Stability of microencapsulated Lactobacillus acidophilus and Lactococcus lactis ssp. cremoris during storage at room temperature at low aw

Viability, Acid and Bile Tolerance of Spray Dried Probiotic Bacteria and Some Commercial Probiotic Supplement Products Kept at Room Temperature

“…lactis Bb12 and L. acidophilus 2401 (SD GM product) were then kept inside a desiccator containing saturated NaOH to achieve a w of 0.07. Once the equilibrium was achieved, the dehydrated products were stored in an aluminum foil pouch containing NaOH packed inside the semi‐permeable membrane to maintain the low a w of the product containing dried probiotics (Dianawati and others ). Storage was carried out at 25 °C for 10 wk.…”

“…Casein‐based emulsion combined with mannitol, glucose and fructooligosaccharides was used as microecapsulating materials, and spray drying was applied to create microsphere. This method maintained high survival of spray dried Bifidobacterium animalis after long period of storage at room temperature (Crittenden and others ; Dianawati and others ). In addition, the formulation was effective in protecting the probiotic bacteria from the harsh environment of gastric juice and bile.…”

“…We have developed a simple microencapsulation technology to produce dehydrated probiotics that can be stored for a long period of time without significantly affecting cell viability (Dianawati and others ). Casein‐based emulsion combined with mannitol, glucose and fructooligosaccharides was used as microecapsulating materials, and spray drying was applied to create microsphere.…”

Prebiotics as Protectants of Lactic Acid Bacteria

“…have been incorporated in coating materials for probiotic microencapsulation. Combining canola vegetable oil, caseinate, FOS, and resistant starch (portion of starch consisting in a linear molecule of α-1,4-d-glucan that resists digestion by human pancreatic amylase) highly improves the viability of probiotic strains after 5 weeks of storage in nonoptimal conditions (opened container at 25 °C and 50% relative humidity) (Crittenden et al, 2006;Dianawati et al, 2013). In turn, the incorporation of FOS into the coating materials improves the protection of Bifidobacterium bifidum during encapsulation (Chen et al, 2007).…”

“…In the last years, oligo-and polysaccharides including inulin, FOS, and GOS proved high efficiency as protectants of lactic acid bacteria (Schwab et al, 2007;Tymczyszyn et al, 2011Tymczyszyn et al, , 2012Golowczyc et al, 2013;Santos et al, 2014a,b). FOS are also efficient protectants of Lactobacillus acidophilus and Lactococcus cremoris during freeze-drying, spray-drying, and subsequent storage (Dianawati et al, 2013). Shu et al (2012) reported a protective effect of FOS, isomalto-oligosaccharides, inulin and xylo-oligosaccharides on freeze-dried Bifidobacterium bifidum.…”