Stability of paclitaxel-loaded solid lipid nanoparticles in the presence of 2-hydoxypropyl-β-cyclodextrin

Abstract

Paclitaxel (PTX)-loaded solid lipid nanoparticles without hydroxyl-β-cyclodextrin (PS) or with hydroxypropyl-β-cyclodextrin (PSC) were prepared by hot-melted sonication. Biocompatible and biodegradable stearic acid was used to produce the solid matrix. The stability of PS and PSC was assessed at different temperatures. Drug stability, as assessed by encapsulation efficiency (EE; %), particle size, and the polydispersity index (PDI), was examined and in vitro release of PTX from PS or PSC for up to 180 days was assessed. After 180 days of storage at 25 °C, no significant change in particle size, PDI, or EE of PS or PSC was observed. PS and PSC displayed similar sustained PTX release patterns. The particle size, PDI, EE, PTX release profile, and cytotoxicity of PS changed significantly with increasing incubation time, whereas those of PSC showed no significant change, when samples were stored at 40 ± 2 °C. PSC was more stable than PS in plasma with regard to particle size and PDI. These results demonstrate that PSC could be a promising formulation to increase drug stability.

Development of Houttuynia cordata Extract-Loaded Solid Lipid Nanoparticles for Oral Delivery: High Drug Loading Efficiency and Controlled Release

“…Solid lipid nanoparticles (SLNs) consist of an active compound dispersed in a melted solid lipid or a mixture of solid lipids, whereby the active molecules are incorporated between fatty acid chains when the lipid matrix was cooled down. SLNs have been known to have diverse advantages such as controlled drug release, drug targeting, increased drug stability, improved oral bioavailability, incorporation of lipophilic as well as hydrophilic drugs, no toxicity, avoidance of organic solvents and no problems with respect to large-scale production and sterilization [ 5 , 6 , 7 , 8 , 9 ]. SLNs can be prepared by various preparation methods such as high-pressure homogenization, high shear homogenization, ultrasound, solvent emulsification/evaporation, microemulsion technique, solvent emulsification/diffusion, double emulsion technique, membrane contactor technique and the supercritical fluid technique [ 10 ].…”

Evaluation of β-Sitosterol Loaded PLGA and PEG-PLA Nanoparticles for Effective Treatment of Breast Cancer: Preparation, Physicochemical Characterization, and Antitumor Activity

“…Particle stability was assessed by measuring size and PDI over a period of 30 days. On the other hand, stability of the nanoparticles in a biologically relevant milieu was determined by taking fixed volumes (1 mL) of the nanoparticle suspension, centrifuging at 16,900× g to pellet the nanoparticles, and re-suspending the pellet in phosphate-buffered saline (PBS, 0.01 M, pH 7.4) and PBS/FBS (50:50 v / v ) and incubated at 37 °C for 24 h in a shaker [34,35]. At predetermined time intervals, the samples were centrifuged, and pellets were washed two times with ultrapure water.…”

β‐Cyclodextrin‐Based Nanocomposite Derivatives: State of the Art in Synthesis, Characterization and Application in Molecular Recognition

“…Zeta potential and nanoparticulate configuration stability are influenced by the type and the existence of polymers, lipids, and alkyl chain characteristics of β-CD molecules. [147] In a recent study by Chen et al reports a kind of supramolecular assemblies constructed from two water-soluble and biocompatible saccharides, sulfonato-β-CD and CS shows adequate stability at temperature 10 to 70 °C. [66] β-CD has been utilized as capping agent and stabilizing agents for synthesis of copper NPs where it protects against oxidation of NPs and improves antibacterial activity.…”

“…[146] Theranostics is extensively explored in the diagnosis and treatment cancer due to unique rewards offered by stimuli-responsive nanosoldiers in malignant pathology, allowing the nanocarrier to respond specifically to the pathological 'triggers' such as pH, enzyme, redox microenvironment, temperature and small molecules for smart delivery of molecules to tumour. [147]…”

“…In formulating NPs, the stability, including its drug carrier, is a matter of concern. Zeta potential and nanoparticulate configuration stability are influenced by the type and the existence of polymers, lipids, and alkyl chain characteristics of β‐CD molecules [147] . In a recent study by Chen et al reports a kind of supramolecular assemblies constructed from two water‐soluble and biocompatible saccharides, sulfonato‐β‐CD and CS shows adequate stability at temperature 10 to 70 °C.…”