Stability of lactobacilli encapsulated in various microbial polymers

Jiménez-Pranteda, María Luján; Poncelet, Denis; Nader‐Macías, María Elena Fátima; Caruz, Antonio; Aguilera, Margarita; Monteoliva‐Sánchez, Mercedes; Ramos‐Cormenzana, A.

doi:10.1016/j.jbiosc.2011.10.010

Cited by 63 publications

(42 citation statements)

References 34 publications

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“…Our results were also found in similar with various researches which identified the chitosan as the best protective polymer compared to the others [2,20,22,24,30,49]. In addition, the size and shape of the capsules formed in this present work were found similar with capsules prepared in other studies employing the same methodology [18,31].…”

Section: Discussionsupporting

confidence: 91%

Effect of Encapsulation on Viability of Pediococcus pentosaceus OZF During Its Passage Through the Gastrointestinal Tract Model

2015

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The main goal of this study was to develop an improved oral delivery system for Pediococcus pentosaceus OZF, a promising probiotic bacterium, and to assess its viability under simulated gastrointestinal (GI) tract model by comparing the efficiency of microbiological and molecular approaches. Encapsulation was carried out using extrusion method and as a result, encapsulation system including 0.75 % lactulose, 1.8 % sodium alginate, 0.1 M CaCl2, and 5 min gelling time was shown to have a significantly protective effect against pH 2.0 acid stress over 3 h. However, completely loss of viability was exhibited by free OZF cells under similar conditions. To provide an additional barrier for capsules, coating process was investigated using different biopolymers, and the survival rates of free and encapsulated OZF cells upon expose to simulated GI conditions were detected by conventional culture techniques and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR) method. No significant differences between the biopolymers were detected, except the chitosan which leads totally 85 % protection and extra 25 % improvement in the survival of OZF cells compared to uncoated capsules. In conclusion, our findings indicated that chitosan-coated capsules provided an important protective effect on the viability of OZF cells against the GI system conditions encountered during the transit of food. In addition, this study was found successful in monitoring the viable OZF cells in capsules using PMA-qPCR method.

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Section: Discussionsupporting

confidence: 91%

Effect of Encapsulation on Viability of Pediococcus pentosaceus OZF During Its Passage Through the Gastrointestinal Tract Model

2015

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“…Surface morphological observations showed that Alg-Ch and Alg-XG yielded relatively smoother and more compact surface than Alg alone. Incorporation of XG instead of jamilan gum with the gellan gum system resulted in smoother and less porous bead surface [20]. On the other hand, Lee et al [21] highlighted that Alg particles coated with Ch were more spherical and smoother.…”

Section: Bead Sizementioning

confidence: 99%

Chitosan coated alginate–xanthan gum bead enhanced pH and thermotolerance of Lactobacillus plantarum LAB12

Fareez

Lim

Mishra

et al. 2015

International Journal of Biological Macromolecules

146

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“…Several studies on encapsulation of microorganisms with potential food application were carried out to protect them from adverse conditions in the gastrointestinal tract (Ding and Shah, 2009;Islam et al, 2010;Weinbreck et al, 2010;Jiménez-Pranteda et al, 2012). Only a few works reported the encapsulation of potential probiotic microorganisms for the human urogenital tract (Martín…”

Section: Discussionmentioning

confidence: 99%

“…Xanthan and gellan gums were employed to encapsulate L. reuteri CRL 1324 on the basis of the results obtained in other studies, in which different Lactobacillus and Bifidobacterium strains (for products administered orally) were successfully encapsulated with the aforementioned polymers (Sun and Griffiths, 2000;Jiménez-Pranteda et al, 2012).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Encapsulation and subsequent freeze-drying of Lactobacillus reuteri CRL 1324 for its potential inclusion in vaginal probiotic formulations

Tomás

Gregorio

Terraf

et al. 2015

European Journal of Pharmaceutical Sciences

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Probiotic formulations must include a high number of viable and active microorganisms. In this work, the survival of human vaginal Lactobacillus reuteri CRL 1324 during encapsulation, lyophilization and storage, and the activity of encapsulated and/or freeze-dried bacterial cells were evaluated. Extrusion-ionic gelation technique was applied to encapsulate L. reuteri CRL 1324, using xanthan and gellan. Encapsulated and free bacterial cells were freeze-dried with or without lactose and skim milk as lyoprotectors. The different systems obtained were stored at room temperature and at 4°C for 150days. The following determinations were performed: L. reuteri CRL 1324 viability, microorganism released from capsules, survival in a medium simulating the vaginal fluid and maintenance of beneficial properties (growth inhibition of opportunistic pathogenic Streptococcus agalactiae NH 17 and biofilm formation). L. reuteri CRL 1324 encapsulation was efficient, allowing the recovery of a high number of entrapped lactobacilli. The survival of encapsulated L. reuteri during lyophilization and storage was significantly higher in the presence of lyoprotectors. At the end of storage, the highest numbers of viable cells were obtained in free or encapsulated cells freeze-dried with lyoprotectors, stored at 4°C. Encapsulated and/or lyophilized L. reuteri cells maintained their viability in simulated vaginal fluid as well as the ability to inhibit S. agalactiae NH 17 growth and to form biofilm. Encapsulated and freeze-dried L. reuteri CRL 1324 can be included in a suitable pharmaceutical form for vaginal application to prevent or treat urogenital infections in women.

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Stability of lactobacilli encapsulated in various microbial polymers

Cited by 63 publications

References 34 publications

Effect of Encapsulation on Viability of Pediococcus pentosaceus OZF During Its Passage Through the Gastrointestinal Tract Model

Effect of Encapsulation on Viability of Pediococcus pentosaceus OZF During Its Passage Through the Gastrointestinal Tract Model

Chitosan coated alginate–xanthan gum bead enhanced pH and thermotolerance of Lactobacillus plantarum LAB12

Encapsulation and subsequent freeze-drying of Lactobacillus reuteri CRL 1324 for its potential inclusion in vaginal probiotic formulations

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