the encapsulation of gold nanoparticles (GNPs) into PLGA nanoparticles is proposed, which should allow precise spatial visualization in cells using electron microscopy. Available protocols for encapsulating GNPs into polymeric matrices are limited and associated with colloidal instability and low encapsulation efficiency. In this report, the following are described: 1) a facile protocol to functionalize GNPs with PLGA polymer followed by 2) encapsulation of the prepared PLGA‐capped GNPs into PLGA nanocarriers with 100% encapsulation efficiency. The remarkable encapsulation of PLGA‐GNPs into PLGA matrix obeys the general rule in chemistry “like dissolves like” as evident from poor encapsulation of GNPs capped with other polymers. Moreover, it is shown that how the encapsulated gold nanoparticles serve as nanoprobes to visualize PLGA polymeric hosts inside cancer cells at the spatial resolution of the electron microscope. The described methods should be applicable to a wide range of inorganic nanoprobes and provide a new method of labeling pharmaceutical polymeric nanocarriers to understand their biological fate at high spatial resolution.

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Stability of fluorescent labels in PLGA polymeric nanoparticles: Quantum dots versus organic dyes

Cited by 39 publications

References 30 publications

Impact of dose and surface features on plasmatic and liver concentrations of biodegradable polymeric nanocapsules

Impact of dose and surface features on plasmatic and liver concentrations of biodegradable polymeric nanocapsules

Luminescent nanomaterials for droplet tracking in a microfluidic trapping array

Facile Functionalization of Gold Nanoparticles with PLGA Polymer Brushes and Efficient Encapsulation into PLGA Nanoparticles: Toward Spatially Precise Bioimaging of Polymeric Nanoparticles

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