Stability of housekeeping genes in inflamed joints of spontaneous and collagen-induced arthritis in DBA/1 mice

Quiñonez-Flores, Celia María; López-Loeza, Salma Marcela; Pacheco‐Tena, César; Muñoz-Morales, Perla M; Acosta-Jiménez, Samara; González-Chávez, Susana Aideé

doi:10.1007/s00011-021-01453-2

Cited by 7 publications

(2 citation statements)

References 53 publications

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“…CIA was generated as described previously [ 24 ]. Briefly, DAB/1 mice were injected subcutaneously into the tail of 100 μL emulsion consisting of chicken type II collagen (100 μg) mixed with an equal volume of Complete Freund's Adjuvant (CFA).…”

Section: Methodsmentioning

confidence: 99%

Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment

Wang

Liu

et al. 2023

Materials Today Bio

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Section: Methodsmentioning

confidence: 99%

Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment

Wang

Liu

et al. 2023

Materials Today Bio

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“…RNA quantification was performed through RT-qPCR for Tnf , interleukin (Il)2 , Il6 , Il10 , Il12a , IL23a , transforming growth factor ( Tgfb1 ), and Janus kinase ( Jak ) 3 , with the primers sets listed in Table 1 . The ribosomal protein L ( Rpl ) 13 was used as the reference gene [ 24 ]. The cDNA was synthesized from total RNA (1µg) by RT using the SupeScript ® III First-Strand Synthesis System (Invitrogen by ThermoFisher Scientific) with random hexamer primers, and was then diluted to 100 µL.…”

Section: Methodsmentioning

confidence: 99%

Low-Intensity Physical Exercise Decreases Inflammation and Joint Damage in the Preclinical Phase of a Rheumatoid Arthritis Murine Model

et al. 2023

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Lifestyle modifications in preclinical Rheumatoid Arthritis (RA) could delay the ongoing pathogenic immune processes and potentially prevent its onset. Physical exercise (PE) benefits RA patients; however, its impact in reducing the risk of developing RA has scarcely been studied. The objective was to describe the effects of low-intensity PE applied at the disease’s preclinical phase on the joints of DBA/1 mice with collagen-induced arthritis (CIA). Twelve mice with CIA were randomly distributed into two groups: the CIA-Ex group, which undertook treadmill PE, and the CIA-NoEx, which was not exercised. The effects of PE were evaluated through clinical, histological, transcriptomics, and immunodetection analyses in the mice’s hind paws. The CIA-Ex group showed lower joint inflammation and damage and a decreased expression of RA-related genes (Tnf Il2, Il10, Il12a, IL23a, and Tgfb1) and signaling pathways (Cytokines, Chemokines, JAK-STAT, MAPK, NF-kappa B, TNF, and TGF-beta). TNF-α expression was decreased by PE in the inflamed joints. Low-intensity PE in pre-arthritic CIA reduced the severity through joint down-expression of proinflammatory genes and proteins. Knowledge on the underlying mechanisms of PE in preclinical arthritis and its impact on reducing the risk of developing RA is still needed.

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Identification of reliable reference genes for gene expression studies in mouse models under microplastics stress

Mu¹,

Wang²,

Wang³

et al. 2023

Ecotoxicology and Environmental Safety

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Stability of housekeeping genes in inflamed joints of spontaneous and collagen-induced arthritis in DBA/1 mice

Cited by 7 publications

References 53 publications

Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment

Treg-targeted efficient-inducible platform for collagen-induced arthritis treatment

Low-Intensity Physical Exercise Decreases Inflammation and Joint Damage in the Preclinical Phase of a Rheumatoid Arthritis Murine Model

Identification of reliable reference genes for gene expression studies in mouse models under microplastics stress

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